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3.1.11.5: exodeoxyribonuclease V

This is an abbreviated version!
For detailed information about exodeoxyribonuclease V, go to the full flat file.

Word Map on EC 3.1.11.5

Reaction

Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides =

Synonyms

AddAB enzyme, E. coli ATP-dependent DNase, E. coli exonuclease V, Escherichia coli exonuclease V, Escherichia coli RecBCD, Exodeoxyribonuclease V 125 kDa polypeptide, Exodeoxyribonuclease V 135 KDA polypeptide, Exodeoxyribonuclease V 67 kDa polypeptide, exonuclease V, ExoV, gene recBC DNase, gene RecBC endoenzyme, nuclease, exodeoxyribo V, PAB2263, RecB, RecB helicase-nuclease, REcB30 protein, recBC deoxyribonuclease, recBC DNase, recBC nuclease, RecBCD, RecBCD DNase, recBCD enzyme, RecBCD exonuclease, RecBCD-type helicase-nuclease, RecD, UPF0286 protein PYRAB01260

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.11 Exodeoxyribonucleases producing 5′-phosphomonoesters
                3.1.11.5 exodeoxyribonuclease V

Molecular Weight

Molecular Weight on EC 3.1.11.5 - exodeoxyribonuclease V

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
107000
-
1 * 115000 + 1 * 107000 + 1 * 68000
115000
-
1 * 115000 + 1 * 107000 + 1 * 68000
128000
-
1 * 140000 + 1 * 128000, dissociation by boiling, SDS-PAGE
130000
-
1 * 140000 + 1 * 130000 + 1 * 65000, SDS-PAGE
134000
-
recB protein, amino acid analysis
135000
-
1 * 135000 + 1 * 141000, addA and addB gene encode two subunits of enzyme, the enzyme carries two homologous nuclease domains
140000
141000
-
1 * 135000 + 1 * 141000, addA and addB gene encode two subunits of enzyme, the enzyme carries two homologous nuclease domains
155000
-
1 * 155000 + 1 * 140000, protease inhibitor phenylmethylsulfonyl flouride was added
170000
-
1 * 170000 + 1 * 60000, glycerol gradient sedimentation
220000
-
sedimentation, calculated
270000
308000
333000
-
gel filtration, SDS-PAGE
350000
42500
-
1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor
52500
-
1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor
58000
-
recD protein, gel filtration, SDS-PAGE
60000
62000
-
1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor
65000
-
1 * 140000 + 1 * 130000 + 1 * 65000, SDS-PAGE
655000
-
recB protein, gel filtration, glycerol gradient centrifugation
66970
-
alpha subunit, recD protein, amino acid analysis
68000
-
1 * 115000 + 1 * 107000 + 1 * 68000
70000
-
1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor
81000
-
1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor
99000
-
sedimentation, calculated