3.1.11.5: exodeoxyribonuclease V

This is an abbreviated version!
For detailed information about exodeoxyribonuclease V, go to the full flat file.

Reaction

Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides =

Synonyms

AddAB enzyme, E. coli ATP-dependent DNase, E. coli exonuclease V, Escherichia coli exonuclease V, Escherichia coli RecBCD, Exodeoxyribonuclease V 125 kDa polypeptide, Exodeoxyribonuclease V 135 KDA polypeptide, Exodeoxyribonuclease V 67 kDa polypeptide, exonuclease V, ExoV, gene recBC DNase, gene RecBC endoenzyme, nuclease, exodeoxyribo V, PAB2263, RecB, RecB helicase-nuclease, REcB30 protein, recBC deoxyribonuclease, recBC DNase, recBC nuclease, RecBCD, RecBCD DNase, recBCD enzyme, RecBCD exonuclease, RecBCD-type helicase-nuclease, RecD, UPF0286 protein PYRAB01260

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.11 Exodeoxyribonucleases producing 5′-phosphomonoesters

                3.1.11.5 exodeoxyribonuclease V

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Results	in table
18	Activating Compound
30379	AA Sequence
11	Application
1	CAS Registry Number
14	Cloned(Commentary)
7	Cofactor
3	Crystallization (Commentary)
14	General Information
3	General Stability
36	Inhibitors
12	KM Value [mM]
1	Localization
51	Metals/Ions
50	Molecular Weight [Da]
107	Natural Substrates/ Products (Substrates)
116	Organism
51	PDB ID
20	pH Optimum
3	pH Range
25	Protein Variants
23	Purification (Commentary)
7	Reaction
2	Reaction Type
85	Reference
3	Specific Activity [micromol/min/mg]
7	Storage Stability
246	Substrates and Products (Substrate)
20	Subunits
41	Synonyms
9	Temperature Optimum [°C]
5	Temperature Range [°C]
2	Temperature Stability [°C]
5	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 3.1.11.5 - exodeoxyribonuclease V

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, purified enzyme RecBC, 1 year, 80% loss of activity

Escherichia coli

-

134026

-20°C, purified enzyme RecBCD, 1 year, 10%-20% loss of activity

Escherichia coli

-

134026

-20°C, purified enzyme, 1 month, 35% loss of activity

Escherichia coli

-

134015

-20°C, purified enzyme, 7 months, 80% loss of activity

Escherichia coli

-

134015

-70°C, purified enzyme, 6 weeks, 0% loss of activity

Escherichia coli

-

134015

0°C, Tris, MgCl2, EDTA, mercaptoethanol, 1 d, 5-10% loss of activity

Escherichia coli

-

133998

4°C, RecC, stable for several months

Escherichia coli

-

651426