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3.1.21.2: deoxyribonuclease IV

This is an abbreviated version!
For detailed information about deoxyribonuclease IV, go to the full flat file.

Word Map on EC 3.1.21.2

Reaction

Endonucleolytic cleavage of ssDNA at apurinic/apyrimidinic sites to 5'-phosphooligonucleotide end-products =

Synonyms

apurinic/apyrimidinic endonuclease, coliphage T4 endonuclease II, deoxriboendonuclease, DNA-adenine-transferase, E. coli endonuclease IV, EC 3.1.4.30, End, Endo IV, endodeoxyribonuclease IV, EndoII, EndoIV, endonuclease II, endonuclease IV, exonuclease III, More, MtbEndo IV, MtbNfo, MtbXthA, Nfo, nuclease, endodeoxyribo-oder redoxyendonuclease, Rv0670, Sco4631, ScoA3McrA, T4 endonuclease IV, TthNfo, type IV methyl-dependent restriction endonuclease

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters
                3.1.21.2 deoxyribonuclease IV

Purification

Purification on EC 3.1.21.2 - deoxyribonuclease IV

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
GST-Endo IV fusion protein
Tequatrovirus T4
-
GST-Endo IV fusion proteins: wild-type and mutants W88R and S176N
Tequatrovirus T4
-
phage T4 infected
-
Streptactin column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration
the gene is expressed under the direction of an IPTG-inducible T7 promoter, and the Thermotoga maritima endonuclease IV is purified as a His-tagged protein
using a amylose resin affinity column, the fusion protein is cleaved with thrombin to remove the MBP tag, the cleavage mixture is applied to a Q anion-exchange column