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2.1.1.56: mRNA (guanine-N7)-methyltransferase

This is an abbreviated version!
For detailed information about mRNA (guanine-N7)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.56

Reaction

S-adenosyl-L-methionine
+
a 5'-(5'-triphosphoguanosine)-[mRNA]
=
S-adenosyl-L-homocysteine
 +
a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]

Synonyms

(guanine-N7)-methyltransferase, Abd1, alphavirus capping enzyme, cap methyltransferase, cap N7-MTase, cap N7MTase, cap-methyltransferase, DENV methyltransferase, DENV-4 NS5, Ecm1, guanine-7-methyltransferase, guanine-N-7 methyltransferase, guanine-N7 methyltransferase, guanosine-7-methyltransferase, messenger ribonucleate guanine 7-methyltransferase, messenger RNA guanine 7-methyltransferase, methyltransferase domain of Sudan ebolavirus L protein, methyltransferase, messenger ribonucleate guanine 7-, mRNA cap (guanine N-7) methyltransferase, mRNA cap (guanine N-7) MTase, mRNA cap (guanine-N7) methyltransferase, mRNA cap methyltransferase, mRNA guanosine cap by RNA guanine-7-methyltransferase, MTase+CTD, N-7-MTase, N7 methyltransferase, N7 MTase, N7-methyltransferase, N7-MTase, non-structural protein 1, nonstructural protein 14, nonstructural protein 5 methyltransferase, NS5, NS5MTase, nsp 14, nsP1 methyltransferase, nsP1 MTase, nsp14, RNA (guanine-N7) methyltransferase, RNA cap (guanine N-7) methyltransferase, RNA guanine-7 methyltransferase, RNA guanine-7-methyltransferase, RNA guanine-methyltransferase, RNA guanine-N7 methyltransferase, RNA N7-MTase, RNMT, TbCgm1, TbCmt1

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.56 mRNA (guanine-N7)-methyltransferase

Cloned

Cloned on EC 2.1.1.56 - mRNA (guanine-N7)-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 carboxyl-truncated versions of the large subunit of the mRNA capping enzyme, expression in Escherichia coli
-
as a His6-Smt3 fusion protein in Escherichia coli BL21(DE3) CodonPlus RIL using a pET-based pSmt3-TOPO vector
-
coexpression of genes D1 and D12 from 1 plasmid
-
complementation of knock-down HeLa S3 cells with enzymatically active N-truncated enzyme comprising residues 120-476 and being located at the nucleus, no complementation of knock-down HeLa S3 cells with enzymatically active N-truncated enzyme comprising residues 144-476 and being loacetd mainly in the cytoplasm, truncated enzymes are expresss in fusion with GFP
-
D1 fragment, amino acids 498 to 844
-
D1 fragment, amino acids 520-844
-
expressed in Escherichia coli Rosetta (DE3) cells
expressed in Escherichia coli T7 cells
-
expressed in HEK-293 cells and Escherichia coli
-
expressed in Saccharomyces cerevisiae strains YBS40, YBS2, and YBS20
-
expression in Escherichia coli
-
expression in Escherichia coli, His-tagged
expression in Escherichia coli, His-tagged, amino acid sequence analysis
expression in Saccharomyces cerevisiae, His-tagged, amino acid sequence analysis
expression of 2 different variants, each with and without His-tag, in Escherichia coli, amino acid sequence analysis
-
expression of D1 fragment mutants in Escherichia coli
-
expression of full length and 2 truncated genes in Saccharomyces cerevisiae and Escherichia coli, location of methyltransferase activity
-
expression of full length enzyme and truncated enzyme in yeast and in HeLa cells, His-tagged, as GFP-fusion protein, or cMyc-tagged
-
expression of N-terminally His6-tagged enzyme, residues 1-298, as Smt3-fusion protein in Escherichia coli strain BL21(DE3) or strain B834DE3, expression of wild-type and mutant alleles under control of a yeast promotor in a Saccharomyces cerevisiae abd1DELTA null mutant strain
-
expression of VP4 protein in Spodoptera frugiperda cells
-
His6-tagged proteins are expressed in Escherichia coli BL21(DE3) cells and in Saccharomyces cerevisiae strain YBS40
-
in vitro transcription and translation in presence of liposomes, enzyme is attached to the liposomes surface
-
individual and coexpression of His-tagged D1 large subunit fragment, being the methyltransferase domain of the enzyme, and of D12 small subunit in Escherichia coli
-
inserted into vector pET16b and transformed into Escherichia coli BL21(DE3), yeast strain YBS40 transformed with CEN TRP1 plasmids containing the wild-type and mutant alleles of ECM1
into vector pDest14 and transformed into Escherichia coli Rosetta(DE3) cells
-
TbCmt1 inserted into an inducible T7 RNA polymerase-based pET vector and introduced into Escherichia coli BL21(DE3)Gold
-
the MTase domain, representing the N-terminal 272 amino acids of DENV-4 NS5 is cloned and purified
-
vaccinia virus genes D1 and D12 encoding the large and the small subunit of the mRNA capping enzyme, respectively, individual overexpression in Escherichia coli BL21
-
wild-type or mutant vD12, into the pRS413T vector, expressed in Saccharomyces cerevisiae strain YBS40
-
yeast strain YBS40 transformed with CEN TRP1 plasmids containing the wild-type and mutant alleles of ECM1, the pET16-Ecm1 plasmids introduced into Escherichia coli BL21(DE3)