3.1.21.3: type I site-specific deoxyribonuclease

This is an abbreviated version!
For detailed information about type I site-specific deoxyribonuclease, go to the full flat file.

Reaction

endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolysed =

Synonyms

adenosine triphosphate-dependent deoxyribonuclease, ATP-dependent deoxyribonuclease, ATP-dependent DNase, BsaHI restriction-modification system, C.PvuII, CfrAI, deoxyribonuclease (ATP- and S-adenosyl-L-methionine dependent), deoxyribonuclease (ATP-dependent), DNase, EC 3.1.23, EC 3.1.24, EC 3.1.4.33, Eco377I, Eco394I, Eco585I, Eco646I, Eco777I, Eco826I, Eco851I, Eco912I, EcoA0ORF42P, EcoAI, EcoAO83I, EcoB, EcoBI, EcoDI, EcoDXXI, EcoEI, EcoGIV, EcoK, EcoKI, EcoKI type I DNA restriction enzyme, EcoKI type I restriction-modification system, EcoprrI, EcoR, EcoR124, EcoR124/3I, EcoR124I, EcoR124II, EcoRII modification enzyme, EcoRII RM gene complex, EcoRII system, endodeoxyribonuclease, Esp1396I, exodeoxyribonuclease, H91_orf206, H91_orf376, HpyAXII restriction-modification system, HsdM, HsdR, hsdS, KpnBI, More, MpnORFDAP, MpnORFDBP, nuclease, deoxyribo-, nuclease, deoxyribo-, ATP-dependent, PspGI endonuclease, PspGI restriction-modification system, R. BsaHI, R.EcoAI, R.EcoEI, R.EcoKI, R.EcoR124I restriction endonuclease, R.EcoR124II, R.EcoR124INT, R.HpyAXII, R.PspGI, REase, RecoK, restriction-modification system, Sau1, Sau1 type I restriction-modification system, Sau1 Type I RM system, SauMW2I, SauMW2II, SauN315I, SauN315II, StyLTIII, StySBI, StySEAI, StySGI, StySJI, StySKI, StySPI, StySQI, type I R-M enzyme, type I R-M system, type I restriction enzyme, type I restriction modification enzyme, type I restriction-modification enzyme, type I restriction-modification system, type I restriction-modification system EcoR124I, type IB restriction enzyme, type II R-M system, type II restriction-modification system

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters

                3.1.21.3 type I site-specific deoxyribonuclease

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Results	in table
7	Activating Compound
36239	AA Sequence
5	Application
1	CAS Registry Number
27	Cloned(Commentary)
19	Cofactor
10	Crystallization (Commentary)
5	General Information
7	Inhibitors
2	KM Value [mM]
3	Localization
8	Metals/Ions
22	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
71	Organism
27	PDB ID
2	pH Optimum
1	Posttranslational Modification
28	Protein Variants
23	Purification (Commentary)
3	Reaction
1	Reaction Type
66	Reference
68	Substrates and Products (Substrate)
16	Subunits
181	Synonyms
1	Temperature Optimum [°C]
1	Turnover Number [1/s]

Application

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Application on EC 3.1.21.3 - type I site-specific deoxyribonuclease

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

biotechnology

Escherichia coli

-

potential uses for EcoR124I as a nanoactuator within a biosensor in the field of bionanotechnology. It may be used as a molecular dynamo that can measure events from single molecules of DNA, providing a highly sensitive biosensor for detecting events that interrupt translocation events

700022

medicine

2 entries

additional information

2 entries

medicine

Escherichia coli

Q07736, Q47281

identification of a putative restriction-modification system EcoA0ORF42P in the commensal Escherichia coli strain A0 34/86 (O83: K24: H31), which is efficiently used at Czech paediatric clinics for prophylaxis and treatment of nosocomial infections and diarrhoea of preterm and newborn infants. This type I R-M system is a member of the type IB family, but is not an isoschizomer of either any prototype of the type IB members or any sequenced putative IB R-M systems. It is designated as EcoAO83I, the DNA recognition sequence of the EcoAO83I is GGA(8N)ATGC. Combination of the classical biochemical and bacterial genetics approaches with comparative genomics may contribute effectively to further classification of many other putative type-I enzymes, especially in clinical samples

696985

medicine

Escherichia coli A0 34/86 (O83:K24:H31)

-

identification of a putative restriction-modification system EcoA0ORF42P in the commensal Escherichia coli strain A0 34/86 (O83: K24: H31), which is efficiently used at Czech paediatric clinics for prophylaxis and treatment of nosocomial infections and diarrhoea of preterm and newborn infants. This type I R-M system is a member of the type IB family, but is not an isoschizomer of either any prototype of the type IB members or any sequenced putative IB R-M systems. It is designated as EcoAO83I, the DNA recognition sequence of the EcoAO83I is GGA(8N)ATGC. Combination of the classical biochemical and bacterial genetics approaches with comparative genomics may contribute effectively to further classification of many other putative type-I enzymes, especially in clinical samples

-

additional information

Staphylococcus aureus

-

inactivation of the hsdR gene of the Sau1 type I restriction-modification system is responsible for the high transformation efficiency of RN4220

695774

additional information

synthetic construct

-

transcription of both the restriction endonuclease and methyltransferase genes is controlled by interdependent regulatory loops governed by the C.Esp1396I protein

700507