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3.1.30.1: Aspergillus nuclease S1

This is an abbreviated version!
For detailed information about Aspergillus nuclease S1, go to the full flat file.

Word Map on EC 3.1.30.1

Reaction

endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products =

Synonyms

Alteromonas BAL 31 nuclease, Aspergillus oryzae S1 nuclease, Bacillus subtilis deoxyribonuclease, Bh1, CEL I, Deoxyribonuclease P1, deoxyribonuclease S1, DNase, EC 3.1.4.21, ENDO3, endonuclease 3, Endonuclease P1, Endonuclease S1, endonuclease S1 (Aspergillus), KB endonuclease, M1 nuclease, mung bean endonuclease, mung bean nuclease, mung bean nuclease I, NcNase, Neurospora crassa endonuclease, Neurospora crassa nuclease, Neurospora crassa single-strand specific endonuclease, nuclease I, nuclease S1, P1 nuclease, P4, Phage T4 endonuclease IV, Physarum polycephalum nuclease, Pisum sativum endonuclease, plant S1-like nuclease, resolvase T7, Rsn, rye germ nuclease, S1, S1 nuclease, S1-like nuclease, S1-nuclease, S1-type nuclease, SC nuclease, Schizosaccharomyces pombe endonuclease, single strand preferring nuclease, single-strand endodeoxyribonuclease, single-strand-preferring nuclease (SSP nuclease), single-strand-specific DNase, single-strand-specific endodeoxyribonuclease, single-strand-specific nuclease, single-strand-specific S1 endonuclease, single-stranded DNA specific endonuclease, single-stranded-nucleate endonuclease, SSPN, T7 endo I, T7 endonuclease, T7 endonuclease I, Ustilago maydis nuclease, wheat seedling nuclease

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.30 Endoribonucleases that are active with either ribo- or deoxyribonucleic acids and produce 5'-phosphomonoesters
                3.1.30.1 Aspergillus nuclease S1

Inhibitors

Inhibitors on EC 3.1.30.1 - Aspergillus nuclease S1

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
0.24 mM, complete inhibition of plasmid nicking activity
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
inactivation
2-mercaptoethanol
5'-ATP
5'-dAMP
5'-dATP
8-hydroxychinoline 5-sulfonate
amino group modification
-
aurintricarboxylic acid
-
complete inhibition at 0.005 mM
beta-mercaptoethanol
-
-
carboxylate group modification
-
at pH 4.6, 50-85% loss of initial activity towards ssDNA, RNA and 3'-AMP after modification of carboxylate groups with 5-15 mM 1-ethyl-3-(dimethylaminopropyl)-carbodiimide; at pH 7.8, 55-95% loss of initial activity towards ssDNA, RNA and 3'-AMP after modification of carboxylate groups with 2-15 mM Woodward's reagent K
-
citraconylation
-
reversible block of amino groups, lysine residues, by citraconic anhydride
-
citrate
-
at 0.2 M 50% of initial activity with ssDNA
Co2+
-
almost complete inhibition of 3'-AMP hydrolysis by 1 mM CoCl2
CsCl
-
optimal activity 0.5-1.8 M, 27% of maximal activity at 7 M
diethyldicarbonate
-
inactivation
diphosphate
dithiothreitol
-
-
EGTA
-
1 mM, slight inhibition
glutathione
-
with 4 mM of the reduced form 50% of activity with denaturated DNA
iodoacetamide
-
-
Ionic strength
-
MgCl2
-
50 mM, 25% inhibition
Mn2+
-
6.5% of maximal activity with 0.1 mM denaturated DNA, 8.4% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
N-bromosuccinimide
-
DNA and RNA protects from inactivation
N-ethylmaleimide
-
with 5 mM 67% reduction of hydrolysis of polydeoxythymidylic acid
phosphate
Polyvinyl sulfate
-
-
potassium citrate
-
complete inhibition at 10 mM
reductive methylation
-
modification of lysine residues into N,N'-dimethyl lysine
-
sodium bisphosphate
sodium dodecylsulfate
Urea
-
40% of maximal activity with single-stranded DNA with 6.6 M
additional information
-
urea does not influence the enzyme at any concentrations
-