3.1.26.13: retroviral ribonuclease H

This is an abbreviated version!
For detailed information about retroviral ribonuclease H, go to the full flat file.

Reaction

Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus. =

Synonyms

HIV reverse transcriptase ribonuclease H, HIV reverse transcriptase-associated RNase H, HIV RNase H, HIV RT RNaseH, HIV RT-associated RNase H, HIV-1 reverse transcriptase, HIV-1 reverse transcriptase ribonuclease H, HIV-1 reverse transcriptase RNase H, HIV-1 RH, HIV-1 ribonuclease H, HIV-1 ribonuclease H domain, HIV-1 RNase H, HIV-1 RT-associated RNase H, human immunodeficiency virus reverse transcriptase, human immunodeficiency virus reverse transcriptase-associated ribonuclease H, human immunodeficiency virus reverse transcriptase-associated RNase H, human immunodeficiency virus RT-associated RNase H, M-MuLV RT RNase H, reverse transcriptase, reverse transcriptase-associated ribonuclease H, reverse transcriptase-associated RNase H, reverse-transcriptase-associated ribonuclease H, ribonuclease H, ribonuclease H domain in HIV-1 reverse transcriptase, RNase H, RNase H activity of HIV-1 reverse transcriptase, RNase H domain of HIV-1 reverse transcriptase, RNase H of HIV-1 subtype C, RNaseH, RNH, RNHHIV, RT RNase H, RT-RNase H

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.26 Endoribonucleases producing 5'-phosphomonoesters

                3.1.26.13 retroviral ribonuclease H

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3	Activating Compound
80	AA Sequence
11	Application
1	CAS Registry Number
21	Cloned(Commentary)
14	Crystallization (Commentary)
56	General Information
517	IC50 Value
556	Inhibitors
7	kcat/KM [mM/s]
3	Ki Value [mM]
20	KM Value [mM]
48	Metals/Ions
4	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
136	Organism
388	PDB ID
22	pH Optimum
2	Posttranslational Modification
291	Protein Variants
14	Purification (Commentary)
2	Reaction
120	Reference
1	Renatured (Commentary)
3	Source Tissue
3	Specific Activity [micromol/min/mg]
85	Substrates and Products (Substrate)
11	Subunits
154	Synonyms
20	Temperature Optimum [°C]
18	Temperature Stability [°C]
13	Turnover Number [1/s]

Purification

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Purification on EC 3.1.26.13 - retroviral ribonuclease H

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

a HIV-1 RNase H specific inhibitor from the hydroxyimide class, that contains a primary amine with a long nonfunctional linker, is conjugated to NHS-activated HiTrap HP resins for use in affinity chromatographic HIV reverse transcriptase purifcation. Purification of untagged HIV-1 RT using an avidin affinity column in presence of Mg2+, method evaluations, overview

Human immunodeficiency virus 1

-

716827

HiTrap column chromatography and Superdex 200 gel filtration

Human immunodeficiency virus 1

-

730486

Ni-NTA agarose column chromatography

Human immunodeficiency virus 1

-

730494

Ni-NTA column chromatography and heparin column chromatography

Human immunodeficiency virus 1

P03366

730522

recombinant enzyme mutant C280S

Human immunodeficiency virus 1

-

750467

recombinant His-tagged enzyme by nickel affinity chromatography, the His6 tag is removed by proteolysis with thrombin, followed by gel filtration

Human immunodeficiency virus 1

P0C6F2

716064

recombinant His-tagged enzyme from Escherichia coli strain M15 by nickel and heparin affinity chromatography

Human immunodeficiency virus type 1 group M subtype B

P03366

750331

recombinant His-tagged p66 or p51 HIV-1 RT DNA fragment from Escherichia coli strain BL21(DE3)pLys by nickel affinity chromatography, expression of a heterodimer of wild-type or F160S and C280S mutant p66 and p51 using plasmid vector RT69A in Escherichia coli strain Rosetta

Human immunodeficiency virus 1

-

714475

recombinant His6-tagged p66/p51 HIV-1 reverse transcriptase 52A variant C280S by nickel affinity chromatography, the tag is cleaved by HRV14 3C protease

Human immunodeficiency virus 1

-

715864

recombinant mutant constructs by gel filtration

Human immunodeficiency virus 1

P04585

751727

recombinant N-terminally His-tagged p66 and p51 subunit mutants C282S from Escherichia coli strain BL21 Rosetta by nickel affinity chromatography, dialysis, heparin affinity chromatography, and gel filtration

Human immunodeficiency virus 1

-

751738

recombinant N-terminally His6-SUMO-fused wild-type and mutant RNase H RNH domains (residues 427-560) from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography and gel filtration, the N-terminal His6-SUMO fusion is removed by digestion with histidine tagged ubiquitin-like-protein specific protease (ULP1)

Human immunodeficiency virus 1

P03366

752137

recombinant wild-type enzyme and chimeric mutants from Escherichia coli strain MIC2067 (DE3) by dialysis of cell-free enzyme extract, anion and cation exchange chromatography, dialysis, and gel filtration

Human immunodeficiency virus 1

-

751537

soluble recombinant RNase H, in an N-terminally His-tagged construct, p51-G-TCR construct, designed to encode the p51 subunit joined by a linker to the thumb (T), connection (C), and RNase H (R) domains of p66, from Escherichia coli strain MIC2067(DE3) by nickel affinity chromatography

Human immunodeficiency virus 1

-

713774