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3.1.21.3: type I site-specific deoxyribonuclease

This is an abbreviated version!
For detailed information about type I site-specific deoxyribonuclease, go to the full flat file.

Word Map on EC 3.1.21.3

Reaction

endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolysed =

Synonyms

adenosine triphosphate-dependent deoxyribonuclease, ATP-dependent deoxyribonuclease, ATP-dependent DNase, BsaHI restriction-modification system, C.PvuII, CfrAI, deoxyribonuclease (ATP- and S-adenosyl-L-methionine dependent), deoxyribonuclease (ATP-dependent), DNase, EC 3.1.23, EC 3.1.24, EC 3.1.4.33, Eco377I, Eco394I, Eco585I, Eco646I, Eco777I, Eco826I, Eco851I, Eco912I, EcoA0ORF42P, EcoAI, EcoAO83I, EcoB, EcoBI, EcoDI, EcoDXXI, EcoEI, EcoGIV, EcoK, EcoKI, EcoKI type I DNA restriction enzyme, EcoKI type I restriction-modification system, EcoprrI, EcoR, EcoR124, EcoR124/3I, EcoR124I, EcoR124II, EcoRII modification enzyme, EcoRII RM gene complex, EcoRII system, endodeoxyribonuclease, Esp1396I, exodeoxyribonuclease, H91_orf206, H91_orf376, HpyAXII restriction-modification system, HsdM, HsdR, hsdS, KpnBI, More, MpnORFDAP, MpnORFDBP, nuclease, deoxyribo-, nuclease, deoxyribo-, ATP-dependent, PspGI endonuclease, PspGI restriction-modification system, R. BsaHI, R.EcoAI, R.EcoEI, R.EcoKI, R.EcoR124I restriction endonuclease, R.EcoR124II, R.EcoR124INT, R.HpyAXII, R.PspGI, REase, RecoK, restriction-modification system, Sau1, Sau1 type I restriction-modification system, Sau1 Type I RM system, SauMW2I, SauMW2II, SauN315I, SauN315II, StyLTIII, StySBI, StySEAI, StySGI, StySJI, StySKI, StySPI, StySQI, type I R-M enzyme, type I R-M system, type I restriction enzyme, type I restriction modification enzyme, type I restriction-modification enzyme, type I restriction-modification system, type I restriction-modification system EcoR124I, type IB restriction enzyme, type II R-M system, type II restriction-modification system

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters
                3.1.21.3 type I site-specific deoxyribonuclease

Purification

Purification on EC 3.1.21.3 - type I site-specific deoxyribonuclease

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
C.Esp1396I-6His and C.Esp1396I without hexahistidine tag
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EcoR124I methylase is purified from Escherichia coli strain JM109(DE3) harboring plasmid pJS4M
enzyme EcoB
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enzyme EcoK
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expression in Escherichia coli
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HisTrap column chromatography, DEAE column chromatography, and heparin column chromatography
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HsdM subunit, on anion-exchange column, more than 95% pure
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HsdR fusion protein purified by gel filtration. HsdR with five additional amino acids (YFQGA) at the N-terminus purified on anion-exchange column and by gel filtration, purified protein is more than 95% pure
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mixture of two enzyme species, the larger species has the stoichiometry R2M2S1, the smaller species has the stoichiometry of R1M2S1.only the R2M2S1 complex is capable of DNA cleavage, the R1M2S1 complex retains ATPase activity
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Mtase(DELTA50)
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mutant methylase
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native HsdR protein homogeneously purified by sequential chromatographic steps
nickel-affinity column chromatography, ion-exchange chromatography, and gel filtration
pHluorin-assisted purification of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I
recombinant motor subunit HsdR of isoform EcoR124I
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wild-type R.PspGI and its mutant purified by chromatography, to homogeneity
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