3.1.13.2: exoribonuclease H
This is an abbreviated version!
For detailed information about exoribonuclease H, go to the full flat file.
Word Map on EC 3.1.13.2
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3.1.13.2
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strand
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duplex
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nucleic
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rna-dna
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single-stranded
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integrase
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r-loops
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oligodeoxynucleotides
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retrotransposons
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heteroduplexes
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transcriptases
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phosphorothioate
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retroviruses
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polypurine
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moloney
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phosphodiester
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exonuclease
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rna-dependent
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pre-mrnas
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nucleases
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oligodeoxyribonucleotides
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plus-strand
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nnrti
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template-primer
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nucleocapsids
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minus-strand
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dntp
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spliceosome
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myeloblastosis
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thumb
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endonucleolytic
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oligonucleotide-directed
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2'-o-methylated
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nevirapine
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snrnp
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oligoribonucleotide
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primer-template
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okazaki
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nonnucleoside
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rna-directed
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a-form
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efavirenz
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retroelements
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3'-exonuclease
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heteropolymeric
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phosphoramidite
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pregenomic
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hepadnavirus
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hiv-rt
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pharmacology
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medicine
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drug development
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internucleotide
- 3.1.13.2
- strand
- duplex
- nucleic
- rna-dna
-
single-stranded
-
integrase
-
r-loops
- oligodeoxynucleotides
-
retrotransposons
- heteroduplexes
- transcriptases
- phosphorothioate
- retroviruses
-
polypurine
-
moloney
-
phosphodiester
-
exonuclease
-
rna-dependent
- pre-mrnas
- nucleases
- oligodeoxyribonucleotides
-
plus-strand
-
nnrti
-
template-primer
-
nucleocapsids
-
minus-strand
- dntp
-
spliceosome
-
myeloblastosis
-
thumb
-
endonucleolytic
-
oligonucleotide-directed
-
2'-o-methylated
- nevirapine
-
snrnp
- oligoribonucleotide
-
primer-template
-
okazaki
-
nonnucleoside
-
rna-directed
-
a-form
- efavirenz
-
retroelements
- 3'-exonuclease
-
heteropolymeric
-
phosphoramidite
-
pregenomic
-
hepadnavirus
- hiv-rt
- pharmacology
- medicine
- drug development
-
internucleotide
Reaction
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid =
Synonyms
3'-to-5' RNase H, HIV RNase H, HIV-1 ribonuclease H, HIV-1 RT ribonuclease H, LC11-RNase H1, More, Prp8, retroviral reverse transcriptase RNaseH, retroviral RNase H, reverse transcriptase ribonuclease H, reverse transcriptase-associated ribonuclease H, ribonuclease H, RNase H, RNase H1, RNase HI, RNaseH, RNH, RNH1, RT RNase H, RT/RNase H, T4 RNase H, Ta11
ECTree
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Crystallization
Crystallization on EC 3.1.13.2 - exoribonuclease H
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in complex with selenium-modified DNA/RNA, sitting drop vapor diffusion method, using 0.1 M MES pH 6.5, 12% (w/v) PEG 20000
Halalkalibacterium halodurans
crystal structures of full-length HIV-1 reverse transcriptase with a DNA/DNA or RNA/DNA substrate show a distance of about 60 A between the polymerase active site and the RNase H active site
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enzyme bound to inhibitor (E)-3,4-dihydroxy-N'-((2-methoxynaphthalen-1-yl)methylene)benzohydrazide, by vapor diffusion in microseeded hanging drops containing equal volumes of protein and reservoir solution containing 50 mM imidazole, pH 6.4, 100 mM ammonium sulfate, 15 mM magnesium sulfate, 5% glucose, 11.5% PEG 8000 at 4°C, mixing 0.0074 ml of 20 mM inhibitor in DMSO with 0.0025 ml of 20% beta-octyl glucopyranoside, of this solution, 0.0075 ml is combined with 0.065 ml of 40 mg/ml RT and 0.0575 ml of additional RT buffer containing 10 mM Tris, pH 8.0, and 75 mM NaCl on ice, X-ray diffraction structure determination and anaylsis at 3.15 A resolution
more: solution of structure of enzyme is determined by NMR methods in presence of Mg2+
purified recombinant mutant Mo-MLV RNase H lacking the putative helix C complexed with the DNA/RNA hybrid substrate, DELTAC monomers and dimers, equilibration against a reservoir solution of 30% PEG 4000 and 0.2 M ammonium sulfate, protein solution, containing 7-10 mg/ml, 10 mM HEPES, pH 7.0, 150 mM NaCl, 5 mM DTT, and 0.1 mM EDTA, is mixed with reservoir solution at pH 5.2-6.0, DELTAC monomer crystallization fails to yield single, large crystals, DELTAC monomer crystals are soaked in 15% PEG 4000, 20% PEG 400, 0.1 M ammonium sulfate, 150 mM NaCl, 1% 2-propanol, 1.25% PEG MME 550, 5 mM MES, pH 6.5, and 0.5 mM zinc sulfate for 5 min, X-ray diffractions tructure determination and analysis at 1.6 A resolution, modelling
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sitting drop vapor diffusion method, using 0.1 M imidazole pH 8.0, 0.2 M NaCl, 0.4 M NaH2PO4, and 1.6 M K2HPO4
hanging drop vapor diffusion method, using 40% (w/v) PEG1500, 100 mM Na citrate pH 4.7, and 5 mM MgCl2
sitting drop vapor diffusion method, using 27% (w/v) polyethylene glycol 1500 and 0.1 M sodium citrate, pH 4.7