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3.1.21.1: deoxyribonuclease I

This is an abbreviated version!
For detailed information about deoxyribonuclease I, go to the full flat file.

Word Map on EC 3.1.21.1

Reaction

endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products =

Synonyms

alkaline deoxyribonuclease, alkaline DNase, alkaline nuclease, bovine pancreatic deoxyribonuclease I, bovine pancreatic DNase, bp-DNase I, bpDNase, carp liver DNase, ColE7, ColE9, colicin, colicin E2, Colicin E9, CRM197, deoxyribonuclease, deoxyribonuclease (pancreatic), deoxyribonuclease 1, deoxyribonuclease 1-like 3, deoxyribonuclease A, deoxyribonuclease I, deoxyribonuclease I-like enzyme, deoxyribonuclease-1, deoxyribonucleic phosphatase, desoxyribonuclease, diphtheria toxin, DNA endonuclease, DNA nuclease, DNA polymerase, DNAase, DNase, DNase 1, DNase A, DNase B, DNase C, DNase D, DNase I, DNase I-like enzyme, Dnase Y, DNase-I, Dnase1, DNase1-like 3, DNase1/3, DNASE1L3, DNaseI, domase, Dornase alfa, dornase alpha, dornava, dornavac, DTx, E9 Dnase, EC 3.1.4.5, endodeoxyribinuclease I, endodeoxyribonuclease I, EndoDNase, Escherichia coli endonuclease I, extracellular nuclease, hDNase I, lactoferrin, More, neutral DNase, neutral nuclease, nuclease Bh1, nuclease Stn beta, nuclease, deoxyribo-, nuclease, Escherichia coli endo-, I, NucT, OmpA, outer membrane protein A, pancreatic deoxyribonuclease, pancreatic DNase, pancreatic DNase I, pancreatic dornase, Potyviral Ia Proeinase, pulmozyme, rhDNaseI, PVBV proteinase, SdaD2, Slx1, Spd, Spd3, SsnA, streptodornase, tear lipocalin, thymonuclease, type I DNAse I, Vvn

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters
                3.1.21.1 deoxyribonuclease I

General Stability

General Stability on EC 3.1.21.1 - deoxyribonuclease I

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Ca2+ is able to protect DNase completely against inactivation by trypsin
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extremely sensitive to inactivation by proteases in the absence of Ca2+
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less active in phosphate buffer
N18 and N106 are both necessary for full enzymatic activity, heat-stability, and trypsin resistance
prolonged storage in the frozen state in H2O causes a gradual decrease of activity, half life of DNases A and B is 2 months
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sensitives to repeated freezing and thawing
stable at high concentrations of urea and organic solvents but susceptible to low concentrations of SDS and guanidine hydrochloride
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