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2.1.1.57: methyltransferase cap1

This is an abbreviated version!
For detailed information about methyltransferase cap1, go to the full flat file.

Word Map on EC 2.1.1.57

Reaction

S-adenosyl-L-methionine
+
a 5'-(N7-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA]
=
S-adenosyl-L-homocysteine
 +
a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]

Synonyms

(nucleoside-2'-O-)-methyltransferase, (nucleoside-2'O)-methyltransferase, 2'-O methyltransferase, 2'-O MTase, 2'-O-methyltransferase, 2'-O-MTase, 2'O methyltransferase, 2'O-MTase, 2'OMTases, cap-specific 2'-O-methyltransferase, cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1, cap1 2-O'-ribose methyltransferase, CMTr1, EC 2.1.1.58, FtsJ methyltransferase domain-containing 2, FTSJD2, ISG95, KIAA0082, L protein domain VI, large polymerase protein domain VI, messenger ribonucleate nucleoside 2'-methyltransferase, messenger RNA (nucleoside-2'-)-methyltransferase, methyltransferase, messenger ribonucleate nucleoside 2'-, mRNA (nucleoside-2'-O)-methyltransferase, mRNA 5' cap-specific (nucleoside-2'-O)-methyltransferase, mRNA cap (nucleoside-2'-O)-methyltransferase, mRNA cap methyltransferase, mRNA cap-specific 2'-O-methyltransferase, MT57, Mtr1, nonstructural protein 16, nonstructural protein 16 methyltransferase, nonstructural protein 5 methyltransferase, NS5, NS5 MTase, NS5MTase, nsp10/16 complex, nsp10/nsp16 complex, nsp16, nsp16 protein, nsp16/nsp10 protein complex, ribose 2'-O methyltransferase, RNA (nucleoside-2'O)-methyltransferase, RNA cap 2'-O-methyltransferase, RNA cap 2'-O-MTase, RNA cap 2'O-MTase, RNA cap1 methyltransferase, RNA capping enzyme domain in protein A, S-adenosyl-L-methionine-dependent mRNA cap MTase, TbMT48, TbMT57, VP39, VP4

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.57 methyltransferase cap1

Purification

Purification on EC 2.1.1.57 - methyltransferase cap1

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography on ADP-Sepharose
-
by centrifugation
-
by gel filtration
fragment mutant and exchange mutants from recombinant E. coli
-
GSTPrep 16/10 column chromatography
-
HeLa-cells, phosphocellulose chromatography
-
HisPur cobalt resin column chromatography
-
HiTrap heparin column chromatography and HIS Select nickel affinity gel filtration and HiTrap Q column chromatography
-
mutants recombinant from E. coli
-
Ni-NTA resin column chromatography
-
Ni-NTA resin column chromatography and Superdex 200 gel filtration
nickel-nitrilotriacetic acid resin column chromatography and heparin-Sepharose 6 column chromatography
-
recombinant from Spodoptera frugiperda cells
-
recombinant His-tagged N-terminal part of NS5 protein from E. coli
Sepharose resin column chromatography and Superdex 200 gel filtration
-
solubilized from viral core
-