2.1.1.B43: tRNA (cytosine49-C5)-methyltransferase

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For detailed information about tRNA (cytosine49-C5)-methyltransferase, go to the full flat file.

Reaction

Synonyms

PAB1946

ECTree

2 Transferases

2.1 Transferring one-carbon groups

2.1.1 Methyltransferases

2.1.1.B43 tRNA (cytosine49-C5)-methyltransferase

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Results	in table
1	Activating Compound
1	AA Sequence
1	Cloned(Commentary)
2	Molecular Weight [Da]
1	Organism
1	Purification (Commentary)
1	Reaction
1	Reference
1	Substrates and Products (Substrate)
1	Subunits
1	Synonyms
1	Systematic Name

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Substrates Products on EC 2.1.1.B43 - tRNA (cytosine49-C5)-methyltransferase

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S-adenosyl-L-methionine + cytosine49 in tRNA

S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA

Pyrococcus abyssi

Q9V106

tRNAASp. In vitro, the purified recombinant methyltransferase catalyzes 5-methylcytosine formation at several cytosines within tRNAs with preference for C49. When tRNA is incubated with the PAB1947 enzyme at 50°C instead of 80°C, methylation becomes more specific for the 4751 sequence. Addition of archease to the methylation reactions with Pyrococcus abyssi tRNAAsp at 80°C increases modification within the oligonucleotide ACCCG that contains cytidine49, while reducing modification at unspecific sites. The unstructured poly(C)RNA is effectively methylated at 50°C, with most of the tritiated label from the methyl donor becoming incorporated into the RNA. However, when the recombinant PAB1947 enzyme is substituted with a Pyrococcus abyssi cell extract, no methylation of the poly(C) RNA occurs

708990