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3.1.11.2: exodeoxyribonuclease III

This is an abbreviated version!
For detailed information about exodeoxyribonuclease III, go to the full flat file.

Word Map on EC 3.1.11.2

Reaction

Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates =

Synonyms

3'-5' exonuclease, 3'-5' exonuclease III, AP endonuclease VI, AP-endonuclease/3'-5'exodeoxyribonuclease, Ape2, class II apurinic/apyrimidinic-endonuclease/3'-5' exonuclease III, class-II AP-endonuclease, DNA pol, E. coli exonuclease III, EC 3.1.4.27, Escherichia coli exonuclease III, exo III, EXO-3, exodeoxyribonuclease III, ExoIII, exonuclease III, exonuclease-3, MacExoIII, More, MtbXthA, nuclease, endoribo-, III, nuclease, Escherichia coli exo-, III, ribonuclease III, RNase III, Sso, xthA, xthA-1 gene product

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.11 Exodeoxyribonucleases producing 5′-phosphomonoesters
                3.1.11.2 exodeoxyribonuclease III

Cloned

Cloned on EC 3.1.11.2 - exodeoxyribonuclease III

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression as a GST-fusion protein in Saccharomyces cerevisiae
-
expression in Escherichia coli
into the Gateway entry vector pENTR3C, for expressing Ape2 proteins in the yeast strain BJ5464, the cDNAs are cloned in fusion with GST using the plasmid pBJ842
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recombinant expression of wild-type and mutant enzymes
the enzyme containing the mitochondrial targeting signal of manganese superoxide dismutase is expressed in a human malignant breast epithelial cell line, MDA-MB-231, this expression results in a decrease in cell viability following exposure to oxidative stress
-