3.1.21.6: CC-preferring endodeoxyribonuclease
This is an abbreviated version!
For detailed information about CC-preferring endodeoxyribonuclease, go to the full flat file.
Word Map on EC 3.1.21.6
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3.1.21.6
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bacteriophage
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nick
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apurinic
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exonuclease
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endonucleases
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phosphodiester
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depurinated
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endonucleolytic
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cytosine-containing
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incise
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superhelical
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coliphage
- 3.1.21.6
- bacteriophage
- nick
-
apurinic
-
exonuclease
- endonucleases
-
phosphodiester
-
depurinated
-
endonucleolytic
-
cytosine-containing
-
incise
-
superhelical
-
coliphage
Reaction
Endonucleolytic cleavage to give 5'-phosphooligonucleotide end-products, with a preference for cleavage within the sequence CC =
Synonyms
Deoxyriboendonuclease, DNA-adenine-transferase, EC 3.1.4.30, endodeoxyribonuclease, endonuclease II, endonuclease NX, endonuclease T, Escherichia coli endonuclease II, Nuclease, endodeoxyribo-, R1Bm EN, R1Bm endonuclease, Redoxyendonuclease, Streptomyces glaucescens exocytoplasmic dodeoxyribonuclease, VirD1/D2 endonuclease
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Substrates Products
Substrates Products on EC 3.1.21.6 - CC-preferring endodeoxyribonuclease
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REACTION DIAGRAM
28S rDNA + H2O
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R1Bm EN cleaves both strands of the 28S rDNA sequence at the target site, 5'-A(G/C)(A/T)!(A/G)T-3' is the consensus sequence, thus cleavage site
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Lambda DNA
Double-stranded fragments between 7 and 24 bases with 5' and/or 3'-protruding single-stranded tails
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pBR322 DNA + H2O
DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends, respectively
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preferably binds to the dinucleotide 5'-CC-3' and nicks DNA between both cytosines
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double-stranded DNA
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Virus NYs-1
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the enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-CIIIC-3' sequences but not 5'-IIICC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5-CC-3' sequences on opposite strands are close enough for the two strands to separate. When the 5'-CC-3' sequence on opposite strands are further apart only a portion of the strands separate
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catalyzes the endonucleolytic cleavage of both the double- as well as the single-strand DNA non-specifically
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additional information
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Virus NYs-1
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the enzyme does not cleave ssDNA and definitely does not cleave ssRNA or dsRNA
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