# 3.1.21.4: type II site-specific deoxyribonuclease

This is an abbreviated version, for detailed information about type II site-specific deoxyribonuclease, go to the full flat file.

### Reaction

endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates =

### Synonyms

AatII, AflII, AflIII, AgeI, AhaIII, AlfI, AloI, AluI, AlwNI, Apa BI, Apa CI, Apa DI, ApaI, ApaLI, AsuI, AsuII, AvaI, AvaII, AvaIII, AvrII, BaeI, BamHI, BbeI, BbvCI, BbvI, BbvII, BcefI, BcgI, BclI, Bfi2411I, BgII, BglI, BglII, BinI, BmrI, BplI, BpuIOI, BsaAI, BsaBI, BsaXI, Bse634I, BsePI, BsmAI, BsmI, BsoBI, Bsp CI, BspD6I, BspGI, BspHI, BspI, BspMI, BSPMII, BspRI, BsrI, BssSI, BstEII, BstF5I, BstII, BstXI, BstYI, Bsu2413I, BtsI, CauII, Cfr101, Cfr10I, CfrBI, CfrI, CjeI, ClaI, CspCI, CstMI, CviAI, CviJI, DdeI, DNA restriction endonuclease, DNA restriction enzyme, DpnI, DpnII, DraII, DraIII, DrdI, DrdII, DsaI, EC 3.1.23, EC 3.1.24, EciI, Eco1524I, Eco311, Eco31I, Eco47III, Eco571, EcoO109I, EcoRI, EcoRII, EcoRV, EcoVIII, EgeI, EheI, Endonuclease AbrI, Endonuclease AccI, Endonuclease AgeI, Endonuclease ApaLI, Endonuclease AvaI, Endonuclease BamHI, Endonuclease BanI, Endonuclease BglI, Endonuclease BglII, Endonuclease BsoBI, Endonuclease Bsp6I, Endonuclease BstVI, Endonuclease BsuBI, Endonuclease BsuFI, Endonuclease BsuRI, Endonuclease CeqI, Endonuclease Cfr10I, Endonuclease Cfr9I, Endonuclease CfrBI, Endonuclease CviAII, Endonuclease CviJI, Endonuclease DdeI, Endonuclease DpnI, Endonuclease DpnII, Endonuclease Eco47I, Endonuclease Eco47II, Endonuclease EcoRI, Endonuclease EcoRII, Endonuclease EcoRV, Endonuclease FokI, Endonuclease HaeII, Endonuclease HaeIII, Endonuclease HgAI, Endonuclease HgiBI, Endonuclease HgiCI, Endonuclease HgiCII, Endonuclease HgiDI, Endonuclease HgiEI, Endonuclease HgiGI, Endonuclease HhaII, Endonuclease HincII, Endonuclease HindII, Endonuclease HindIII, Endonuclease HindVP, Endonuclease HinfI, Endonuclease HpaI, Endonuclease HpaII, Endonuclease HphI, Endonuclease KpnI, Endonuclease LlaDCHI, Endonuclease MamI, Endonuclease MboI, Endonuclease MboII, Endonuclease MjaI, Endonuclease MjaII, Endonuclease MjaIII, Endonuclease MjaIV, Endonuclease MjaV, Endonuclease MjaVIP, Endonuclease MspI, Endonuclease MthTI, Endonuclease MthZI, Endonuclease MunI, Endonuclease MwoI, Endonuclease NaeI, Endonuclease NgoBI, Endonuclease NgoBV, Endonuclease NgoFVII, Endonuclease NgoMIV, Endonuclease NgoPII, Endonuclease NlaIII, Endonuclease NlaIV, Endonuclease NmeDIP, Endonuclease NspV, Endonuclease PaeR7I, Endonuclease PstI, Endonuclease PvuI, Endonuclease PvuII, Endonuclease RsrI, Endonuclease SacI, Endonuclease SalI, Endonuclease Sau3AI, Endonuclease Sau96I, Endonuclease ScaI, Endonuclease ScrFI, Endonuclease SfiI, Endonuclease SinI, Endonuclease SmaI, Endonuclease SsoII, Endonuclease StsI, Endonuclease TaqI, Endonuclease TthHB8I, Endonuclease XamI, Endonuclease XcyI, endoR*Bsp, Esp3I, EspI, FauI, FinI, Fnu4HI, FnuSII, FokI, FseI, GdiII, GGCC-specific restriction endonuclease, GsuI, HaeI, HaeII, HaeIII, HaeVI, HgaI, HgiAI, HgiCI, HgiEI, HgiEII, HgiJII, HhaI, HhaII, HincII, HindII, HindIII, HinfI, HinP1I, HpaI, HpaII, HphI, Hpy188I, Hpy8I, Hpy99II, Hpy99IV, Hpy99VIIIP, HpyAXII, HpyF17I, KasI, KpnI, Ksp6321, LlaDI, LlaII, LmoJ2, LmoJ3, MaeI, MaeII, MaeIII, MboI, MboII, McaTI, McrI, MfeI, MjaIV, MjaV, MluI, Mly113I, MlyI, MmeI, MnlI, More, MseI, MspA1I, MspI, MstI, MunI, Mva1269I, Mva1269I restriction endonuclease, MvaI, MwoI, NaeI, NarI, NcoI, NdeI, NgoMIV, NheI, NlaIII, NlaIV, NmeDI, NmeDIP, NotI, NruI, NspBII, NspV, Nt.BspD6I, nuclease, deoxyribonucleic restriction endo-, nuclease, restriction endodeoxyribo-, PabI, PflMI, PhoI, PleI, PmaCI, PPII, PpuMI, PsasM2I, PshAI, PspGI, PstI, PvuI, PvuII, R.AbrI, R.AccI, R.AgeI, R.ApaLI, R.AvaI, R.BamHI, R.BanI, R.BglI, R.BglII, R.BsoBI, R.Bsp6I, R.BstVI, R.BsuBI, R.BsuFI, R.BsuRI, R.CeqI, R.Cfr10I, R.Cfr9I, R.CfrBI, R.CviAII, R.CviJI, R.DdeI, R.DpnI, R.DpnII, R.Eco47I, R.Eco47II, R.EcoRI, R.EcoRII, R.EcoRV, R.FokI, R.HaeII, R.HaeIII, R.HgAI, R.HgiBI, R.HgiCI, R.HgiCII, R.HgiDI, R.HgiEI, R.HgiGI, R.HhaII, R.HincII, R.HindII, R.HindIII, R.HindVP, R.HinfI, R.HpaI, R.HpaII, R.HphI, R.KpnI, R.LlaDCHI, R.MamI, R.MboI, R.MboII, R.MjaI, R.MjaII, R.MjaIII, R.MjaIV, R.MjaV, R.MjaVIP, R.MspI, R.MthTI, R.MthZI, R.MunI, R.MwoI, R.NaeI, R.NgoBI, R.NgoBV, R.NgoFVII, R.NgoI, R.NgoMIV, R.NgoPII, R.NgoV, R.NgoVII, R.NlaIII, R.NlaIV, R.NmeDIP, R.NspV, R.PaeR7I, R.PstI, R.PvuI, R.PvuII, R.RsrI, R.SacI, R.SalI, R.Sau3AI, R.Sau96I, R.ScaI, R.ScrFI, R.SfiI, R.SinI, R.SmaI, R.SsoII, R.StsI, R.TaqI, R.TthHB8I, R.XamI, R.XcyI, REase, restriction endodeoxyribonuclease, restriction endonuclease, restriction endonuclease EcoRV, restriction endonuclease SuaI, restriction enzyme, RleAI, RsaI, SacI, SacII, SalI, Sau3AI, sau96I, SauI, ScaI, ScrFI, SduI, SecI, SfaNI, SfeI, SfiI, SfoI, SgrAI, Sho27844P, site-specific endonuclease BME142I, site-specific endonuclease Bme216I, site-specific endonuclease BmeI, site-specific type II endonuclease, SmaI, SnaBI, SnaI, SpeI, SphI, SpiI, ss.BspD6I, Sse9I, SspI, StuI, StyI, SuaI, SuiI, TaqI, TaqII, TfiI, Tsp451, TspEI, TstI, Tth111I, Tth111II, type II ENase, type II REase, type II restriction endonuclease, type II restriction endonuclease EcoO109I, type II restriction enonuclease, type II restriction enzyme, type II restriction-modification protein, type II restriction-modification system, type II RM system, type IIB restriction endonuclease, type IIE restriction endonuclease, type IIP REase, type IIP restriction endonuclease, Type IIS REase, type IIS restriction endonuclease, type IIS restriction enzyme, typeIIS restriction endonuclease, Uba1105I, Uba1108I, VspI, XbaI, XcmI, XhoI, XhoII, XmaIII, XmnI