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1,10-phenanthroline
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0.24 mM, complete inhibition of plasmid nicking activity
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
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inactivation
8-hydroxychinoline 5-sulfonate
amino group modification
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aurintricarboxylic acid
-
complete inhibition at 0.005 mM
carboxylate group modification
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at pH 4.6, 50-85% loss of initial activity towards ssDNA, RNA and 3'-AMP after modification of carboxylate groups with 5-15 mM 1-ethyl-3-(dimethylaminopropyl)-carbodiimide; at pH 7.8, 55-95% loss of initial activity towards ssDNA, RNA and 3'-AMP after modification of carboxylate groups with 2-15 mM Woodward's reagent K
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citraconylation
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reversible block of amino groups, lysine residues, by citraconic anhydride
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citrate
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at 0.2 M 50% of initial activity with ssDNA
Co2+
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almost complete inhibition of 3'-AMP hydrolysis by 1 mM CoCl2
CsCl
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optimal activity 0.5-1.8 M, 27% of maximal activity at 7 M
diethyldicarbonate
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inactivation
EGTA
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1 mM, slight inhibition
glutathione
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with 4 mM of the reduced form 50% of activity with denaturated DNA
MgCl2
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50 mM, 25% inhibition
Mn2+
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6.5% of maximal activity with 0.1 mM denaturated DNA, 8.4% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
N-bromosuccinimide
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DNA and RNA protects from inactivation
N-ethylmaleimide
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with 5 mM 67% reduction of hydrolysis of polydeoxythymidylic acid
potassium citrate
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complete inhibition at 10 mM
reductive methylation
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modification of lysine residues into N,N'-dimethyl lysine
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Urea
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40% of maximal activity with single-stranded DNA with 6.6 M
additional information
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urea does not influence the enzyme at any concentrations
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2-mercaptoethanol
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with 1 mM stabilization of activity at pH 3.5 and 4.0
2-mercaptoethanol
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with 4 mM 50% of activity with denaturated DNA
5'-ATP
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50% inhibition by 0.5 mM
5'-dAMP
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50% inhibition by 0.085 mM dAMP
5'-dATP
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50% inhibition by 0.001 mM dATP
8-hydroxychinoline 5-sulfonate
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10% of maximal activity with 0.01 mM, no activity with 0.1 mM
8-hydroxychinoline 5-sulfonate
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8-hydroxychinoline 5-sulfonate
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with 1 mM 50% of maximal activity on denaturated DNA, 70% with native DNA
8-hydroxychinoline 5-sulfonate
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8% of maximal activity with 0.01 mM, no activity with 0.1 mM
8-hydroxychinoline 5-sulfonate
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50% of activity towards ssDNA with 0.005 mM
8-hydroxychinoline 5-sulfonate
-
30% of maximal activity with 0.01 mM
amino group modification
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at pH 8.0, 65-70% loss of initial activity towards ssDNA, RNA and 3'-AMP after modification of amino groups, lysine residues, with 0.5 mM 2,4,6-trinitrobenzenesulfonic acid
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amino group modification
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reaction of lysine residues with phthalaldehyde, loss of 80% of initial activity with 0.1 mM
-
Ca2+
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10 mM, complete inhibition of junction cleavage
Ca2+
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35.1% of maximal activity with 0.1 mM denaturated DNA, 74.7% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
Ca2+
-
30% of activity towards denaturated ssDNA with 1.2 mM
Ca2+
-
about 40% inhibition of hydrolysis of RNA
Ca2+
-
1 mM, slight inhibition
Ca2+
-
about 10% of activity towards ssDNA at 1 and 10 mM
Cu2+
-
46.0% of maximal activity with 0.1 mM denaturated DNA, 56.0% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Cu2+
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1 mM, slight inhibition
diphosphate
-
-
EDTA
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69% of maximum activity at 20 mM
EDTA
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complete inactiviation at 1 mM, 70% of original activity are restored by addition of 1 mM Zn2+
EDTA
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55-100% loss of initial activity with 1-10 mM
EDTA
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complete inactiviation at 1 mM, 70% of original activity are restored by addition of 1 mM Zn2+
EDTA
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almost no activity with 2 mM
EDTA
-
95% inhibition at 0.1 mM, reversed by equivalent amounts of Co2+
EDTA
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95% inhibition at 0.1 mM, reversed by equivalent amounts of Co2+; complete inactiviation at 1 mM, 70% of original activity are restored by addition of 1 mM Zn2+
EDTA
-
almost complete inactivation by 0.01 mM
EDTA
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1 mM, slight inhibition
EDTA
-
complete inactivation by incubation with 20 mM, pH 5.0
EDTA
-
reversible by Zn2+
EDTA
-
almost complete inhibition of 3'-AMP hydrolysis with 1 mM
EDTA
-
15% of maximal activity with 0.01 mM
EDTA
-
50% of activity towards ssDNA with 0.0003 mM
EDTA
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no activity after dialysis with 1 mM, no restoration by addition of Zn2+, Co2+, Mg2+, Mn2+, Ca2+ or cysteine
EDTA
-
complete inactiviation at 1 mM, 70% of original activity are restored by addition of 1 mM Zn2+; no activity after dialysis with 1 mM, no restoration by addition of Zn2+, Co2+, Mg2+, Mn2+, Ca2+ or cysteine
EDTA
-
almost complete inactivation by 0.01 mM
F-
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complete inhibition by 10 mM
F-
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18% of maximal activity with 10 mM
F-
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at 0.2 M 30% of initial activity with ssDNA
F-
-
5% of maximal activity with 10 mM
Fe2+
-
-
Fe2+
-
47.7% of maximal activity with 0.1 mM denaturated DNA, almost no activity with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Fe2+
-
about 40% inhibition of hydrolysis of RNA
Hg2+
-
-
Hg2+
-
31.5% of maximal activity with 0.1 mM denaturated DNA, 48.2% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
Hg2+
-
complete inactivation by 5 mM HgCl2
Hg2+
-
complete inhibition of 3'-AMP hydrolysis by 1 mM HgCl2
Ionic strength
-
-
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Ionic strength
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dependence of tRNA hydrolysis on salt concentration
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Ionic strength
-
inhibition of activity at ionic strength above 0.3 M
-
Ionic strength
-
about 40% of activity towards ssDNA at 0.2 M NaCl and KCl
-
Ionic strength
-
optimal 0.025-0.05 M
-
KCl
-
-
KCl
-
50% of activity with 0.019 and 0.025 mM
Mg2+
-
-
Mg2+
-
concentrations higher 2 mM
Mg2+
-
about 40% inhibition of hydrolysis of RNA with 10 mM Mg2+
Mg2+
-
1 mM, slight inhibition
Mg2+
-
reduction of activity towards ssDNA at 10 mM
NaCl
-
inhibition at concentrations higher than 200 mM
NaCl
-
optimal activity at 100 mM, 55% of maximal rate at 400 mM
NaCl
-
maximal activity at 0-100 mM, at 1 mM 71% of maximal activity
NaCl
-
inhibition of polydeoxythymidylic acid at 10-50 mM, not reversible by addition of Mg2+
NaCl
-
100 mM, 20% inhibition
NaCl
-
complete inhibition with 50 mM
NaCl
-
inhibition by low concentrations, 50% of activity with 19 and 0.025 mM
NaCl
-
inhibition at concentrations higher than 100 mM
NaCl
-
dependence of cleavage of superhelical, nicked-circular and linear DNA molecules from the NaCl concentration
NaCl
-
maximal activity 0-2 M, 40% of maximal activity at 4,4 M
NaCl
-
strong inhibition above 100 mM
phosphate
-
50% inhibition by 2 mM sodium phosphate
phosphate
-
45% of maximal activity with 20 mM potassium phosphate, 26% with 30 mM
phosphate
-
80% inhibition by 33 mM potassium phosphate, pH 7.5
phosphate
-
competitive inhibition
sodium bisphosphate
-
50% inhibition by 0.02 mM sodium bisphosphate
sodium bisphosphate
-
at 0.2 M 10% of initial activity with ssDNA
sodium dodecylsulfate
-
above 0.04% decrease of reaction rate. 60% of activity at 0.12%
sodium dodecylsulfate
-
a third of maximal activity at 0.1%, but slight activation below 0.01%
sodium dodecylsulfate
-
up to 0.6% no effect on DNA hydrolysis
sodium dodecylsulfate
-
-
sodium dodecylsulfate
-
complete inhibition with 0.2%
sodium dodecylsulfate
-
complete inactivation with 0.01% at pH 5.0
Zn2+
-
-
Zn2+
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33.7% of maximal activity with 0.1 mM denaturated DNA, 65.2% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Zn2+
-
54% of initial activity towards ssDNA with 0.1 mM Zn2+
Zn2+
-
54% of initial activity towards ssDNA with 0.1 mM Zn2+; almost complete inactivation at 1.0 mM
Zn2+
-
about 15% of activity towards ssDNA at 1 and 10 mM