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3.1.13.2: exoribonuclease H

This is an abbreviated version!
For detailed information about exoribonuclease H, go to the full flat file.

Word Map on EC 3.1.13.2

Reaction

3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid =

Synonyms

3'-to-5' RNase H, HIV RNase H, HIV-1 ribonuclease H, HIV-1 RT ribonuclease H, LC11-RNase H1, More, Prp8, retroviral reverse transcriptase RNaseH, retroviral RNase H, reverse transcriptase ribonuclease H, reverse transcriptase-associated ribonuclease H, ribonuclease H, RNase H, RNase H1, RNase HI, RNaseH, RNH, RNH1, RT RNase H, RT/RNase H, T4 RNase H, Ta11

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.13 Exoribonucleases producing 5′-phosphomonoesters
                3.1.13.2 exoribonuclease H

Cloned

Cloned on EC 3.1.13.2 - exoribonuclease H

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli BL21(DE3) Codon Plus RIL cells
expressed in Escherichia coli BL21(DE3)[pLysS] cells
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expressed in Escherichia coli BL21-CodonPlus(DE3) cells
expressed in Escherichia coli MIC2067(DE3) cells
expressed in Escherichia coli Rosetta DE3 cells
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expressed in Escherichia coli Rosetta2(DE3)pLysS cells
expressed in Escherichia coli with 6-histidine tags
expression in Escherichia coli
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expression in Escherichia coli and expression in bacteria by plasmids
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expression of His-tagged wild-type and mutant reverse transcriptases in Escherichia coli
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expression of HIV-1 reverse transcriptase/RNase H in Escherichia coli strain BL21(DE3)
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expression of RNase H /reverse transcriptase from HIV-1 and HIV-2 in Escherichia coli
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expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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expression of wild-type and mutant enzymes, containing a Factor Xa cleavage site and a GST-tag, in Escherichia coli strain BL21(DE3)
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expression of wild-type reverse transcriptase in fusion with the viral inactivated protease mutant D24A in Escherichia coli, the recombinant fusion protein shows reverse transcriptase activity, i.e. DNA polymerase activity with an RNA template, and RNase activity, and also strand displacement activity, overview
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gene HXB2, expression as maltose-binding-protein fusion protein containing a TEV cleavae site in Escherichia coli strain BL21(DE3), functional expression of His-tagged isolated RNase H domain in Escherichia coli strain BL21(DE3)
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gene rnh, DNA and amino acid sequence determination and analysis of the rnh genes from phages T4D, das13, NG163das13 and NG163, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain L21(DE3)/pLysE
Tequatrovirus T4
HBV RNaseH sequences from genotype C isolate V01460 encoding residues corresponding to amino acids 688 to 844 of the HBV polymerase (reference strain ADW2, genotype A) are cloned by gene synthesis into pMAL-c5X-His with sequences encoding a His6-tag appended to the 3' end of the RNaseH gene to create pMal-HRHgtC. Sequences encoding Ala-Gly-Ala are inserted between the maltose binding protein (MBP) and HBV RNaseH sequences, and sequences encoding Gly-Ala-Gly are inserted between sequences for the RNaseH and the His6-tag. Recombinant expression of wild-type and mutant His6-tagged enzymes in Escherichia coli strain BL21(DE3)
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recombinant expression of His-tagged HBV ribonuclease H1 in Escherichia coli
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RNase H domain
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