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3.1.11.2: exodeoxyribonuclease III

This is an abbreviated version!
For detailed information about exodeoxyribonuclease III, go to the full flat file.

Word Map on EC 3.1.11.2

Reaction

Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates =

Synonyms

3'-5' exonuclease, 3'-5' exonuclease III, AP endonuclease VI, AP-endonuclease/3'-5'exodeoxyribonuclease, Ape2, class II apurinic/apyrimidinic-endonuclease/3'-5' exonuclease III, class-II AP-endonuclease, DNA pol, E. coli exonuclease III, EC 3.1.4.27, Escherichia coli exonuclease III, exo III, EXO-3, exodeoxyribonuclease III, ExoIII, exonuclease III, exonuclease-3, MacExoIII, More, MtbXthA, nuclease, endoribo-, III, nuclease, Escherichia coli exo-, III, ribonuclease III, RNase III, Sso, xthA, xthA-1 gene product

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.11 Exodeoxyribonucleases producing 5′-phosphomonoesters
                3.1.11.2 exodeoxyribonuclease III

Application

Application on EC 3.1.11.2 - exodeoxyribonuclease III

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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
medicine
molecular biology
in contrast to DNA cloning utilizing in vitro recombination, some strains of Escherichia coli can take up linear double-stranded vectors, insert DNA fragments, and assemble them in vivo involving exonulcease III. The ends of these linear DNA fragments must contain 20 to 50 bp of overlapping homologous sequences. Improved protocols for in vivo cloning have realized a high level of usability comparable to that by in vitro recombination reactions, but using the exonuclease III, it is only necessary to introduce PCR products into Escherichia coli for the in vivo cloning
synthesis
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enzyme is used for purification of eucaryotic extrachromosomal circular DNAs using exonuclease III
additional information
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using the cleavage function of exonuclease III on double-stranded DNA, design of an amplified DNA detection scheme employing a stem-loop DNA molecular beacon as the signaling probe. Exonuclease III used to recycle target molecules, thus leading to improved sensitivity relative to traditional molecular beacons without any significant restriction in the choice of target sequences