3.1.21.3: type I site-specific deoxyribonuclease

This is an abbreviated version!
For detailed information about type I site-specific deoxyribonuclease, go to the full flat file.

Reaction

endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolysed =

Synonyms

adenosine triphosphate-dependent deoxyribonuclease, ATP-dependent deoxyribonuclease, ATP-dependent DNase, BsaHI restriction-modification system, C.PvuII, CfrAI, deoxyribonuclease (ATP- and S-adenosyl-L-methionine dependent), deoxyribonuclease (ATP-dependent), DNase, EC 3.1.23, EC 3.1.24, EC 3.1.4.33, Eco377I, Eco394I, Eco585I, Eco646I, Eco777I, Eco826I, Eco851I, Eco912I, EcoA0ORF42P, EcoAI, EcoAO83I, EcoB, EcoBI, EcoDI, EcoDXXI, EcoEI, EcoGIV, EcoK, EcoKI, EcoKI type I DNA restriction enzyme, EcoKI type I restriction-modification system, EcoprrI, EcoR, EcoR124, EcoR124/3I, EcoR124I, EcoR124II, EcoRII modification enzyme, EcoRII RM gene complex, EcoRII system, endodeoxyribonuclease, Esp1396I, exodeoxyribonuclease, H91_orf206, H91_orf376, HpyAXII restriction-modification system, HsdM, HsdR, hsdS, KpnBI, More, MpnORFDAP, MpnORFDBP, nuclease, deoxyribo-, nuclease, deoxyribo-, ATP-dependent, PspGI endonuclease, PspGI restriction-modification system, R. BsaHI, R.EcoAI, R.EcoEI, R.EcoKI, R.EcoR124I restriction endonuclease, R.EcoR124II, R.EcoR124INT, R.HpyAXII, R.PspGI, REase, RecoK, restriction-modification system, Sau1, Sau1 type I restriction-modification system, Sau1 Type I RM system, SauMW2I, SauMW2II, SauN315I, SauN315II, StyLTIII, StySBI, StySEAI, StySGI, StySJI, StySKI, StySPI, StySQI, type I R-M enzyme, type I R-M system, type I restriction enzyme, type I restriction modification enzyme, type I restriction-modification enzyme, type I restriction-modification system, type I restriction-modification system EcoR124I, type IB restriction enzyme, type II R-M system, type II restriction-modification system

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters

                3.1.21.3 type I site-specific deoxyribonuclease

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Results	in table
7	Activating Compound
36239	AA Sequence
5	Application
1	CAS Registry Number
27	Cloned(Commentary)
19	Cofactor
10	Crystallization (Commentary)
5	General Information
7	Inhibitors
2	KM Value [mM]
3	Localization
8	Metals/Ions
22	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
71	Organism
27	PDB ID
2	pH Optimum
1	Posttranslational Modification
28	Protein Variants
23	Purification (Commentary)
3	Reaction
1	Reaction Type
66	Reference
68	Substrates and Products (Substrate)
16	Subunits
181	Synonyms
1	Temperature Optimum [°C]
1	Turnover Number [1/s]

Natural Substrates Products

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of the Enzyme Summary Page.

Natural Substrates Products on EC 3.1.21.3 - type I site-specific deoxyribonuclease

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

DNA + H2O

?

2 entries

duplex DNA + ATP

double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate

2 entries

duplex DNA + ATP

double-stranded DNA fragments with terminal 5'-phosphate + ADP + phosphate

Vibrio vulnificus

Q7MPU6

-

728867

-

-

?

linear plasmid DNA pDRM-2R + ATP

?

Escherichia coli

P10486

-

730230

-

-

?

plasmid DNA + H2O

?

2 entries

supercoiled plasmid DNA pRK + ATP

?

Escherichia coli

P10486

-

730230

-

-

?

additional information

?

-

9 entries

DNA + H2O

?

Helicobacter pylori

-

R.HpyAXII effectively restricts chromosomal DNA during natural transformation

694457

-

-

?

DNA + H2O

?

Helicobacter pylori NSH57

-

R.HpyAXII effectively restricts chromosomal DNA during natural transformation

694457

-

-

?

duplex DNA + ATP

double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate

Escherichia coli

-

type I enzymes recognize bipartite DNA sequences comprising two half-sequences separated by a gap, for example, AACNNNNNNGTGC (AAC N6 GTGC) where N=any base

730505

-

-

?

duplex DNA + ATP

double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate

Staphylococcus aureus

-

-

730497

-

-

?

plasmid DNA + H2O

?

Helicobacter pylori

-

R.HpyAXII effectively restricts unmethylated plasmid during natural transformation

694457

-

-

?

plasmid DNA + H2O

?

Helicobacter pylori NSH57

-

R.HpyAXII effectively restricts unmethylated plasmid during natural transformation

694457

-

-

?

additional information

?

-

Escherichia coli

-

-

134250

-

-

?

additional information

?

-

Escherichia coli

-

a bacterial population may switch the recognition sequence of its type I restriction-modification system by single recombination events and thus is able to maintain a prokaryotic analogue of the immune system of variable specificity

134242

-

-

?

additional information

?

-

Escherichia coli

-

enzyme restricts the exchange of genetic material between bacteria of different strains or species

134249

-

-

?

additional information

?

-

Escherichia coli K-12

-

C.PvuII binding to operator left activates transcription. Binding preference for operator left over operator right. All four bases of the inter-operator TGTA spacer are required for C.PvuII-dependent activation

694471

-

-

?

additional information

?

-

Salmonella enterica subsp. enterica serovar Typhimurium

-

enzyme restricts the exchange of genetic material between bacteria of different strains or species

134249

-

-

?

additional information

?

-

Staphylococcus aureus

-

restricts unmodified phage DNA after both infection and transfection

134250

-

-

?

additional information

?

-

Staphylococcus aureus

-

isoform Sau1 is the major mechanism for blocking transfer of resistance genes and other mobile genetic elements into Staphylococcus aureus isolates from other species, as well as for controlling the spread of resistance genes between isolates of different Staphylococcus aureus lineages

680463

-

-

?

additional information

?

-

Staphylococcus aureus RN450

-

restricts unmodified phage DNA after both infection and transfection

134250

-

-

?

additional information

?

-

synthetic construct

-

basal transcription from promoter esp1396ICR results in gradual accumulation of C.Esp1396I, which upon dimerization binds to a single site in promoter esp1396IM, preventing further transcription from this promoter. Further accumulation of C.Esp1396I results in activation and then gradual repression of promoter esp1396ICR

700507

-

-

?