3.1.13.2: exoribonuclease H
This is an abbreviated version!
For detailed information about exoribonuclease H, go to the full flat file.
Word Map on EC 3.1.13.2
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3.1.13.2
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strand
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duplex
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nucleic
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rna-dna
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single-stranded
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integrase
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r-loops
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oligodeoxynucleotides
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retrotransposons
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heteroduplexes
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transcriptases
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phosphorothioate
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retroviruses
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polypurine
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moloney
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phosphodiester
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exonuclease
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rna-dependent
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pre-mrnas
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nucleases
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oligodeoxyribonucleotides
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plus-strand
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nnrti
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template-primer
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nucleocapsids
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minus-strand
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dntp
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spliceosome
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myeloblastosis
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thumb
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endonucleolytic
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oligonucleotide-directed
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2'-o-methylated
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nevirapine
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snrnp
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oligoribonucleotide
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primer-template
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okazaki
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nonnucleoside
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rna-directed
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a-form
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efavirenz
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retroelements
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3'-exonuclease
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heteropolymeric
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phosphoramidite
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pregenomic
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hepadnavirus
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hiv-rt
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pharmacology
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medicine
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drug development
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internucleotide
- 3.1.13.2
- strand
- duplex
- nucleic
- rna-dna
-
single-stranded
-
integrase
-
r-loops
- oligodeoxynucleotides
-
retrotransposons
- heteroduplexes
- transcriptases
- phosphorothioate
- retroviruses
-
polypurine
-
moloney
-
phosphodiester
-
exonuclease
-
rna-dependent
- pre-mrnas
- nucleases
- oligodeoxyribonucleotides
-
plus-strand
-
nnrti
-
template-primer
-
nucleocapsids
-
minus-strand
- dntp
-
spliceosome
-
myeloblastosis
-
thumb
-
endonucleolytic
-
oligonucleotide-directed
-
2'-o-methylated
- nevirapine
-
snrnp
- oligoribonucleotide
-
primer-template
-
okazaki
-
nonnucleoside
-
rna-directed
-
a-form
- efavirenz
-
retroelements
- 3'-exonuclease
-
heteropolymeric
-
phosphoramidite
-
pregenomic
-
hepadnavirus
- hiv-rt
- pharmacology
- medicine
- drug development
-
internucleotide
Reaction
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid =
Synonyms
3'-to-5' RNase H, HIV RNase H, HIV-1 ribonuclease H, HIV-1 RT ribonuclease H, LC11-RNase H1, More, Prp8, retroviral reverse transcriptase RNaseH, retroviral RNase H, reverse transcriptase ribonuclease H, reverse transcriptase-associated ribonuclease H, ribonuclease H, RNase H, RNase H1, RNase HI, RNaseH, RNH, RNH1, RT RNase H, RT/RNase H, T4 RNase H, Ta11
ECTree
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IC50 Value
IC50 Value on EC 3.1.13.2 - exoribonuclease H
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0.0005
(E)-3,4-dihydroxy-N'-((2-methoxynaphthalen-1-yl)methylene)benzohydrazide
Human immunodeficiency virus 1
-
0.0374
2,7-dihydroxyisoquinoline-1,3(2H,4H)-dione
Human immunodeficiency virus 1
-
pH 8.0, 37°C
0.0353
2-(4-fluorophenyl)-N-(2-hydroxy-1,3-dioxo-1,2,3,4-tetrahydroisoquinolin-7-yl)acetamide
Human immunodeficiency virus 1
-
pH 8.0, 37°C
0.00043
2-hydroxy-4H-isoquinoline-1,3-dione
Human Immunodeficiency Virus
-
specific inhibition of isolated RNase H domain and of the full length reverse transcriptase, IC50 value for the isolated RNase H domain is 0.00043 mM
0.0072
2-hydroxy-7-(4-hydroxyphenyl)isoquinoline-1,3(2H,4H)-dione
Human immunodeficiency virus 1
-
pH 8.0, 37°C
0.058
2-hydroxy-7-iodoisoquinoline-1,3(2H,4H)-dione
Human immunodeficiency virus 1
-
pH 8.0, 37°C
0.048
2-hydroxy-7-nitroisoquinoline-1,3(2H,4H)-dione
Human immunodeficiency virus 1
-
pH 8.0, 37°C
0.0107
2-hydroxy-7-[4-(trifluoromethyl)phenyl]isoquinoline-1,3(2H,4H)-dione
Human immunodeficiency virus 1
-
pH 8.0, 37°C
0.0585
2-[(10Z)-heptadec-10-en-1-yl]-6-hydroxybenzoic acid
Human immunodeficiency virus 1
-
-
0.0005
3,4-dihydroxy-N'-[(E)-(2-methoxynaphthalen-1-yl)methylidene]benzohydrazide
Human immunodeficiency virus 1
-
IC50 value is about 0.5 microM
0.0075
4'-chloro-N'-[(1E)-(2-methoxynaphthalen-1-yl)methylidene]biphenyl-4-carbohydrazide
Human immunodeficiency virus 1
-
0.00002 - 0.001
4-(1-chloro-1,1-difluoromethyl)-4-(2-phenylethynyl)-6-chloro-2H-3,1-benzoxazin-2-one
0.002
4-(dimethylamino)-N'-[(1E)-(2-methoxynaphthalen-1-yl)methylidene]benzohydrazide
Human immunodeficiency virus 1
-
0.012
4-methoxy-N'-[(1E)-(2-methoxynaphthalen-1-yl)methylidene]benzohydrazide
Human immunodeficiency virus 1
-
0.003
4-tert-butyl-N'-[(1E)-(2-methoxynaphthalen-1-yl)methylidene]benzohydrazide
Human immunodeficiency virus 1
-
0.002 - 0.0035
4-[5-(benzoylamino)thien-2-yl]-2,4-dioxobutanoic acid
Human immunodeficiency virus 1
-
a diketo acid RNase H inhibitor, IC50 values for wild-type and mutant enzymes are 0.002-0.0035 mM with all substrates, effect on mutant enzymes, overview
0.00038
5-chloro-2-hydroxyisoquinoline-1,3(2H,4H)-dione
Human immunodeficiency virus 1
-
inhibits the isolated HIV RNase H domain
0.0026 - 0.0322
5-nitrofuran-2-carboxylic acid [[4-(4-bromophenyl)-thiazol-2-yl]-(tetrahydro-furan-2-yl-methyl)-carbamoyl] methyl ester
0.0086
5-nitrofuran-2-carboxylic acid [[4-(4-bromophenyl)-thiazol-2-yl]-(tetrahydrofuran-2-ylmethyl)-carbamoyl] methyl ester
murine leukemia virus
-
for murine leukemia virus reverse transcriptase
0.0066
7-(3,4-dihydroxyphenyl)-2-hydroxyisoquinoline-1,3(2H,4H)-dione
Human immunodeficiency virus 1
-
pH 8.0, 37°C
0.0222
7-(4-fluorophenyl)-2-hydroxyisoquinoline-1,3(2H,4H)-dione
Human immunodeficiency virus 1
-
pH 8.0, 37°C
0.0289
7-chloro-2-hydroxyisoquinoline-1,3(2H,4H)-dione
Human immunodeficiency virus 1
-
pH 8.0, 37°C
0.013
ethyl (5E)-6-[1-(4-fluorobenzyl)-1H-pyrrol-2-yl]-2,4-dioxohex-5-enoate
Human immunodeficiency virus 1
-
-
0.015
N'-[(1E)-(2-methoxynaphthalen-1-yl)methylidene]-4-methylbenzohydrazide
Human immunodeficiency virus 1
-
0.007
N'-[(1E)-(2-methoxynaphthalen-1-yl)methylidene]-4-phenoxybenzohydrazide
Human immunodeficiency virus 1
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0.015
N'-[(1E)-(2-methoxynaphthalen-1-yl)methylidene]benzohydrazide
Human immunodeficiency virus 1
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0.005
N'-[(1E)-(2-methoxynaphthalen-1-yl)methylidene]biphenyl-4-carbohydrazide
Human immunodeficiency virus 1
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0.0108
N-(2-hydroxy-1,3-dioxo-1,2,3,4-tetrahydroisoquinolin-7-yl)-3-nitrobenzamide
Human immunodeficiency virus 1
-
pH 8.0, 37°C
0.0071
N-(2-hydroxy-1,3-dioxo-1,2,3,4-tetrahydroisoquinolin-7-yl)-4-fluorobenzamide
Human immunodeficiency virus 1
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pH 8.0, 37°C
0.0407
N-(2-hydroxy-1,3-dioxo-1,2,3,4-tetrahydroisoquinolin-7-yl)acetamide
Human immunodeficiency virus 1
-
pH 8.0, 37°C
0.0057
N-(2-hydroxy-1,3-dioxo-1,2,3,4-tetrahydroisoquinolin-7-yl)benzamide
Human immunodeficiency virus 1
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pH 8.0, 37°C
0.003
N-(4-tert-butylbenzoyl)-2-hydroxy-1-naphthaldehyde hydrazone
Human immunodeficiency virus 1
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IC50 is about 3 microM
0.025
Phosphonoformate
Human immunodeficiency virus 1
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the inhibitor that bind in the reverse transcriptase polymerase domain inhibits also RNase H activity, IC value for the wild-type enzyme is 0.0015 mM versus substrate DNA-18-nucleotide-RNA hybrid, with the other substrates the IC50 value is above 0.025 mM
0.00024
1,3,4,5-tetragalloylapiitol
Human immunodeficiency virus 1
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also inhibitory effective against HIV-2 RNase H with an IC50 value of 0.13 microM. Poor selectivity against human RNase H with an IC50 value of 1.5 microM
0.002
Human immunodeficiency virus 1
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pH 8.0, 37°C
0.0025
2-amino-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxamide
Human immunodeficiency virus 2
-
pH 8.0, 37°C
0.0066
2-amino-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxamide
Human immunodeficiency virus 1
-
wild-type, pH 8.0, 37°C
0.02
2-amino-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxamide
Human immunodeficiency virus 1
-
Y501F mutant, pH 8.0, 37°C
0.0706
2-amino-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxamide
Human immunodeficiency virus 1
-
Y501W mutant, pH 8.0, 37°C
0.0896
2-amino-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxamide
Human immunodeficiency virus 1
-
Y501Az-F mutant, pH 8.0, 37°C
0.1967
2-amino-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxamide
Human immunodeficiency virus 1
-
Y501Bp-F mutant, pH 8.0, 37°C
0.00043
Human immunodeficiency virus 1
-
inhibits the isolated HIV RNase H domain in the assay using CGK1 as substrate
0.0006
2-hydroxyisoquinoline-1,3(2H,4H)-dione
Human immunodeficiency virus 1
-
using CGK1 as substrate
0.001
2-hydroxyisoquinoline-1,3(2H,4H)-dione
Human immunodeficiency virus 1
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in another assay, using poly(dC:rG) as a template, without inhibitory effect against Escherichia coli RNase H
0.00002 - 0.00005
Human immunodeficiency virus 1
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i.e. NNRTI, an efavirenz analogue, IC50 values for wild-type enzyme are 0.00002-0.00005 mM with substrates DNA-17-nucleotide-RNA hybrid or DNA-18-nucleotide-RNA hybrid
0.001
4-(1-chloro-1,1-difluoromethyl)-4-(2-phenylethynyl)-6-chloro-2H-3,1-benzoxazin-2-one
Human immunodeficiency virus 1
-
0.001 mM for substrate DNA-15-nucleotide-RNA hybrid or DNA-16-nucleotide-RNA hyb
0.0032
Human immunodeficiency virus 1
-
HIV-1 RNase H inhibitor, which inhibits the HIV-1 RNase H as part of HIV-reverse transcriptase without influencing the activity of HIV-1-dependent RNA and DNA polymerase
0.0047
4-[5-(benzoylamino)tien-2-yl]-2,4-dioxobutanoic acid
Human immunodeficiency virus 1
-
isolated HIV-RNAse H domain
0.0038
Human immunodeficiency virus 1
for the CRF01 A_E (93JPNH1) reverse transcriptase. pH 8.0, 37°C
0.0084
5-nitrofuran-2-carboxylic acid adamantan-1-carbamoyl methyl ester
murine leukemia virus
-
for murine leukemia virus reverse transcriptase, pH 7.8, 37°C
0.0265
5-nitrofuran-2-carboxylic acid adamantan-1-carbamoyl methyl ester
Human immunodeficiency virus 1
for the HIV-1 clade C (93IN101) reverse transcriptase. pH 8.0, 37°C
0.0296
5-nitrofuran-2-carboxylic acid adamantan-1-carbamoyl methyl ester
Human immunodeficiency virus 1
for clade B HIV-1LAI-derived reverse transcriptase-associated RNase H activity. pH 8.0, 37°C
0.0026
5-nitrofuran-2-carboxylic acid [[4-(4-bromophenyl)-thiazol-2-yl]-(tetrahydro-furan-2-yl-methyl)-carbamoyl] methyl ester
Human immunodeficiency virus 1
for the CRF01 A_E (93JPNH1) reverse transcriptase. pH 8.0, 37°C
0.0267
5-nitrofuran-2-carboxylic acid [[4-(4-bromophenyl)-thiazol-2-yl]-(tetrahydro-furan-2-yl-methyl)-carbamoyl] methyl ester
Human immunodeficiency virus 1
for clade B HIV-1LAI-derived reverse transcriptase-associated RNase H activity. pH 8.0, 37°C
0.0322
5-nitrofuran-2-carboxylic acid [[4-(4-bromophenyl)-thiazol-2-yl]-(tetrahydro-furan-2-yl-methyl)-carbamoyl] methyl ester
Human immunodeficiency virus 1
for the HIV-1 clade C (93IN101) reverse transcriptase. pH 8.0, 37°C
0.0015
ardimerin digallate
Human immunodeficiency virus 1
-
without inhibiting both human and Escherichia coli RNase H at a concentration of 200 microM/l
0.00065
dihydroxy benzoyl naphthyl hydrazone
Human immunodeficiency virus 1
-
drug-resistant HIV-1 reverse transcriptase variant Y181C reverse transcriptase
0.0012
dihydroxy benzoyl naphthyl hydrazone
Human immunodeficiency virus 1
-
drug-resistant HIV-1 reverse transcriptase variant Y188l reverse transcriptase
0.0032
Human immunodeficiency virus 1
-
pH 8.0, 37°C
0.005
N-[3-(aminocarbonyl)-4,5-dimethyl-2-thienyl]-2-furancarboxamide
Human immunodeficiency virus 2
-
pH 8.0, 37°C
0.01
nevirapine
Human immunodeficiency virus 1
-
above, pH 8.0, 22°C, mutants K103N, Y181C, Y188L, and K103N/Y181C
additional information
additional information
Human immunodeficiency virus 1
inhibition of mutant enzymes by DHBNH and antiviral activity, overview
-
additional information
additional information
Human immunodeficiency virus 1
-
inhibition of mutant enzymes by DHBNH and antiviral activity, overview
-
additional information
additional information
Human immunodeficiency virus 1
-
IC50 more than 18 microM for hyemaloside B
-
additional information
additional information
Human immunodeficiency virus 1
-
IC50 value is higher than 96.00 microM for 7-bromo-2-hydroxyisoquinoline-1,3(2H,4H)-dione. IC50 value is higher than 80.0 microM for N-(2-hydroxy-1,3-dioxo-1,2,3,4-tetrahydroisoquinolin-7-yl)pentanamide, for 2-hydroxy-7-phenylisoquinoline-1,3(2H,4H)-dione, and for N-(2-hydroxy-1,3-dioxo-1,2,3,4-tetrahydroisoquinolin-7-yl)-2-phenylacetamide. pH 8.0, 37°C
-
additional information
additional information
Human immunodeficiency virus 1
-
IC50 values of different oligonucleotides with structural characters of hairpins and dumbbells: 0.000500 mM for oligonucleotide with 35 base pairs based on the G-quartet motif, 0.0258 mM for 1R4RR4, 0.0262 mM for 2R4RR4, 0.0693 mM for 3R4RR4, 0.0078 mM for 6R6RR6, 0.0297 mM for 7R6RR6, 0.0404 mM for 8nicked dumbbell, and 0.0033 mM for 9ligared dumbbell. IC50 value is much higher than 100 microM for 4D4RD4 and 5R4D4R. [(4-chlorophenyl)hydrazono] propanedioic acid shows an IC50 value of 3 microM against polymerase-independent RNase H activity. Its activity against HIV-1 RNase H in vivo and toxicity in cell culture or in vivo still need to be investigated. IC50 0.0013 mM for 3,7-dihydroxy-tropolone, monosubstituted, and 0.0047 mM for 3,7-dihydroxy-tropolone, unsubstituted
-
additional information
additional information
Human immunodeficiency virus 1
-
the IC50 values for 3-[(8Z)-pentadec-8-en-1-yl]phenol, 3-[(10Z)-heptadec-10-en-1-yl]phenol, 3-tridecylphenol, 3-pentadecylphenol, 5-[(8Z)-pentadec-8-en-1-yl]benzene-1,3-diol, and 5-tridecylbenzene-1,3-diol are above 200 microM
-
additional information
additional information
Human immunodeficiency virus 1
-
Y5014-(phenylcarbonyl)-L-Phe mutant, the IC50 value is above 300 microM
-