3.1.21.9: T4 deoxyribonuclease IV

This is an abbreviated version!
For detailed information about T4 deoxyribonuclease IV, go to the full flat file.

Reaction

endonucleolytic cleavage of the 5' phosphodiester bond of deoxycytidine in single-stranded DNA =

Synonyms

bacteriophage-T4-induced endonuclease IV, denB, EndoIV, endonuclease IV, T4 endonuclease IV

ECTree

3 Hydrolases

3.1 Acting on ester bonds

3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters

3.1.21.9 T4 deoxyribonuclease IV

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Results	in table
1	Activating Compound
6	AA Sequence
1	Cloned(Commentary)
2	General Information
7	Inhibitors
3	kcat/KM [mM/s]
22	KM Value [mM]
7	Metals/Ions
3	Natural Substrates/ Products (Substrates)
8	Organism
4	pH Optimum
2	pH Range
3	Purification (Commentary)
1	Reaction
8	Reference
1	Specific Activity [micromol/min/mg]
32	Substrates and Products (Substrate)
6	Synonyms
1	Temperature Optimum [°C]
3	Turnover Number [1/s]

Reference

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Reference on EC 3.1.21.9 - T4 deoxyribonuclease IV

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134240

Sadowski, P.D.; Hurwitz, J.

Enzymatic breakage of deoxyribonucleic acid. II. Purification and properties of endonuclease IV from T4 phage-infected Escherichia coli

J. Biol. Chem.

244

6192-6198

1969

Tequatrovirus T4

4900512

682168

Hirano, N.; Ohshima, H.; Takahashi, H.

Biochemical analysis of the substrate specificity and sequence preference of endonuclease IV from bacteriophage T4, a dC-specific endonuclease implicated in restriction of dC-substituted T4 DNA synthesis

Nucleic Acids Res.

4743-4751

2006

Tequatrovirus T4

16971463

682182

Ohshima, H.; Hirano, N.; Takahashi, H.

A hexanucleotide sequence (dC1-dC6 tract) restricts the dC-specific cleavage of single-stranded DNA by endonuclease IV of bacteriophage T4

Nucleic Acids Res.

6681-6689

2007

Tequatrovirus T4

17940096

727415

Bernardi, A.; Maat, J.; De Waard, A.; Bernardi, G.

Preparation and specificity of endonuclease IV induced by bacteriophage T4

Eur. J. Biochem.

175-179

1976

Tequatrovirus T4

782881

727492

Ling, V.

Partial digestion of 32P-fd DNA with T4 endonuclease IV

FEBS Lett.

50-54

1971

Tequatrovirus T4

11946172

727596

Carlson, K.; Overvatn, A.

Bacteriophage T4 endonucleases II and IV, oppositely affected by dCMP hydroxymethylase activity, have different roles in the degradation and in the RNA polymerase-dependent replication of T4 cytosine-containing DNA

Genetics

114

669-685

1986

Tequatrovirus T4

2431959

727716

Saporito, S.M.; Gedenk, M.; Cunningham, R.P.

Role of exonuclease III and endonuclease IV in repair of pyrimidine dimers initiated by bacteriophage T4 pyrimidine dimer-DNA glycosylase

J. Bacteriol.

171

2542-2546

1989

Tequatrovirus T4

2468648

727799

Sadowski, P.; Bakyta, I.

T4 endonuclease IV. Improved purification procedure and resolution from T4 endonuclease 3

J. Biol. Chem.

247

405-412

1972

Tequatrovirus T4

4550601