3.1.21.9: T4 deoxyribonuclease IV

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For detailed information about T4 deoxyribonuclease IV, go to the full flat file.

Reaction

endonucleolytic cleavage of the 5' phosphodiester bond of deoxycytidine in single-stranded DNA =

Synonyms

bacteriophage-T4-induced endonuclease IV, denB, EndoIV, endonuclease IV, T4 endonuclease IV

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters

                3.1.21.9 T4 deoxyribonuclease IV

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Results	in table
1	Activating Compound
6	AA Sequence
1	Cloned(Commentary)
2	General Information
7	Inhibitors
3	kcat/KM [mM/s]
22	KM Value [mM]
7	Metals/Ions
3	Natural Substrates/ Products (Substrates)
8	Organism
4	pH Optimum
2	pH Range
3	Purification (Commentary)
1	Reaction
8	Reference
1	Specific Activity [micromol/min/mg]
32	Substrates and Products (Substrate)
6	Synonyms
1	Temperature Optimum [°C]
3	Turnover Number [1/s]

Inhibitors

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Inhibitors on EC 3.1.21.9 - T4 deoxyribonuclease IV

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

(NH4)2SO4

Tequatrovirus T4

-

0.05 M, 70% inhibition

134240

K3PO4

Tequatrovirus T4

-

0.05 M, 80% inhibition

134240

KCl

2 entries

NaCl

2 entries

NH4Cl

Tequatrovirus T4

-

0.05 M, 47% inhibition

134240

KCl

Tequatrovirus T4

-

0.05 M, 80% inhibition

134240

KCl

Tequatrovirus T4

-

50 mM, 20% residual activity

682168

NaCl

Tequatrovirus T4

-

0.05 M, 85% inhibition

134240

NaCl

Tequatrovirus T4

-

50 mM, 15% residual activity

682168