2.1.1.B76: flavone/flavonol 7-O-methyltransferase
This is an abbreviated version!
For detailed information about flavone/flavonol 7-O-methyltransferase, go to the full flat file.
Reaction
Synonyms
flavone 7-O-methyltransferase, flavonoid 7-O-methyltransferase, flavonoid O-methyltransferase, POMT-7, POMT7
ECTree
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Engineering
Engineering on EC 2.1.1.B76 - flavone/flavonol 7-O-methyltransferase
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D257G
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random mutagenesis, the mutant of POMT-7, POMT7-M1, has a novel regioselectivity, from large scale mutant screening, mutant D257G methylates the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group
N257G
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the mutant methylates the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group. The wild type POMT7 exhibits better activity toward kaempferol and quercetin than the mutant enzyme
additional information
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optimization of POMT7 expression and rhamnetin production in Escherichia coli, in addition, the S-adenosylmethionine biosynthesis pathway is engineered by coexpression of SAM synthetase, for optimal cofactor production, production of rhamnetin of up to 111 mg/l, overview
additional information
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combines engineered and optimization of enzyme expression and activity with cofacor production in recombinant Escherichia coli for improved biosynthesis of rhamnetin