3.1.22.5: deoxyribonuclease X

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For detailed information about deoxyribonuclease X, go to the full flat file.

Reaction

endonucleolytic cleavage of supercoiled plasma DNA to linear DNA duplexes =

Synonyms

Escherichia coli endodeoxyribonuclease, Escherichia coli endodeoxyribonuclease X

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.22 Endodeoxyribonucleases producing 3'-phosphomonoesters

                3.1.22.5 deoxyribonuclease X

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Results	in table
1	CAS Registry Number
8	Inhibitors
3	Metals/Ions
1	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
177	Organism
1	pH Optimum
1	Purification (Commentary)
1	Reaction
1	Reaction Type
2	Reference
8	Substrates and Products (Substrate)
2	Subunits
2	Synonyms

Metals Ions

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Metals Ions on EC 3.1.22.5 - deoxyribonuclease X

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Ca2+

Escherichia coli

-

absolute requirement for a divalent cation, optimum concentration: 1.5 mM, inhibition at higher concentrations

646292

Mg2+

Escherichia coli

-

absolute requirement for a divalent cation, optimum concentration: 5 mM, inhibition at higher concentrations

646292

additional information

Escherichia coli

-

very little activity with Mn2+ or Zn2+

646292