3.1.13.2: exoribonuclease H
This is an abbreviated version!
For detailed information about exoribonuclease H, go to the full flat file.
Word Map on EC 3.1.13.2
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3.1.13.2
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strand
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duplex
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nucleic
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rna-dna
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single-stranded
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integrase
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r-loops
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oligodeoxynucleotides
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retrotransposons
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heteroduplexes
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transcriptases
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phosphorothioate
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retroviruses
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polypurine
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moloney
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phosphodiester
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exonuclease
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rna-dependent
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pre-mrnas
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nucleases
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oligodeoxyribonucleotides
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plus-strand
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nnrti
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template-primer
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nucleocapsids
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minus-strand
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dntp
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spliceosome
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myeloblastosis
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thumb
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endonucleolytic
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oligonucleotide-directed
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2'-o-methylated
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nevirapine
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snrnp
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oligoribonucleotide
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primer-template
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okazaki
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nonnucleoside
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rna-directed
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a-form
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efavirenz
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retroelements
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3'-exonuclease
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heteropolymeric
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phosphoramidite
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pregenomic
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hepadnavirus
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hiv-rt
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pharmacology
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medicine
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drug development
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internucleotide
- 3.1.13.2
- strand
- duplex
- nucleic
- rna-dna
-
single-stranded
-
integrase
-
r-loops
- oligodeoxynucleotides
-
retrotransposons
- heteroduplexes
- transcriptases
- phosphorothioate
- retroviruses
-
polypurine
-
moloney
-
phosphodiester
-
exonuclease
-
rna-dependent
- pre-mrnas
- nucleases
- oligodeoxyribonucleotides
-
plus-strand
-
nnrti
-
template-primer
-
nucleocapsids
-
minus-strand
- dntp
-
spliceosome
-
myeloblastosis
-
thumb
-
endonucleolytic
-
oligonucleotide-directed
-
2'-o-methylated
- nevirapine
-
snrnp
- oligoribonucleotide
-
primer-template
-
okazaki
-
nonnucleoside
-
rna-directed
-
a-form
- efavirenz
-
retroelements
- 3'-exonuclease
-
heteropolymeric
-
phosphoramidite
-
pregenomic
-
hepadnavirus
- hiv-rt
- pharmacology
- medicine
- drug development
-
internucleotide
Reaction
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid =
Synonyms
3'-to-5' RNase H, HIV RNase H, HIV-1 ribonuclease H, HIV-1 RT ribonuclease H, LC11-RNase H1, More, Prp8, retroviral reverse transcriptase RNaseH, retroviral RNase H, reverse transcriptase ribonuclease H, reverse transcriptase-associated ribonuclease H, ribonuclease H, RNase H, RNase H1, RNase HI, RNaseH, RNH, RNH1, RT RNase H, RT/RNase H, T4 RNase H, Ta11
ECTree
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Turnover Number
Turnover Number on EC 3.1.13.2 - exoribonuclease H
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0.0135
DNA-15-nucleotide-RNA hybrid
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pH 7.8, 37°C, recombinant wild-type enzyme
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0.017
DNA-16-nucleotide-RNA hybrid
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pH 7.8, 37°C, recombinant wild-type enzyme
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0.0018
DNA-17-nucleotide-RNA hybrid
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pH 7.8, 37°C, recombinant wild-type enzyme
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0.0032
DNA-18-nucleotide-RNA hybrid
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pH 7.8, 37°C, recombinant wild-type enzyme
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additional information
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study of the order of cleavage, most RNA fragments are cleaved by a combination of primary, secondary primary, and 5-nt cuts within 1 min
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additional information
additional information
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kcat value of Avian myeloblastosis virus reverse transcriptase (RNase H reaction) is above 0.025 s-1. The kcat values for different Moloney murine leukaemia virus/Avian myeloblastosis virus chimeric reverse transcriptases with poly(rA)*p(dT)45 as a substrate: 6.3 1/s for MRT-AF, 3.8 1/s for MRT-AP, 4.6 1/s for MRT-AT, and 2.4 1/s for MRT-AR
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additional information
additional information
kcat value of Moloney murine leukemia virus reverse transcriptase (RNase H reaction) above 3 ms-1. The kcat values for different Moloney murine leukemia virus/avian myeloblastosis virus chimeric reverse transcriptases with poly(rA)*p(dT)45 as a substrate: 6.3 1/s for MRT-AF, 3.8 1/s for MRT-AP, 4.6 1/s for MRT-AT, and 2.4 1/s for MRT-AR
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additional information
additional information
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kcat value of Moloney murine leukemia virus reverse transcriptase (RNase H reaction) above 3 ms-1. The kcat values for different Moloney murine leukemia virus/avian myeloblastosis virus chimeric reverse transcriptases with poly(rA)*p(dT)45 as a substrate: 6.3 1/s for MRT-AF, 3.8 1/s for MRT-AP, 4.6 1/s for MRT-AT, and 2.4 1/s for MRT-AR
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