2.1.1.95: tocopherol C-methyltransferase
This is an abbreviated version!
For detailed information about tocopherol C-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.95
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2.1.1.95
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alpha-tocopherol
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lipophilic
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delta-t
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beta-tocopherols
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brassica
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homogentisate
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nitrotyrosine
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alpha-t
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phytyltransferase
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lipid-soluble
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chlorophyl
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biotechnology
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nutrition
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synthesis
- 2.1.1.95
- alpha-tocopherol
-
lipophilic
-
delta-t
- beta-tocopherols
-
brassica
- homogentisate
-
nitrotyrosine
-
alpha-t
-
phytyltransferase
-
lipid-soluble
-
chlorophyl
- biotechnology
- nutrition
- synthesis
Reaction
Synonyms
gamma tocopherol methyl transferase, gamma-TLMT, gamma-TMT, gamma-TMT1, gamma-TMT2, gamma-TMT3, gamma-tocopherol methyltransferase, gamma-tocopherol-like N-methyltransferase, gamma-tocopherol/tocotrienol methyltransferase, gammaTMT, PiNMT, S-adenosyl-L-methionine:gamma-tocopherol 5-O-methyltransferase, TMT, tocopherol O-methyltransferase, VTE4
ECTree
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Engineering
Engineering on EC 2.1.1.95 - tocopherol C-methyltransferase
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additional information
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a Solanum tuberosum cDNA fragment gamma-TMT is introduced into intron-spliced hairpin RNA, RNAi, silencing construct driven by the constitutive cauliflower mosaic virus 35S promoter. RNAi knockdown of gamma-TMT results in an up to 95% reduction of alpha-tocopherol in leaves of transgenic plants, which is almost quantitatively compensated for by an increase in gamma-tocopherol. gamma-TMT-silenced plants show an improved performance when challenged with sorbitol or methyl viologen, but salt tolerance of gamma-TMT transgenic plants is strongly decreased, compared to the wild-type plants, phenotype, overview
additional information
overexpression of the enzyme leads to increased alpha-tocopherol content in transgenic Glycine max plant seeds, 10.4fold increased alpha-tocopherol content and 14.9fold increased beta-tocopherol content in T2 seeds, tocopherol contents in the seeds from T0 and T1 transgenic soybean plants, overview
additional information
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overexpression of the enzyme leads to increased alpha-tocopherol content in transgenic Glycine max plant seeds, 10.4fold increased alpha-tocopherol content and 14.9fold increased beta-tocopherol content in T2 seeds, tocopherol contents in the seeds from T0 and T1 transgenic soybean plants, overview