3.1.12.1: 5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming)
This is an abbreviated version!
For detailed information about 5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming), go to the full flat file.
Reaction
exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates =
Synonyms
5' to 3' single stranded DNA exonuclease, BH0340, Cas4, Cas4 nuclease, Cas4-1, Cas4-2, casC, CRISPR adaptation factor, CRISPR-associated Cas4 nuclease, CRISPR-associated exonuclease Cas4, CRISPR-associated protein 4, DUF83Pc, DUF83Ss, Pcal_0546, PF1119, PF1793, SSO0001, TTV1 nucleocapsid protein TP1, type I-C Cas4
ECTree
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Purification
Purification on EC 3.1.12.1 - 5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming)
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recombinant His-tagged enzyme Cas4 from Escherichia coli by nickel affinity chromatography, co-elution of a Cas4-Cas1-Cas2 complex from nickel affinity purification
Halalkalibacterium halodurans