3.1.26.10: ribonuclease IX

This is an abbreviated version!
For detailed information about ribonuclease IX, go to the full flat file.

Reaction

endonucleolytic cleavage of poly(U) or poly(C) to fragments terminated by 3'-hydroxy and 5'-phosphate groups =

Synonyms

EC-RNase, pancreatic-type ribonuclease, poly(U)- and poly(C)-specific endoribonuclease, RNase 1

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.26 Endoribonucleases producing 5'-phosphomonoesters

                3.1.26.10 ribonuclease IX

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Results	in table
1	CAS Registry Number
1	General Stability
6	Inhibitors
1	Localization
7	Metals/Ions
5	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
5	Organism
2	pH Optimum
1	pH Range
1	pI Value
5	Purification (Commentary)
1	Reaction
1	Reaction Type
5	Reference
11	Source Tissue
4	Specific Activity [micromol/min/mg]
1	Storage Stability
16	Substrates and Products (Substrate)
3	Subunits
4	Synonyms
1	Temperature Range [°C]
1	Temperature Stability [°C]

Substrates Products

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Substrates Products on EC 3.1.26.10 - ribonuclease IX

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

mRNA + H2O

?

4 entries

poly(C) x (dG)12-18 polynucleotide template primer + H2O

?

2 entries

RNA + H2O

?

Ceratitis capitata

-

RNA isolated from six-day-old larvae or rat liver

134482

-

-

?

rRNA + H2O

?

Saccharomyces cerevisiae

-

26S, 18S and 5S rRNA, processing of rRNA, naked rRNA molecules are sensitive to the purified enzyme, enzyme has no activity to the ribosome integrated rRNA

646296

-

-

?

synthetic homopolynucleotide + H2O

?

4 entries

additional information

?

-

3 entries

mRNA + H2O

?

Ceratitis capitata

-

control of protein synthesis in eucaryotes

134482

-

-

?

mRNA + H2O

?

Ceratitis capitata

-

translational control

646294

-

-

?

mRNA + H2O

?

Saccharomyces cerevisiae

-

-

646296

-

-

?

mRNA + H2O

?

Saccharomyces cerevisiae

-

mRNA obtained from in vitro transcription

646296

-

-

?

poly(C) x (dG)12-18 polynucleotide template primer + H2O

?

Ceratitis capitata

-

due to the presence of the poly(C) tail

646294

-

-

?

poly(C) x (dG)12-18 polynucleotide template primer + H2O

?

Saccharomyces cerevisiae

-

-

646296

-

-

?

synthetic homopolynucleotide + H2O

?

Ceratitis capitata

-

-

646295

-

-

?

synthetic homopolynucleotide + H2O

?

Ceratitis capitata

-

acts on poly(U) and poly(C) with a higher affinity for the latter

134482, 646294

-

-

?

synthetic homopolynucleotide + H2O

?

Homo sapiens

-

acts on poly(U) and poly(C) with a higher affinity for the latter

646297

-

-

?

synthetic homopolynucleotide + H2O

?

Saccharomyces cerevisiae

-

acts on poly(U) and poly(C) with a higher affinity for the latter

646296

-

-

?

additional information

?

-

Ceratitis capitata

-

specifically degrades poly(U) and poly(C), fails to degrade other single-stranded homopolyribonucleotides

134482

-

-

?

additional information

?

-

Homo sapiens

-

endowed with non-digestive functions and involved in vascular homeostasis

646297

-

-

?

additional information

?

-

Saccharomyces cerevisiae

-

no catalytic activity observed with tRNA and double stranded substrates, little or no reaction with poly(A), poly(I), poly(G), poly(dG)xpoly(dG-dC), and poly(dG-dC)xpoly(dG-dC)

646296

-

-

?