3.1.13.B1: 5'-3' exoribonuclease
This is an abbreviated version!
For detailed information about 5'-3' exoribonuclease, go to the full flat file.
Word Map on EC 3.1.13.B1
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3.1.13.B1
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decapping
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sfrnas
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deadenylation
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flaviviruses
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exonucleolytic
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pyrophosphohydrolase
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exoribonucleolytic
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p-bodies
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pre-rrnas
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pacman
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flaviviral
- 3.1.13.B1
-
decapping
-
sfrnas
-
deadenylation
- flaviviruses
-
exonucleolytic
-
pyrophosphohydrolase
-
exoribonucleolytic
-
p-bodies
- pre-rrnas
-
pacman
-
flaviviral
Reaction
exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates =
Synonyms
5'-3' exoribonuclease 2, 5'-to-3' exoribonuclease, Pab-RNase J, PAB1751, Rat1, Saci-aCPSF2, Saci2362, Sso-RNase J, SSO0386, Tk-RNase J, Tk1469
ECTree
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General Information
General Information on EC 3.1.13.B1 - 5'-3' exoribonuclease
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evolution
yeast Rat1 is evolutionarily well conserved from yeast to human (Xrn2 in human)
physiological function
the enzyme plays a major role in mRNA turnover
physiological function
yeast Rat1 is an essential nuclear protein. Within a complex with Rai1, the 5'-3' exoribonuclease Rat1 promotes termination of RNA polymerase II (RNAPII) on protein-coding genes. The 5'-3' exoribonuclease activity of Rat1 is essential for RNAPII termination. Rat1 forms a complex with Rai1 that stabilizes Rat1 and helps target 5' monophosphate RNA by its diphosphohydrolase activity. Within a complex with Rai1, the 5'-3' exoribonuclease Rat1 promotes termination of RNAPII on protein-coding genes, overview. RNAPII is prone to more effective termination by Rat1/Rai1 when its catalytic site is disrupted due to NTP misincorporation, implying that paused RNAPII, which is often found in vivo near termination sites, can adopt a similar configuration to Rat1/Rai1 and trigger termination. Rat1/Rai1 itself is not sufficient to terminate RNAPII in vitro. Multiple-mechanistic features contribute to Rat1-mediated termination of RNAPII. NTP misincorporation induces RNAPII pausing and enhances termination by Rat1/Rai1, Rat1/Rai1 more effectively terminates RNAPII when a non-cognate NTP (ATP, CTP or UTP), rather than cognate GTP. Exoribonuclease activity is required for Rat1 not only to approach RNAPII but also to accumulate a sufficient driving force to dislodge the polymerase from the DNA template
physiological function
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the enzyme plays a major role in mRNA turnover
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