3.1.21.4: type II site-specific deoxyribonuclease
This is an abbreviated version!
For detailed information about type II site-specific deoxyribonuclease, go to the full flat file.
Word Map on EC 3.1.21.4
-
3.1.21.4
-
endonucleases
-
phage
-
lambda
-
agarose
-
strand
-
rrna
-
bacteriophage
-
pcr-rflp
-
viruses
-
serotype
-
pulsed-field
-
single-stranded
-
spacers
-
cosmid
-
amplicons
-
adenovirus
-
outbreak
-
simplex
-
herpes
-
mtdnas
-
cytosine
-
duplex
-
serological
-
pcr-based
-
nested
-
epstein-barr
-
cpg
-
caucasian
-
methylase
-
reaction-restriction
-
herpesvirus
-
virion
-
satellite
-
tetracycline
-
nosocomial
-
32p-labeled
-
proviral
-
beta-globin
-
simian
-
ethidium
-
globin
-
ampicillin
-
discriminatory
-
dna-dna
-
unmethylated
-
supercoiled
-
serogroups
-
biotype
-
subfragment
-
analysis
-
biotechnology
-
beta-thalassemia
-
molecular biology
- 3.1.21.4
- endonucleases
- phage
- lambda
- agarose
- strand
- rrna
- bacteriophage
-
pcr-rflp
- viruses
-
serotype
-
pulsed-field
-
single-stranded
-
spacers
-
cosmid
-
amplicons
- adenovirus
-
outbreak
- simplex
- herpes
- mtdnas
- cytosine
- duplex
-
serological
-
pcr-based
-
nested
-
epstein-barr
- cpg
-
caucasian
-
methylase
-
reaction-restriction
- herpesvirus
- virion
- satellite
- tetracycline
-
nosocomial
-
32p-labeled
-
proviral
- beta-globin
-
simian
- ethidium
- globin
- ampicillin
-
discriminatory
-
dna-dna
-
unmethylated
-
supercoiled
- serogroups
-
biotype
-
subfragment
- analysis
- biotechnology
- beta-thalassemia
- molecular biology
Reaction
endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates =
Synonyms
AatII, AcuI, acuIRM, AfeI, AflII, AflIII, aflIIR, AgeI, AhaIII, AlfI, AloI, AluI, AlwNI, Apa BI, Apa CI, Apa DI, ApaI, ApaLI, apaLIR, AsuI, AsuII, AvaI, AvaII, AvaIII, AvrII, BaeI, BamHI, bamHIR, BbeI, BbvCI, BbvI, BbvII, bbvIR, BcefI, BcgI, BclI, Bfi2411I, BgII, BglI, BglII, bglIIR, BinI, BmrI, BplI, BpmI, bpmIR, BpuEI, BpuIOI, BsaAI, BsaBI, BsaWI, BsaXI, Bse634I, BsePI, BseRI, BseRI endonuclease, BsgI, BsmAI, BsmBI, BsmI, BsoBI, Bsp CI, BspD6I, BspGI, BspHI, BspI, BspMI, BSPMII, BspRI, BsrI, BssSI, BstEII, BstF5I, BstII, BstXI, BstYI, Bsu2413I, BtsI, CauII, Cfr101, Cfr10I, CfrBI, CfrI, CjeI, ClaI, CspCI, CstMI, CviAI, CviJI, DdeI, DNA restriction endonuclease, DNA restriction enzyme, DpnI, DpnII, DraII, DraIII, DrdI, DrdII, DsaI, EC 3.1.23, EC 3.1.24, EciI, Eco1524I, Eco311, Eco31I, Eco47III, Eco571, Eco57I, eco57IR, EcoO109I, EcoP15I, ecoP15Ires, EcoRI, EcoRII, ecoRIR, EcoRV, EcoVIII, EgeI, EheI, Endonuclease AbrI, Endonuclease AccI, Endonuclease AgeI, Endonuclease ApaLI, Endonuclease AvaI, Endonuclease BamHI, Endonuclease BanI, Endonuclease BglI, Endonuclease BglII, Endonuclease BsoBI, Endonuclease Bsp6I, Endonuclease BstVI, Endonuclease BsuBI, Endonuclease BsuFI, Endonuclease BsuRI, Endonuclease CeqI, Endonuclease Cfr10I, Endonuclease Cfr9I, Endonuclease CfrBI, Endonuclease CviAII, Endonuclease CviJI, Endonuclease DdeI, Endonuclease DpnI, Endonuclease DpnII, Endonuclease Eco47I, Endonuclease Eco47II, Endonuclease EcoRI, Endonuclease EcoRII, Endonuclease EcoRV, Endonuclease FokI, Endonuclease HaeII, Endonuclease HaeIII, Endonuclease HgAI, Endonuclease HgiBI, Endonuclease HgiCI, Endonuclease HgiCII, Endonuclease HgiDI, Endonuclease HgiEI, Endonuclease HgiGI, Endonuclease HhaII, Endonuclease HincII, Endonuclease HindII, Endonuclease HindIII, Endonuclease HindVP, Endonuclease HinfI, Endonuclease HpaI, Endonuclease HpaII, Endonuclease HphI, Endonuclease KpnI, Endonuclease LlaDCHI, Endonuclease MamI, Endonuclease MboI, Endonuclease MboII, Endonuclease MjaI, Endonuclease MjaII, Endonuclease MjaIII, Endonuclease MjaIV, Endonuclease MjaV, Endonuclease MjaVIP, Endonuclease MspI, Endonuclease MthTI, Endonuclease MthZI, Endonuclease MunI, Endonuclease MwoI, Endonuclease NaeI, Endonuclease NgoBI, Endonuclease NgoBV, Endonuclease NgoFVII, Endonuclease NgoMIV, Endonuclease NgoPII, Endonuclease NlaIII, Endonuclease NlaIV, Endonuclease NmeDIP, Endonuclease NspV, Endonuclease PaeR7I, Endonuclease PstI, Endonuclease PvuI, Endonuclease PvuII, Endonuclease RsrI, Endonuclease SacI, Endonuclease SalI, Endonuclease Sau3AI, Endonuclease Sau96I, Endonuclease ScaI, Endonuclease ScrFI, Endonuclease SfiI, Endonuclease SinI, Endonuclease SmaI, Endonuclease SsoII, Endonuclease StsI, Endonuclease TaqI, Endonuclease TthHB8I, Endonuclease XamI, Endonuclease XcyI, endoR*Bsp, Esp3I, EspI, FauI, FinI, Fnu4HI, FnuSII, FokI, fokIR, FseI, GdiII, GGCC-specific restriction endonuclease, GsuI, HaeI, HaeII, HaeIII, HaeVI, HgaI, HgiAI, HgiCI, HgiEI, HgiEII, HgiJII, HhaI, HhaII, HincII, HindII, HindIII, hindIIIR, HinfI, HinP1I, HpaI, HpaII, HphI, Hpy188I, Hpy8I, Hpy99II, Hpy99IV, Hpy99VIIIP, HpyAXII, HpyF17I, KasI, KpnI, KpnIR, Ksp6321, LlaDI, LlaII, LmoJ2, LmoJ3, MaeI, MaeII, MaeIII, MboI, MboII, McaTI, McrI, MfeI, MjaIV, MjaV, MluI, Mly113I, MlyI, MmeI, mmeIRM, MmoSTI, MnlI, More, MseI, MspA1I, MspI, MstI, MunI, Mva1269I, Mva1269I restriction endonuclease, MvaI, MwoI, NaeI, NarI, NcoI, ncoIR, NdeI, NgoMIV, NheI, NlaIII, NlaIV, NmeDI, NmeDIP, NotI, NruI, NspBII, NspV, Nt.BspD6I, nuclease, deoxyribonucleic restriction endo-, nuclease, restriction endodeoxyribo-, PabI, PflMI, PhoI, PleI, PmaCI, PPII, PpuMI, PsasM2I, PshAI, PspGI, PstI, pstIR, PvuI, PvuII, R.AbrI, R.AccI, R.AgeI, R.ApaLI, R.AvaI, R.BamHI, R.BanI, R.BglI, R.BglII, R.BsoBI, R.Bsp6I, R.BstVI, R.BsuBI, R.BsuFI, R.BsuRI, R.CeqI, R.Cfr10I, R.Cfr9I, R.CfrBI, R.CviAII, R.CviJI, R.DdeI, R.DpnI, R.DpnII, R.Eco47I, R.Eco47II, R.EcoRI, R.EcoRII, R.EcoRV, R.FokI, R.HaeII, R.HaeIII, R.HgAI, R.HgiBI, R.HgiCI, R.HgiCII, R.HgiDI, R.HgiEI, R.HgiGI, R.HhaII, R.HincII, R.HindII, R.HindIII, R.HindVP, R.HinfI, R.HpaI, R.HpaII, R.HphI, R.KpnI, R.LlaDCHI, R.MamI, R.MboI, R.MboII, R.MjaI, R.MjaII, R.MjaIII, R.MjaIV, R.MjaV, R.MjaVIP, R.MspI, R.MthTI, R.MthZI, R.MunI, R.MwoI, R.NaeI, R.NgoBI, R.NgoBV, R.NgoFVII, R.NgoI, R.NgoMIV, R.NgoPII, R.NgoV, R.NgoVII, R.NlaIII, R.NlaIV, R.NmeDIP, R.NspV, R.PaeR7I, R.PstI, R.PvuI, R.PvuII, R.RsrI, R.SacI, R.SalI, R.Sau3AI, R.Sau96I, R.ScaI, R.ScrFI, R.SfiI, R.SinI, R.SmaI, R.SsoII, R.StsI, R.TaqI, R.TthHB8I, R.XamI, R.XcyI, REase, restriction endodeoxyribonuclease, restriction endonuclease, restriction endonuclease EcoRV, restriction endonuclease SuaI, restriction enzyme, RleAI, RsaI, SacI, SacII, SacIR, SalI, Sau3AI, sau96I, SauI, ScaI, ScrFI, SduI, SecI, SfaNI, SfeI, SfiI, SfoI, SgrAI, Sho27844P, site-specific endonuclease BME142I, site-specific endonuclease Bme216I, site-specific endonuclease BmeI, site-specific type II endonuclease, SmaI, SmuI, SnaBI, SnaI, SpeI, SphI, SpiI, ss.BspD6I, Sse9I, SspI, StuI, StyI, SuaI, SuiI, TaqI, TaqII, TfiI, Tsp451, TspEI, TstI, Tth111I, Tth111II, type II ENase, type II REase, type II restriction endonuclease, type II restriction endonuclease EcoO109I, type II restriction enonuclease, type II restriction enzyme, type II restriction-modification protein, type II restriction-modification system, type II RM system, type IIB restriction endonuclease, type IIE restriction endonuclease, type IIF restriction endonuclease, type IIP REase, type IIP restriction endonuclease, Type IIS REase, type IIS restriction endonuclease, type IIS restriction enzyme, type IIT restriction endonuclease BbvCI, typeIIS restriction endonuclease, Uba1105I, Uba1108I, UbaLAI, UpaP162, UPV_230, VspI, XbaI, XcmI, XhoI, XhoII, XmaIII, XmnI
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3.1.21.4 type II site-specific deoxyribonucleaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.1.21.4 - type II site-specific deoxyribonuclease
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
DNA containing F5dC + H2O
?
perfectly hydrolyzes the DNA containing 5-fluoro-dC
-
-
?
DNA containing F5dU + H2O
?
perfectly hydrolyzes the DNA containing 5-fluoro-dU
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with 3-base 5'-overhangs
Q5D6Y5; Q5D6Y4
the enzyme recognizes and cleaves the seven base pair sequence 5'-CCTCAGC-3', generating 3-base, 5'-overhangs
-
-
?
pBR322DNA + H2O
pBR322 DNA fragments
-
the tetranucleotide GGCC can be cleaved by SuaI either symmetrically or nonsymmetrically, thus producing termini with a single-stranded end
-
-
?
DNA + H2O
?
-
primary function is the inactivation of foreign DNA invading bacteria
-
-
?
DNA + H2O
?
-
-
the isolated C-terminal domain dimer has an interface that binds a single cognate DNA molecule whereas the N-terminal domain is a monomer that also binds a single copy of cognate DNA
-
?
DNA + H2O
?
-
sequence-specific endonucleolytic digestion of infecting DNA
-
-
?
DNA + H2O
?
the enzyme recognizes a degenerated sequence 5'-W/CCGGW-3' (W stands for A or T and / denotes the cleavage site). It belongs to a large family of restriction enzymes that contain a conserved CCGG tetranucleotide in their target sites. It requires binding of two target sites for the optimal catalytic activity
-
-
?
DNA + H2O
?
-
sequence-specific endonucleolytic digestion of infecting DNA
-
-
?
DNA + H2O
?
the enzyme is resilient to specificity changes at the first position of the recognition sequence (5'-TCCRAC-3')
-
-
?
DNA + H2O
?
-
the enzyme recognizes interrupted palindromes, i.e., 5'-CCNGG-3' sequences and cleaves DNA leaving 5-nucleotide long, single-stranded, 5'-cohesive ends
-
-
?
DNA + H2O
?
-
sequence-specific endonucleolytic digestion of infecting DNA
-
-
?
DNA + H2O
?
-
recognition site is GGCC, enzyme does not cut Sulfolobus acidocaldarius DNA, as the recognition site in this DNA contains modified nucleotides
-
-
?
DNA + H2O
?
-
enzyme cuts DNA at the recognition site GcwGC, cleavage occurs after the first guanosine base
-
-
?
DNA + H2O
?
-
enzyme cuts DNA at the recognition site GcwGC, cleavage occurs after the first guanosine base
-
-
?
DNA + H2O
?
the enzyme is specific for the pseudosymmetric DNA sequence 5'-CC/WGG-3' (where W = A/T, and / marks the cleavage position). UbaLAI requires two recognition sites for optimal activity, and, like other type IIE enzymes, uses one copy of a recognition site to stimulate cleavage of a second copy. It is proposed that during the reaction the UbaLAI N-terminal domain acts as a handle that tethers the monomeric UbaLAI C-terminal domain to the DNA, thereby helping UbaLAI-C to perform two sequential DNA nicking reactions on the second recognition site during a single DNA-binding event
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
enzyme ApaBI recognizes 35 cleavage sites on bacteriophage lamda DNA, 20 sites on adenovirus-2 DNA and 2 sites on plasmid pBR322 DNA. The recognition sequence is 5'-GCANNNNN/TGC-3'\\3'-CGT/NNNNNACG-5', enzyme ApaDI has 6 sites of cleavage on the pBR327 DNA, 7 sites on pAT153 DNA and more than 20 sites on bacteriophage lamda DNA. ApaCI cleaves linear lambda DNA at five sites, circular pBR DNA, pMRFb DNA and pHC 624 DNA at one site
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
mechanochemical model of induced-fit reactions on DNA. Strongly decreased association rate is obtained on streched DNA
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of BamHI: GGATCC. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
BspD6I cleaves both DNA strands within the recognition sequence
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
BspD6I cleaves both DNA strands within the recognition sequence
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
the enzyme recognizes the target DNA sequence 5'CCGG and cleaves between the two cytosines to produce sticky ends with 5'CG overhangs
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of BgII: GCCNNNNNGGC. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of BglII: AGATCT. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
BbvCI cleaves the assymetric DNA sequence, 5'-CC-/-TCAGC-3'/5'-GC-/-TGAGG-3'. The R1 subunit of the enzyme acts at GC-/-TGAGG and the R2 subunit acts at CC-/-TCAGC. the DNA is cleaved initially in one strand, mainly that targeted by the R1 subunit. The other strand is then cleaved slowly by R2 before the enzyme dissociates from the DNA
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
cleavage at 5'-GGCGC-/-C-3. The enzyme displays an absolute requirement for two sites in close physical proximity, which are cleaved concertedly
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
cleavage of the DNA strand in DNA,RNA hybrids
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of Cfr10I: RCCGGY. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
Dactylococcus salina
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
ColE1 DNA
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
pBR322 DNA
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
SV40 DNA
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
cleavage of the DNA strand in DNA,RNA hybrids
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
lambda DNA
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
EcoRII cleaves DNA molecules with only a single recognition site or with very distant sites
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
Eco1524I recognizes the sequence 6-bp palindromic 5'AGG downward arrow CCT3', producing blunt end
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
EcoRII requires simultaneous binding of three rather than two recognition sites in cis to achieve concerted DNA cleavage at a single site
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
mechanochemical model of induced-fit reactions on DNA. Strongly decreased association rate is obtained on streched DNA
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
one metal ion and two water molecules are observed near the active site of the DNA complex. The metal ion is a Lewis acid that stabilizes the pentavalent phosphorus atom in the transition state. One water molecule, activated by Lys126, attacks the phosphorous atom in an SN2 mechanism, whereas the other water interacts with the 3'-leaving oxygen to donnate a proton to the oxygen
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of BstYI: GATATC. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of EcoO109I: RGGNCCY. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of EcoRI: GAATTC. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of EcoRII: CCWGG. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
EcoRII recognizes two units of recognition sequences (5'-CCWGG-3') included in one DNA chain (cis-binding) or in two DNA chains one by one (trans-binding), and cleaves either site
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
BstYI recognizes the degenerate sequence 5'-RGATCY-3' (where R is A/G and Y is C/T)
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of Bse634I: RCCGGY. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of BsoBI: CYCGRG. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of BstYI: RGATCY. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
BstF5I cleaves DNA 2 bases 3' to the recognition site on one strand and immediately 3' to the recognition site on the opposite strand, leaving a two base overhang
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
BstF5I cleaves DNA 2 bases 3' to the recognition site on one strand and immediately 3' to the recognition site on the opposite strand, leaving a two base overhang
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
cleavage of the DNA strand in DNA,RNA hybrids
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
cleavage of the DNA strand in DNA,RNA hybrids
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
HinP1I recognizes and cleaves a palindromic tetranucleotide sequence (G-/-CGC) in double-stranded DNA, producing 2 nt 5' overhanging ends
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of HincII: GTYRAC. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of HindIII: AAGCTT. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
HpyAXII effectively restricts both unmethylated plasmid and chromosomal DNA during natural transformation, the enzyme targets the tetramer 5'-GTAC-3'
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
R.Hpy188I recognizes the sequence TCNGA and cleaves between nucleotides N and G to generate a one-base 3' overhang
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
R.Hpy188I recognizes the sequence TCNGA and cleaves between nucleotides N and G to generate a one-base 3' overhang
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
R.KpnI cleaves the DNA sequence 5'-GGTAC-/-C-3', generating 3' four base overhangs
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
cleavage at 5'-G-/-GCGCC-3'. The enzyme cuts only one bond per turnover but acts at individual sites, preferring intact to nicked sites
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
Mva1269I restriction endonuclease recognizes an asymmetric DNA sequence 5'-GAATGCN-/-3'/5'-NG-/-CATTC-3' and cuts top and bottom DNA strands. The enzyme possesses two active sites responsible for the sequential cleavage of each DNA strand, which has evolved by fusion of a sequence specific nuclease domain, similar to EcoRI, to a nonspecific nuclease domain, similar to FokI
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of NaeI: GCCGGC. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
single strandede DNA and double stranded DNA
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
MmeI cuts DNA two turns of the helix away from its asymmetric recognition sequence, 5'-TCCRACN20/N18-3', MmeI modifies only the adenine in the top strand, 5'-TCCRAC-3', MmeI endonuclease activity is blocked by this top strand adenine methylation and is unaffected by methylation of the adenine in the complementary strand, 5'-GTYGGA-3', MmeI methylates its recognition site following DNA cleavage
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
cleavage at 5'-GG-/-CGCC-3'. The enzyme cuts both strands of its recognition sites, but shows full activity only when bound to two sites, which are then cleaved concertedly
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of MspI: CCGG. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of MunI: CAATTG. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of NgoMIV: GCCGGC. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
Neisseria mucosa heidelbergensis
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
cleavage at 5'-GG-/-CGCC-3'. The enzyme binds two sites, but cleaves only one bond per DNA-binding event
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
cleavage at 5'-GGC-/-GCC-3'. The enzyme cuts both strands at individual sites
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of FokI: GGATG. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
the FokI enzyme cleaves DNA 9 bases 3' to the recognition site on one strand and 13 bases from the recognition site on the other strand, leaving a four base overhang protruding 5' end
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
cleavage at 5'-GGC-/-GCC-3'. The enzyme cuts both strands at individual sites
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of PvuII: CAGCTG. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
the enzyme recognizes 5'-GTAC and leaves a 3'-TA overhang (5'-GTA/C)
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
cleaves DNA before the first C in the sequence 5'-CCWGG3'. W is A or T
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
cleavage at 5'-GGC-/-GCC-3'. The enzyme cuts both strands at individual sites
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
cleavage of the DNA strand in DNA,RNA hybrids
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA containing 5-fluoro-dC + H2O
?
perfectly hydrolyzes the DNA containing F5dC
-
-
?
DNA containing 5-fluoro-dC + H2O
?
perfectly hydrolyzes the DNA containing F5dC
-
-
?
DNA containing 5-fluoro-dC + H2O
?
perfectly hydrolyzes the DNA containing F5dC
-
-
?
DNA containing 5-fluoro-dC + H2O
?
perfectly hydrolyzes the DNA containing F5dC
-
-
?
DNA containing 5-fluoro-dC + H2O
?
perfectly hydrolyzes the DNA containing F5dC
-
-
?
DNA containing 5-fluoro-dC + H2O
?
perfectly hydrolyzes the DNA containing F5dC
-
-
?
DNA containing 5-fluoro-dC + H2O
?
Fuscovulum blasticum
perfectly hydrolyzes the DNA containing 5-fluoro-dC
-
-
?
DNA containing 5-fluoro-dC + H2O
?
perfectly hydrolyzes the DNA containing 5-fluoro-dC
-
-
?
DNA containing 5-fluoro-dC + H2O
?
perfectly hydrolyzes the DNA containing F5dC
-
-
?
DNA containing 5-fluoro-dC + H2O
?
perfectly hydrolyzes the DNA containing F5dC
-
-
?
DNA containing 5-fluoro-dC + H2O
?
perfectly hydrolyzes the DNA containing F5dC
-
-
?
DNA containing 5-fluoro-dC + H2O
?
perfectly hydrolyzes the DNA containing 5-fluoro-dC
-
-
?
DNA containing 5-fluoro-dC + H2O
?
perfectly hydrolyzes the DNA containing 5-fluoro-dC
-
-
?
DNA containing 5-fluoro-dC + H2O
?
perfectly hydrolyzes the DNA containing 5-fluoro-dC
-
-
?
DNA containing 5-fluoro-dC + H2O
?
perfectly hydrolyzes the DNA containing 5-fluoro-dC
-
-
?
DNA containing 5-fluoro-dU + H2O
?
perfectly hydrolyzes the DNA containing F5dU
-
-
?
DNA containing 5-fluoro-dU + H2O
?
perfectly hydrolyzes the DNA containing F5dU
-
-
?
DNA containing 5-fluoro-dU + H2O
?
perfectly hydrolyzes the DNA containing F5dU
-
-
?
DNA containing 5-fluoro-dU + H2O
?
hydrolyzes the DNA containing F5dU
-
-
?
DNA containing 5-fluoro-dU + H2O
?
hydrolyzes the DNA containing F5dU
-
-
?
DNA containing 5-fluoro-dU + H2O
?
hydrolyzes the DNA containing F5dU
-
-
?
DNA containing 5-fluoro-dU + H2O
?
Fuscovulum blasticum
perfectly hydrolyzes the DNA containing 5-fluoro-dU
-
-
?
DNA containing 5-fluoro-dU + H2O
?
perfectly hydrolyzes the DNA containing 5-fluoro-dU
-
-
?
DNA containing 5-fluoro-dU + H2O
?
perfectly hydrolyzes the DNA containing F5dU
-
-
?
DNA containing 5-fluoro-dU + H2O
?
perfectly hydrolyzes the DNA containing F5dU
-
-
?
DNA containing 5-fluoro-dU + H2O
?
perfectly hydrolyzes the DNA containing F5dU
-
-
?
DNA containing 5-fluoro-dU + H2O
?
perfectly hydrolyzes the DNA containing 5-fluoro-dU
-
-
?
DNA containing 5-fluoro-dU + H2O
?
perfectly hydrolyzes the DNA containing 5-fluoro-dU
-
-
?
DNA containing 5-fluoro-dU + H2O
?
hydrolyzes the DNA containing 5-fluoro-dU
-
-
?
DNA containing 5-methyl-dC + H2O
?
hydrolyzes the DNA containing F5dC
-
-
?
DNA containing 5-methyl-dC + H2O
?
Fuscovulum blasticum
perfectly hydrolyzes the DNA containing 5-fluoro-dC
-
-
?
DNA containing 5-methyl-dC + H2O
?
hydrolyzes the DNA containing F5dC
-
-
?
DNA containing 5-methyl-dC + H2O
?
hydrolyzes the DNA containing F5dC
-
-
?
DNA containing 5-methyl-dC + H2O
?
perfectly hydrolyzes the DNA containing F5dC
-
-
?
DNA containing 5-methyl-dC + H2O
?
perfectly hydrolyzes the DNA containing 5-methyl-dC
-
-
?
DNA containing 5-methyl-dC + H2O
?
perfectly hydrolyzes the DNA containing 5-methyl-dC
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
the enzyme recognizes the sequence 5'-Pu*CCGGPy and cleaves it as indicated by the star
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
cleavage by EcoRI is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GAATTC
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
cleavage by EcoRV is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GATATC
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
EcoRI recognizes 5'-GAATTC-3' while EcoRV recognizes 5'-GATATC-3', leaving overhangs and blunt DNA segments, respectively
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
cleavage by HindII is blunt, producing fragments with flush ends
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
LmoJ2 recognizes GCWGC (W is A or T)
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
LmoJ3 recognizes GCNGC
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
LmoJ2 recognizes GCWGC (W is A or T)
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
LmoJ3 recognizes GCNGC
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
double-stranded DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
dsDNA + H2O
?
-
recognition sequence is 5-GGACC-3/3-CCTGG-5, enzyme cleaves between the guanosin residues at both strands
sticky end fragments
-
?
dsDNA + H2O
?
-
recognition sequence is 5-GCGC-3/3-CGCG-5, enzyme cleaves in the middle of the tetranucleotide sequence
blunt end fragments
-
?
dsDNA + H2O
?
-
recognition sequence is 5-GCGC-3/3-CGCG-5, enzyme cleaves in the middle of the tetranucleotide sequence
blunt end fragments
-
?
dsDNA + H2O
?
-
recognition sequence is 5-GGACC-3/3-CCTGG-5, enzyme cleaves between the guanosin residues at both strands
sticky end fragments
-
?
pT7Blue plasmid + H2O
?
-
the enzyme partially digests the CATG site within the pT7Blue plasmid. The digestion efficiency of UpaP162 depends on the sequences around CATG motifs
-
-
?
pT7Blue plasmid + H2O
?
Ureaplasma parvum OMC-P162
-
the enzyme partially digests the CATG site within the pT7Blue plasmid. The digestion efficiency of UpaP162 depends on the sequences around CATG motifs
-
-
?
additional information
?
-
no hydrolysis of DNA containing 5-methyl-dC
-
-
?
additional information
?
-
no hydrolysis of DNA containing 5-methyl-dC
-
-
?
additional information
?
-
-
the enzyme has the recognition sequence (10/12) GCAN6TGC (12/10), of which it needs 2 on the substrate to be active. It excises 32 bp, and requires S-adenosyl-L-methionine
-
-
?
additional information
?
-
-
the enzyme has the recognition sequence (7/12) GAACN6TCC (12/7), of which it needs 2 on the substrate to be active. It excises 27 bp, and does not require S-adenosyl-L-methionine
-
-
?
additional information
?
-
no hydrolysis of DNA containing 5-methyl-dC
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?
additional information
?
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the enzyme has the recognition sequence (8/13) GAGN5CTC (13/8), of which it needs 1 on the substrate to be active. It excises 27 bp, and requires S-adenosyl-L-methionine
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?
additional information
?
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no hydrolysis of DNA containing 5-methyl-dC
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?
additional information
?
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the enzyme has the recognition sequence (8/14) CCAN6GT (15/9): It excises 28 bp, and requires S-adenosyl-L-methionine
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?
additional information
?
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the enzyme has the recognition sequence (11/13) CAAN5GTGG (12/10), of which it needs 2 on the substrate to be active. It excises 33 bp, and requires S-adenosyl-L-methionine
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?
additional information
?
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the enzyme has the recognition sequence (11/13) CAAN5GTGG (12/10), of which it needs 2 on the substrate to be active. It excises 33 bp, and requires S-adenosyl-L-methionine
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?
additional information
?
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no hydrolysis of DNA containing 5-methyl-dC
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?
additional information
?
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no hydrolysis of DNA containing 5-methyl-dC
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?
additional information
?
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restriction endonuclease activity and modification methylase activity occur as separate proteins
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?
additional information
?
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the REBASE database contains information about recognition sites and cleavage sites
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?
additional information
?
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no activity is observed using 1-site DNA as substrate
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?
additional information
?
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schematic view of the hydrogen-bond interactions of the DNA with each subunit of the protein for the 2TA and 1TA complexes, overview
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?
additional information
?
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development of a self-cleavage assay to measure EcoRV-DNA competitive binding and to evaluate the influence of water activity, pH and salt concentration on the DNA substrate binding stringency of the enzyme in the absence of divalent ions. The enzyme can readily distinguish specific and nonspecific sequences. The relative specific-nonspecific binding constant increases strongly with increasing neutral solute concentration and with decreasing pH. In addition to divalent ions, water activity and pH are key parameters that strongly modulate binding specificity of EcoRV
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?
additional information
?
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EcoRV utilizes intersegmental hopping to a greater extent than does EcoRI
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?
additional information
?
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the enzyme has the recognition sequence (9/12) ACN5CTCC (10/7), of which it needs 2 on the substrate to be active. It excises 27 bp, and requires S-adenosyl-L-methionine
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?
additional information
?
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no hydrolysis of DNA containing 5-methyl-dC
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?
additional information
?
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the enzyme has the recognition sequence (7/13) GAYN5RTC (14/9). It excises 27 bp, and does not require S-adenosyl-L-methionine
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?
additional information
?
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restriction endonuclease activity and modification methylase activity occur as separate proteins
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?
additional information
?
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MvaI restriction endonuclease cuts 5'-CC-/-AGG-3'/5'-CC-/-TGG-3' sites. N4-methylation of the inner cytosines, Cm4CAGG/Cm4CTGG, protects the site against MvaI cleavage. MvaI nicks the G-strand of the related sequence (CCGGG/CCCGG, BcnI site) if the inner cytosines are C5-methylated: Cm5C-/-GGG/CCm5CGG. At M.SssI-methylated SmaI sites, of M.SssI DNA methyltransferase, where two oppositely oriented methylated BcnI sites partially overlap, double-nicking leads to double-strand cleavage (CCm5C-/-GGG/CCm5C-/-GGG) generating fragments with blunt ends
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?
additional information
?
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the enzyme has the recognition sequence (10/15) ACN4GTAYC (12/7), of which it needs 2 on the substrate to be active. It excises 28 bp, and requires S-adenosyl-L-methionine
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?
additional information
?
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usage of a plasmid containing a single BspRI recognition site to analyze kinetically nicking and second-strand cleavage under steady-state conditions. Cleavage of the supercoiled plasmid goes through a relaxed intermediate indicating sequential hydrolysis of the two strands. BspRI cleaves the two DNA strands sequentially
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?
additional information
?
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the head-to-head configuration substrate, pUC19HH1, is digested both as closed circular DNA and linear DNA
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?
additional information
?
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recognition site is 5-GAAGA-3, cleavage occurs 7 or 8 bp downstream and generates a single 3-protruding nucleotide
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?
additional information
?
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recognition site is 5-GAAGA-3, cleavage occurs 7 or 8 bp downstream and generates a single 3-protruding nucleotide
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?
additional information
?
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the enzyme has the recognition sequence (12/7) RCCGGY (7/12), of which it needs 2 on the substrate to be active. It excises 20 bp, and does not require S-adenosyl-L-methionine
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?
additional information
?
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BtsI recognizes and digests at GCAGTG(2/0)
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?
additional information
?
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restriction endonuclease activity and modification methylase activity occur as separate proteins
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?
additional information
?
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no hydrolysis DNA containing 5-methyl-dC or 5-fluoro-dU
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?
additional information
?
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the enzyme has the recognition sequence (7/12) GAACN6CTC (13/8), of which it needs 2 on the substrate to be active. It excises 28 bp, and does not require S-adenosyl-L-methionine
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?
additional information
?
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no hydrolysis DNA containing 5-methy-dC
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?
additional information
?
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the enzyme does not cut Sulfolobus acidocaldarius DNA, as the recognition sequence GGCC in this DNA contains modified nucleotides
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?
additional information
?
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the enzyme recognizes tetranucleotide GGCC and cleaves DNA in the center of this sequence. DNA of Sulfolobus acidocaldarius is not cleaved by the enzyme
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?
additional information
?
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TatI recognition site is 5-AGTACA-3, the enzyme cleaves between first and second nucleotides generating 5-ends protruding four bases, TauI recognition site is 5-GCGSGC-3, the enzyme cleaves between fourth and fifth nucleotides generating 3-ends protruding three bases
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?
additional information
?
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the enzyme has the recognition sequence (10/12) CGAN6TGC (12/10), of which it needs 2 on the substrate to be active. It excises 32 bp, and requires S-adenosyl-L-methionine
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?
