3.1.11.2: exodeoxyribonuclease III
This is an abbreviated version!
For detailed information about exodeoxyribonuclease III, go to the full flat file.
Word Map on EC 3.1.11.2
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3.1.11.2
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strand
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single-stranded
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polymerases
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duplex
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dicer
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endonucleases
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dsrnas
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bacteriophage
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proofreading
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endoribonuclease
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mismatch
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drosha
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ultrasensitive
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klenow
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nick
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stem-loop
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ssdna
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abasic
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exonucleolytic
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aptamer
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nucleases
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polynucleotide
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translesion
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dntp
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aptasensors
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argonaut
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ape1
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pri-mirnas
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misincorporation
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g-quadruplexes
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overhang
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pnpase
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exonuclease-deficient
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microprocessor
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dsrna-binding
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primase
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tetraloops
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iii-dependent
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micrococcal
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aphidicolin
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pre-rrnas
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primer-template
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dnazyme
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rna-induced
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foscarnet
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template-primer
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synthesis
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target-induced
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medicine
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rna-specific
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analysis
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molecular biology
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okazaki
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dicer-like
- 3.1.11.2
- strand
-
single-stranded
- polymerases
- duplex
- dicer
- endonucleases
- dsrnas
- bacteriophage
-
proofreading
-
endoribonuclease
- mismatch
- drosha
-
ultrasensitive
-
klenow
- nick
-
stem-loop
- ssdna
-
abasic
-
exonucleolytic
- aptamer
- nucleases
- polynucleotide
-
translesion
- dntp
-
aptasensors
-
argonaut
- ape1
-
pri-mirnas
-
misincorporation
-
g-quadruplexes
-
overhang
- pnpase
-
exonuclease-deficient
-
microprocessor
-
dsrna-binding
- primase
- tetraloops
-
iii-dependent
-
micrococcal
- aphidicolin
- pre-rrnas
-
primer-template
- dnazyme
-
rna-induced
- foscarnet
-
template-primer
- synthesis
-
target-induced
- medicine
-
rna-specific
- analysis
- molecular biology
-
okazaki
-
dicer-like
Reaction
Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates =
Synonyms
3'-5' exonuclease, 3'-5' exonuclease III, AP endonuclease VI, AP-endonuclease/3'-5'exodeoxyribonuclease, Ape2, class II apurinic/apyrimidinic-endonuclease/3'-5' exonuclease III, class-II AP-endonuclease, DNA pol, E. coli exonuclease III, EC 3.1.4.27, Escherichia coli exonuclease III, exo III, EXO-3, exodeoxyribonuclease III, ExoIII, exonuclease III, exonuclease-3, MacExoIII, More, MtbXthA, nuclease, endoribo-, III, nuclease, Escherichia coli exo-, III, ribonuclease III, RNase III, Sso, xthA, xthA-1 gene product
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Reaction
Reaction on EC 3.1.11.2 - exodeoxyribonuclease III
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Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates
four activities of enzyme: 3-5 exonuclease, RNase H, 3-phosphatase and AP endonuclease
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Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates
XthA exonuclease converts the blunt ends of double-stranded DNA to 5' protruding ends in the process of in vivo cloning. After the insert and the vector DNA fragments are introduced into the Escherichia coli cell, XthA resects the ends of the DNA fragments from the 3'-to-5' direction, producing 5' overhanging ends. As the ends of insert and vector DNAs have mutually complementary sequences, the 5' overhanging ends of the insert and the vector DNA fragments hybridize to each other as cohesive ends. In addition, the gaps are filled by DNA polymerases and the nicks are repaired by DNA ligases