3.1.21.4: type II site-specific deoxyribonuclease
This is an abbreviated version!
For detailed information about type II site-specific deoxyribonuclease, go to the full flat file.
Reaction
endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
=
Synonyms
AatII, AcuI, acuIRM, AfeI, AflII, AflIII, aflIIR, AgeI, AhaIII, AlfI, AloI, AluI, AlwNI, Apa BI, Apa CI, Apa DI, ApaI, ApaLI, apaLIR, AsuI, AsuII, AvaI, AvaII, AvaIII, AvrII, BaeI, BamHI, bamHIR, BbeI, BbvCI, BbvI, BbvII, bbvIR, BcefI, BcgI, BclI, Bfi2411I, BgII, BglI, BglII, bglIIR, BinI, BmrI, BplI, BpmI, bpmIR, BpuEI, BpuIOI, BsaAI, BsaBI, BsaWI, BsaXI, Bse634I, BsePI, BseRI, BseRI endonuclease, BsgI, BsmAI, BsmBI, BsmI, BsoBI, Bsp CI, BspD6I, BspGI, BspHI, BspI, BspMI, BSPMII, BspRI, BsrI, BssSI, BstEII, BstF5I, BstII, BstXI, BstYI, Bsu2413I, BtsI, CauII, Cfr101, Cfr10I, CfrBI, CfrI, CjeI, ClaI, CspCI, CstMI, CviAI, CviJI, DdeI, DNA restriction endonuclease, DNA restriction enzyme, DpnI, DpnII, DraII, DraIII, DrdI, DrdII, DsaI, EC 3.1.23, EC 3.1.24, EciI, Eco1524I, Eco311, Eco31I, Eco47III, Eco571, Eco57I, eco57IR, EcoO109I, EcoP15I, ecoP15Ires, EcoRI, EcoRII, ecoRIR, EcoRV, EcoVIII, EgeI, EheI, Endonuclease AbrI, Endonuclease AccI, Endonuclease AgeI, Endonuclease ApaLI, Endonuclease AvaI, Endonuclease BamHI, Endonuclease BanI, Endonuclease BglI, Endonuclease BglII, Endonuclease BsoBI, Endonuclease Bsp6I, Endonuclease BstVI, Endonuclease BsuBI, Endonuclease BsuFI, Endonuclease BsuRI, Endonuclease CeqI, Endonuclease Cfr10I, Endonuclease Cfr9I, Endonuclease CfrBI, Endonuclease CviAII, Endonuclease CviJI, Endonuclease DdeI, Endonuclease DpnI, Endonuclease DpnII, Endonuclease Eco47I, Endonuclease Eco47II, Endonuclease EcoRI, Endonuclease EcoRII, Endonuclease EcoRV, Endonuclease FokI, Endonuclease HaeII, Endonuclease HaeIII, Endonuclease HgAI, Endonuclease HgiBI, Endonuclease HgiCI, Endonuclease HgiCII, Endonuclease HgiDI, Endonuclease HgiEI, Endonuclease HgiGI, Endonuclease HhaII, Endonuclease HincII, Endonuclease HindII, Endonuclease HindIII, Endonuclease HindVP, Endonuclease HinfI, Endonuclease HpaI, Endonuclease HpaII, Endonuclease HphI, Endonuclease KpnI, Endonuclease LlaDCHI, Endonuclease MamI, Endonuclease MboI, Endonuclease MboII, Endonuclease MjaI, Endonuclease MjaII, Endonuclease MjaIII, Endonuclease MjaIV, Endonuclease MjaV, Endonuclease MjaVIP, Endonuclease MspI, Endonuclease MthTI, Endonuclease MthZI, Endonuclease MunI, Endonuclease MwoI, Endonuclease NaeI, Endonuclease NgoBI, Endonuclease NgoBV, Endonuclease NgoFVII, Endonuclease NgoMIV, Endonuclease NgoPII, Endonuclease NlaIII, Endonuclease NlaIV, Endonuclease NmeDIP, Endonuclease NspV, Endonuclease PaeR7I, Endonuclease PstI, Endonuclease PvuI, Endonuclease PvuII, Endonuclease RsrI, Endonuclease SacI, Endonuclease SalI, Endonuclease Sau3AI, Endonuclease Sau96I, Endonuclease ScaI, Endonuclease ScrFI, Endonuclease SfiI, Endonuclease SinI, Endonuclease SmaI, Endonuclease SsoII, Endonuclease StsI, Endonuclease TaqI, Endonuclease TthHB8I, Endonuclease XamI, Endonuclease XcyI, endoR*Bsp, Esp3I, EspI, FauI, FinI, Fnu4HI, FnuSII, FokI, fokIR, FseI, GdiII, GGCC-specific restriction endonuclease, GsuI, HaeI, HaeII, HaeIII, HaeVI, HgaI, HgiAI, HgiCI, HgiEI, HgiEII, HgiJII, HhaI, HhaII, HincII, HindII, HindIII, hindIIIR, HinfI, HinP1I, HpaI, HpaII, HphI, Hpy188I, Hpy8I, Hpy99II, Hpy99IV, Hpy99VIIIP, HpyAXII, HpyF17I, KasI, KpnI, KpnIR, Ksp6321, LlaDI, LlaII, LmoJ2, LmoJ3, MaeI, MaeII, MaeIII, MboI, MboII, McaTI, McrI, MfeI, MjaIV, MjaV, MluI, Mly113I, MlyI, MmeI, mmeIRM, MmoSTI, MnlI, More, MseI, MspA1I, MspI, MstI, MunI, Mva1269I, Mva1269I restriction endonuclease, MvaI, MwoI, NaeI, NarI, NcoI, ncoIR, NdeI, NgoMIV, NheI, NlaIII, NlaIV, NmeDI, NmeDIP, NotI, NruI, NspBII, NspV, Nt.BspD6I, nuclease, deoxyribonucleic restriction endo-, nuclease, restriction endodeoxyribo-, PabI, PflMI, PhoI, PleI, PmaCI, PPII, PpuMI, PsasM2I, PshAI, PspGI, PstI, pstIR, PvuI, PvuII, R.AbrI, R.AccI, R.AgeI, R.ApaLI, R.AvaI, R.BamHI, R.BanI, R.BglI, R.BglII, R.BsoBI, R.Bsp6I, R.BstVI, R.BsuBI, R.BsuFI, R.BsuRI, R.CeqI, R.Cfr10I, R.Cfr9I, R.CfrBI, R.CviAII, R.CviJI, R.DdeI, R.DpnI, R.DpnII, R.Eco47I, R.Eco47II, R.EcoRI, R.EcoRII, R.EcoRV, R.FokI, R.HaeII, R.HaeIII, R.HgAI, R.HgiBI, R.HgiCI, R.HgiCII, R.HgiDI, R.HgiEI, R.HgiGI, R.HhaII, R.HincII, R.HindII, R.HindIII, R.HindVP, R.HinfI, R.HpaI, R.HpaII, R.HphI, R.KpnI, R.LlaDCHI, R.MamI, R.MboI, R.MboII, R.MjaI, R.MjaII, R.MjaIII, R.MjaIV, R.MjaV, R.MjaVIP, R.MspI, R.MthTI, R.MthZI, R.MunI, R.MwoI, R.NaeI, R.NgoBI, R.NgoBV, R.NgoFVII, R.NgoI, R.NgoMIV, R.NgoPII, R.NgoV, R.NgoVII, R.NlaIII, R.NlaIV, R.NmeDIP, R.NspV, R.PaeR7I, R.PstI, R.PvuI, R.PvuII, R.RsrI, R.SacI, R.SalI, R.Sau3AI, R.Sau96I, R.ScaI, R.ScrFI, R.SfiI, R.SinI, R.SmaI, R.SsoII, R.StsI, R.TaqI, R.TthHB8I, R.XamI, R.XcyI, REase, restriction endodeoxyribonuclease, restriction endonuclease, restriction endonuclease EcoRV, restriction endonuclease SuaI, restriction enzyme, RleAI, RsaI, SacI, SacII, SacIR, SalI, Sau3AI, sau96I, SauI, ScaI, ScrFI, SduI, SecI, SfaNI, SfeI, SfiI, SfoI, SgrAI, Sho27844P, site-specific endonuclease BME142I, site-specific endonuclease Bme216I, site-specific endonuclease BmeI, site-specific type II endonuclease, SmaI, SmuI, SnaBI, SnaI, SpeI, SphI, SpiI, ss.BspD6I, Sse9I, SspI, StuI, StyI, SuaI, SuiI, TaqI, TaqII, TfiI, Tsp451, TspEI, TstI, Tth111I, Tth111II, type II ENase, type II REase, type II restriction endonuclease, type II restriction endonuclease EcoO109I, type II restriction enonuclease, type II restriction enzyme, type II restriction-modification protein, type II restriction-modification system, type II RM system, type IIB restriction endonuclease, type IIE restriction endonuclease, type IIF restriction endonuclease, type IIP REase, type IIP restriction endonuclease, Type IIS REase, type IIS restriction endonuclease, type IIS restriction enzyme, type IIT restriction endonuclease BbvCI, typeIIS restriction endonuclease, Uba1105I, Uba1108I, UbaLAI, UpaP162, UPV_230, VspI, XbaI, XcmI, XhoI, XhoII, XmaIII, XmnI
ECTree
Application
Application on EC 3.1.21.4 - type II site-specific deoxyribonuclease
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
analysis
-
the enzyme can be used in DNA-based diagnostic applications
analysis
-
method for following the digestion of DNA by restriction endonucleases in real time without the use of any extrinsic dyes or labels via linear dichroism spectroscopy
analysis
-
method for following the digestion of DNA by restriction endonucleases in real time without the use of any extrinsic dyes or labels via linear dichroism spectroscopy
analysis
-
method for following the digestion of DNA by restriction endonucleases in real time without the use of any extrinsic dyes or labels via linear dichroism spectroscopy
analysis
-
method for following the digestion of DNA by restriction endonucleases in real time without the use of any extrinsic dyes or labels via linear dichroism spectroscopy
analysis
-
method for following the digestion of DNA by restriction endonucleases in real time without the use of any extrinsic dyes or labels via linear dichroism spectroscopy
analysis
-
method for following the digestion of DNA by restriction endonucleases in real time without the use of any extrinsic dyes or labels via linear dichroism spectroscopy
analysis
-
genetic analysis of 24 Hungarian canine parvovirus strains collected from 2004 to 2008 revealed that all of them are type 2a strains. Due to a seemingly constant point mutation present in most of the Hungarian canine parvovirus 2a strains, a previously described MboII-based rapid identification of CPV2c strains unfortunately cannot be reliably used any more
analysis
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
analysis
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
analysis
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
analysis
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
analysis
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
analysis
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
analysis
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
analysis
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
analysis
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
analysis
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
analysis
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
-
biotechnology
-
evolvement of mutant enzymes with altered DNA cleavage specificities by application of an in vivo positive and negative selection system that applies evolutionary pressure either to favor the cleavage of a desired target sequence or to disfavor the cleavage of a nontarget sequence
biotechnology
-
generation of cleavage specificities of restriction endonucleases by swapping putative target recognition domains between the type IIB enzymes AloI, PpiI from Pseudomonas putida, and TstI from Thermus scotoductus. Individual target recognition domains recognize distinct parts of the bipartite DNA targets of these enzymes and are interchangeable. Engineering of a functional type IIB restriction endonuclease having previously undescribed DNA specificity and application in generation of type II enzymes with predetermined specificity
biotechnology
-
generation of cleavage specificities of restriction endonucleases by swapping putative target recognition domains between the type IIB enzymes AloI, PpiI from Pseudomonas putida, and TstI from Thermus scotoductus. Individual target recognition domains recognize distinct parts of the bipartite DNA targets of these enzymes and are interchangeable. Engineering of a functional type IIB restriction endonuclease having previously undescribed DNA specificity and application in generation of type II enzymes with predetermined specificity
biotechnology
-
generation of cleavage specificities of restriction endonucleases by swapping putative target recognition domains between the type IIB enzymes AloI, PpiI from Pseudomonas putida, and TstI from Thermus scotoductus. Individual target recognition domains recognize distinct parts of the bipartite DNA targets of these enzymes and are interchangeable. Engineering of a functional type IIB restriction endonuclease having previously undescribed DNA specificity and application in generation of type II enzymes with predetermined specificity
biotechnology
-
generation of cleavage specificities of restriction endonucleases by swapping putative target recognition domains between the type IIB enzymes AloI, PpiI from Pseudomonas putida, and TstI from Thermus scotoductus. Individual target recognition domains recognize distinct parts of the bipartite DNA targets of these enzymes and are interchangeable. Engineering of a functional type IIB restriction endonuclease having previously undescribed DNA specificity and application in generation of type II enzymes with predetermined specificity
-
molecular biology
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
molecular biology
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
molecular biology
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
molecular biology
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
molecular biology
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
molecular biology
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
molecular biology
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
molecular biology
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
molecular biology
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
molecular biology
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
molecular biology
tools for the dissection, analysis and reconstruction of DNA
molecular biology
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for future studies of large sets of restriction endonucleases and their activity
molecular biology
-
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity
molecular biology
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity
molecular biology
-
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity
molecular biology
-
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity
molecular biology
-
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity
molecular biology
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity
molecular biology
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity
molecular biology
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity
molecular biology
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity
molecular biology
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity
molecular biology
protein tagging with a wide variety of epitopes and/or fusion partners is used routinely to dissect protein function molecularly. Frequently the required DNA subcloning is inefficient, especially in cases where multiple constructs are desired for a given protein with unique tags. The generated clones have unwanted junction sequences introduced. To add versatile tags into the extracellular domain of the transmembrane protein THSD1, a protein tagging technique is developed that utilizes non-classical type IIS restriction enzymes that recognize non-palindromic DNA sequences and cleave outside of their recognition sites. The method is highly efficient and can precisely fuse any tag into any position of a protein in a scarless manner. IT is cost-efficient and adaptable because it uses commercially available type IIS restriction enzymes and is compatible with the traditional cloning system used by many labs
molecular biology
site-directed mutagenesis methods are very important in modern molecular biology, biochemistry, and protein engineering. A site-directed mutagenesis method that can be used for multiple mutation generation using type IIs restriction enzymes. This approach is faster and more convenient than the overlap polymerase chain reaction method due to its having fewer reaction steps and being cheaper than, but as convenient as, enzymatic assembly
molecular biology
-
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity
-
molecular biology
-
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity
-
molecular biology
-
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity
-
molecular biology
-
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity
-
molecular biology
-
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity
-
molecular biology
-
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA
-