3.1.21.5: type III site-specific deoxyribonuclease
This is an abbreviated version!
For detailed information about type III site-specific deoxyribonuclease, go to the full flat file.
Word Map on EC 3.1.21.5
Reaction
endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates =
Synonyms
ATP-dependent type III restriction endonuclease, BceSI, BsaHI, cj0031, EC 3.1.23, EC 3.1.24, EcoP1, EcoP15, EcoP15I, EcoPI, HinFIII, LlaFI, MmyCI, More, NgoAXP, PhaBI, PspGI, PstII, R.EcoP15I, R.MmyCI, REase, restriction endonuclease PstII, restriction-modification system, StyLTI, type III DNA restriction/modification enzyme, type III R-M enzyme, type III R/M enzyme, type III RE, type III restriction endonuclease, type III restriction enzyme, type III restriction-modification enzyme, type III restriction-modification system, type III RM system, type III testriction-modification enzyme, type III-like restriction endonuclease
ECTree
3.1.21.5 type III site-specific deoxyribonucleaseAdvanced search results
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Engineering on EC 3.1.21.5 - type III site-specific deoxyribonuclease
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
G448S
-
mutant of Mod subunit, not able to bind S-adenosyl-L-methionine, no DNA cleavage
D354A
mutant shows reduced activity compared to the wild type enzyme
E350A
mutant shows reduced activity compared to the wild type enzyme
F353A
mutant shows reduced activity compared to the wild type enzyme
P349A
mutant shows reduced activity compared to the wild type enzyme
Q344A
mutant shows activity similar to wild type enzyme
R352A
mutant shows activity similar to the wild type enzyme
S348A
mutant shows activity similar to the wild type enzyme
additional information
additional information
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mutant c2-134, unable to cleave DNA, mutant c2-440, poor ability to cleave DNA
additional information
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study on the predicted linker region between the two domains of the restriction subunit by insertional mutagenesis. Introduction of up to 18 amino acids in the N- and C-terminal region of the linker. The region tolerated the introduced genetic alterations without loss of catalytic function or changes in cleavage position
additional information
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analysis of several strains for phase variable methyltransferase activity, mod, and restriction endonuclease activity. Of 41 strains in the survey that contain a phase variable mod gene, seven have an obvious mutation in the restriction domain and appear to be dedicated phasevarions. The remaining 15 strains that do not contain a phase variable mod gene are likely to be dedicated, functional, type III restriction-modification systems
