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3.1.21.3: type I site-specific deoxyribonuclease

This is an abbreviated version!
For detailed information about type I site-specific deoxyribonuclease, go to the full flat file.

Word Map on EC 3.1.21.3

Reaction

endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolysed =

Synonyms

adenosine triphosphate-dependent deoxyribonuclease, ATP-dependent deoxyribonuclease, ATP-dependent DNase, BsaHI restriction-modification system, C.PvuII, CfrAI, deoxyribonuclease (ATP- and S-adenosyl-L-methionine dependent), deoxyribonuclease (ATP-dependent), DNase, EC 3.1.23, EC 3.1.24, EC 3.1.4.33, Eco377I, Eco394I, Eco585I, Eco646I, Eco777I, Eco826I, Eco851I, Eco912I, EcoA0ORF42P, EcoAI, EcoAO83I, EcoB, EcoBI, EcoDI, EcoDXXI, EcoEI, EcoGIV, EcoK, EcoKI, EcoKI type I DNA restriction enzyme, EcoKI type I restriction-modification system, EcoprrI, EcoR, EcoR124, EcoR124/3I, EcoR124I, EcoR124II, EcoRII modification enzyme, EcoRII RM gene complex, EcoRII system, endodeoxyribonuclease, Esp1396I, exodeoxyribonuclease, H91_orf206, H91_orf376, HpyAXII restriction-modification system, HsdM, HsdR, hsdS, KpnBI, More, MpnORFDAP, MpnORFDBP, nuclease, deoxyribo-, nuclease, deoxyribo-, ATP-dependent, PspGI endonuclease, PspGI restriction-modification system, R. BsaHI, R.EcoAI, R.EcoEI, R.EcoKI, R.EcoR124I restriction endonuclease, R.EcoR124II, R.EcoR124INT, R.HpyAXII, R.PspGI, REase, RecoK, restriction-modification system, Sau1, Sau1 type I restriction-modification system, Sau1 Type I RM system, SauMW2I, SauMW2II, SauN315I, SauN315II, StyLTIII, StySBI, StySEAI, StySGI, StySJI, StySKI, StySPI, StySQI, type I R-M enzyme, type I R-M system, type I restriction enzyme, type I restriction modification enzyme, type I restriction-modification enzyme, type I restriction-modification system, type I restriction-modification system EcoR124I, type IB restriction enzyme, type II R-M system, type II restriction-modification system

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters
                3.1.21.3 type I site-specific deoxyribonuclease

Cloned

Cloned on EC 3.1.21.3 - type I site-specific deoxyribonuclease

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
248-bp insertion fragments of the ptypeI plasmid inserted at the HincII site of pUC19
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EcoR124I HsdR with selenomethionine labeling expressed in Escherichia coli
EcoR124I methylase is purified from Escherichia coli strain JM109(DE3) harboring plasmid pJS4M
enzyme with and without a temperature sensitive mutation in the hsdS gene are cloned in pBR322 plamid and introduced into Escherichia coli C3-6
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esp1396I genes expressed in Escherichia coli HB101 and XL1-Blue
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expressed in Escherichia coli B834 (DE3) cells
expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli BL21(DE3) Gold cells
expressed in Escherichia coli strains BNH670 or GM31 harboring a plasmid with various versions of the EcoRII RM gene complex
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expression in Escherichia coli
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full length HsdR transformed into Escherichia coli B834(DE3) cells
His-tagged gene cloned into the NheI/EcoRI sites of the pTXBI vector and expressed in Escherichia coli ER2566
hsdR PCR product cloned into pMAD, giving rise to pDELTAHsdR-1. Plasmid electroporated into the transformable strain RN4220 at 30°C, with erythromycin selection, and subsequently transduced to NCTC8325-4, SH1000 and COL using phage 80alpha
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HsdR subunit cloned into pProExHTc and expressed in Escherichia coli B834(DE3)
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HsdR subunit of isoform EcoR124I, expression in Escherichia coli
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of hsdS(DELTA50)
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PCR-amplified gene for C.PvuII cloned downstream of the arabinose-inducible PBAD promoter in vector pBAD24 yielding plasmids pIM1
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pCR-TOPO/hpyAXII digested with EcoRI and subcloned into plasmid Bluescript SK+. Plasmid pET15b/hpyAXIIR expressed in Escherichia coli strain BL21CodonPlus(DE3)-RIL
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pHluorin-assisted expression of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I
putative HsdM subunit amplified, cloned into pProExHTc, which expresses 25 extra amino acids containing six consecutive His residues at the N-terminus. The expression construct transformed into Escherichia coli B834 (DE3)
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recombinant plasmid pJS4M overproducing HsdM compared to HsdS
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transforming the Escherichia coli BL21(DE3) strains with a BAC C4/1 carrying the hsdR, hsdM and hsdS genes of EcoAO83I and with plasmids carrying the hsdS and hsdM genes of EcoAI
wild-type R.PspGI and its mutant expressed as pET21a-PspGI-WT and pET21a-PspGI-D138A in Escherichia coli strain ER2744
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