2.1.1.90: methanol-corrinoid protein Co-methyltransferase

This is an abbreviated version!
For detailed information about methanol-corrinoid protein Co-methyltransferase, go to the full flat file.

Reaction

Synonyms

Cob(I)alamin methyltransferase, methanol cobalamin methyltransferase, methanol-5-hydroxybenzimidazolylcobamide Co-methyltransferase, methanol-coenzyme M-methyltransferase complex, methanol:5-hydroxy-benzimidazolylcobamide methyltransferase, methanol:5-hydroxybenzimidazolylcobamide methyltransferase, methanol:coenzyme M methyltransferase, methanol:CoM methyltransferase complex, methyltransferase, methanol-cobalamin, MMPA:cob(I)alamin methyltransferase, MT 1, MT1, MtaABC, MtaB, MtpA

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.90 methanol-corrinoid protein Co-methyltransferase

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Results	in table
4	Activating Compound
21	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
4	Cofactor
1	Crystallization (Commentary)
1	Expression
2	General Information
1	General Stability
6	Inhibitors
1	kcat/KM [mM/s]
2	KM Value [mM]
13	Metals/Ions
7	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
26	Organism
2	Oxidation Stability
3	Pathway
16	PDB ID
2	pH Optimum
1	pI Value
6	Purification (Commentary)
1	Reaction
1	Reaction Type
22	Reference
1	Specific Activity [micromol/min/mg]
22	Substrates and Products (Substrate)
4	Subunits
32	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 2.1.1.90 - methanol-corrinoid protein Co-methyltransferase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

mercaptopropionate + methylcob(III)alamin

?

Methanosarcina acetivorans

-

-

757103

-

-

?

methanol + 5-hydroxybenzimidazolylcobamide

Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O

4 entries

methanol + a [Co(I) methanol-specific corrinoid protein]

a [methyl-Co(III) methanol-specific corrinoid protein] + H2O

9 entries

methanol + cob(I)alamin

methyl-cob(III)alamin + H2O

4 entries

methanol + cob(I)alamin

methylcob(III)alamin + H2O

Methanosarcina acetivorans

-

methylation of free cob(I)alamin by methylmercaptopropionate, which is the physiologically relevant direction, is observed only in the presence of very high levels of methylmercaptopropionate

757103

-

-

?

additional information

?

-

2 entries

methanol + 5-hydroxybenzimidazolylcobamide

Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O

Methanosarcina acetivorans

-

methanol catabolism in Methanosarcina species requires the concerted effort of methanol:5-hydroxybenzimidazolylcobamide methyltransferase (MtaB), a corrinoid-containing methyl-accepting protein (MtaC) and Co-methyl-5-hydroxybenzimidazolylcobamide:2-mercapto-ethanesulfonic acid methyltransferase (MtaA). Methanosarcina acetivorans possesses three operons encoding putative methanol-specific MtaB and corrinoid proteins: mtaCB1, mtaCB2 and mtaCB3. Deletion mutants lacking the three operons, in all possible combinations, are constructed and characterized. Strains deleted for any two of the operons grow on methanol, whereas strains lacking all three do not

681980

-

-

?

methanol + 5-hydroxybenzimidazolylcobamide

Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O

Methanosarcina barkeri

Q46EH3

-

485660

-

485660

?

methanol + 5-hydroxybenzimidazolylcobamide

Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O

Methanosarcina barkeri

Q46EH3

-

485666

-

485666

?

methanol + 5-hydroxybenzimidazolylcobamide

Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O

Methanosarcina barkeri

Q46EH3

one of the enzymes responsible for the transmethylation from methanol to coenzyme M

485660

-

485660

?

methanol + a [Co(I) methanol-specific corrinoid protein]

a [methyl-Co(III) methanol-specific corrinoid protein] + H2O

Methanomethylovorans hollandica

-

-

756784

-

-

?

methanol + a [Co(I) methanol-specific corrinoid protein]

a [methyl-Co(III) methanol-specific corrinoid protein] + H2O

Methanosarcina barkeri

-

-

727819, 756784

-

-

?

methanol + a [Co(I) methanol-specific corrinoid protein]

a [methyl-Co(III) methanol-specific corrinoid protein] + H2O

Methanosarcina barkeri

-

first of two enzymes involved in the transmethylation reaction from methanol to 2-mercaptoethanesulfonic acid in. The enzyme only binds the methyl group of methanol when the cobalt atom of its corrinoid prosthetic groups is present in the highly reduced Co(I) state. Formation of this redox state requires H2, hydrogenase, methyltransferase activation protein, and ATP. Purified enzyme contains 1.7 corrinoids per enzyme with cobalt in the fully oxidized Co(III) state. Water and N-3 of the 5-hydroxybenzimidazolyl base serve as the upper and lower ligands, respectively. Reduction to the Co(II) level is accomplished by H2 and hydrogenase. The cob(II)amide of the enzyme has the base coordinated at this stage. Subsequent addition of methyltransferase activation protein and ATP results in the formation of base-uncoordinated Co(II) enzyme

727819

-

-

?

methanol + a [Co(I) methanol-specific corrinoid protein]

a [methyl-Co(III) methanol-specific corrinoid protein] + H2O

Methanosarcina barkeri DSM 800

-

-

727819

-

-

?

methanol + a [Co(I) methanol-specific corrinoid protein]

a [methyl-Co(III) methanol-specific corrinoid protein] + H2O

Methanosarcina barkeri DSM 800

-

first of two enzymes involved in the transmethylation reaction from methanol to 2-mercaptoethanesulfonic acid in. The enzyme only binds the methyl group of methanol when the cobalt atom of its corrinoid prosthetic groups is present in the highly reduced Co(I) state. Formation of this redox state requires H2, hydrogenase, methyltransferase activation protein, and ATP. Purified enzyme contains 1.7 corrinoids per enzyme with cobalt in the fully oxidized Co(III) state. Water and N-3 of the 5-hydroxybenzimidazolyl base serve as the upper and lower ligands, respectively. Reduction to the Co(II) level is accomplished by H2 and hydrogenase. The cob(II)amide of the enzyme has the base coordinated at this stage. Subsequent addition of methyltransferase activation protein and ATP results in the formation of base-uncoordinated Co(II) enzyme

727819

-

-

?

methanol + a [Co(I) methanol-specific corrinoid protein]

a [methyl-Co(III) methanol-specific corrinoid protein] + H2O

Methanosarcina mazei

-

-

756784

-

-

?

methanol + a [Co(I) methanol-specific corrinoid protein]

a [methyl-Co(III) methanol-specific corrinoid protein] + H2O

Methanosarcina mazei

Q8PXZ6

-

726582

-

-

?

methanol + a [Co(I) methanol-specific corrinoid protein]

a [methyl-Co(III) methanol-specific corrinoid protein] + H2O

Methanosarcina mazei DSM 3647

-

-

726582

-

-

?

methanol + a [Co(I) methanol-specific corrinoid protein]

a [methyl-Co(III) methanol-specific corrinoid protein] + H2O

Methanosphaera stadtmanae

-

-

756784

-

-

?

methanol + cob(I)alamin

methyl-cob(III)alamin + H2O

Methanosarcina barkeri

Q46EH3

-

485666

-

485666

?

methanol + cob(I)alamin

methyl-cob(III)alamin + H2O

Methanosarcina barkeri

Q48949; Q46EH3; Q46EH4

-

756274

-

-

r

methanol + cob(I)alamin

methyl-cob(III)alamin + H2O

Methanosarcina barkeri

Q46EH3

catalyzed by beta subunit MtaB alone

485665

-

485665

r

methanol + cob(I)alamin

methyl-cob(III)alamin + H2O

Methanosarcina barkeri DSM 804

Q48949; Q46EH3; Q46EH4

-

756274

-

-

r

additional information

?

-

Methanosarcina acetivorans

-

analysis of mtaCB gene regulation in Methanosarcina acetivorans

680470

-

-

?

additional information

?

-

Methanosarcina acetivorans

-

coenzyme M does not serve as a methyl acceptor. Methylation of cob(I)alamin with methanol, dimethylsulfide, dimethylamine, and methyl-CoM is not observed, even at high substrate concentrations

757103

-

-

-