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3.1.21.5: type III site-specific deoxyribonuclease

This is an abbreviated version!
For detailed information about type III site-specific deoxyribonuclease, go to the full flat file.

Word Map on EC 3.1.21.5

Reaction

endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates =

Synonyms

ATP-dependent type III restriction endonuclease, BceSI, BsaHI, cj0031, EC 3.1.23, EC 3.1.24, EcoP1, EcoP15, EcoP15I, EcoPI, HinFIII, LlaFI, MmyCI, More, NgoAXP, PhaBI, PspGI, PstII, R.EcoP15I, R.MmyCI, REase, restriction endonuclease PstII, restriction-modification system, StyLTI, type III DNA restriction/modification enzyme, type III R-M enzyme, type III R/M enzyme, type III RE, type III restriction endonuclease, type III restriction enzyme, type III restriction-modification enzyme, type III restriction-modification system, type III RM system, type III testriction-modification enzyme, type III-like restriction endonuclease

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters
                3.1.21.5 type III site-specific deoxyribonuclease

Cloned

Cloned on EC 3.1.21.5 - type III site-specific deoxyribonuclease

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into pQE-16 plasmid vector that provides the enzyme with a C-terminal 6His-tag
-
expressed in Escherichia coli strain ER2566
expressed in Escherichia coli strain ER2744
-
expression in Escherichia coli
expression in Escherichia coli BL21(DE3) cells