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2.1.1.45: thymidylate synthase

This is an abbreviated version!
For detailed information about thymidylate synthase, go to the full flat file.

Word Map on EC 2.1.1.45

Reaction

5,10-methylenetetrahydrofolate
+
dUMP
=
dihydrofolate
 +
dTMP

Synonyms

BgDHFR-TS, bifunctional TS-DHFR, DFR-TS, DHFR-TS, DHFR–TS, dihydrofolate reductase-thymidylate synthase, dTMP synthase, HTS, human TS, LBRM_06_0830, methylenetetrahydrofolate:dUMP C-methyltransferase, More, Thy1, ThyA, thymidylate synthase, thymidylate synthase A, thymidylate synthase-dihydrofolate reductase, thymidylate synthetase, ThyX, TMP synthetase, TMPS, TS, TS-DHFR, TSase, TYMS, Y110A7A.4

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.45 thymidylate synthase

Storage Stability

Storage Stability on EC 2.1.1.45 - thymidylate synthase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 40% glycerol, stable over 2 months, 50% loss of activity by 24 h storage without glycerol
-20°C, activity remains unchanged after 1 month
-
-20°C, after 5 months less than 25% activity is lost
-
-20°C, can be stored in 10% glycerol, 0.05 M Tris-HCl, pH7.4 and 10 mM 2-mercaptoethanol for 30 days with a loss of approximately 50% activity
-
-20°C, enzyme can be stored frozen for several months without significant loss of activity
-
-20°C, in presence of 0.1% Triton X-100 and 10% glycerol, no loss of activity when stored in lysis buffer for several weeks
-
-20°C, occasionally loses activity
-
-20°C, pH 6.0, stable over a period of 1 month
-20°C, purified recombinant protein, stable at pH 6.0 over a period of 1 month
-20°C, sulfate-precipitated enzyme in 0.1 M potassium phosphate, 0.01 M dithiothreitol, pH 7.1can be kept at least 4 months with only 25% loss of activity
-
-20°C, TS activity of crude enzyme remains unchanged for 1 month of storage, purified enzyme loses 50% of its activity within 3-4 weeks
-
-25°C enzyme retains activity for 3 months when stored in presence of thiols, without thiols enzyme loses all activity in 2 days
-
-50°C-to -20°C, activity of purified enzyme remains unchanged for 2-3 months
-
-70°C preparation with 0.1% bovine serum albumin is stable up to 8 weeks of storage
-
-80°C, 40% glycerol, stable over 2 months
-80°C, stable upon storage in Tris-HCl pH 7.4 and thiol
-80°C, storage in 30% glycerol stabilizes enzyme
-
0-10°C enzyme is stable in buffer D for several weeks
-
0-5°C, enzyme is stable over a period of months in presence of 10 mM 2-mercaptoethanol in capped vessels layered with argon
-
0-7°C, concentrated enzyme keeps its activity for 14 days
-
0°C, enzyme can be maintained in its active form in presence of saccharose or glycerol for several days
-
0°C, enzyme can be stored in a concentrated solution for 2 weeks without a loss in activity
-
0°C, enzyme rapidly loses its activity, more than 90% in 2 h
-
4°C enzyme can be maintained for at least 4 months with little or no loss in activity
-
4°C enzyme retains activity for 3 months when stored in presence of thiols, without thiols enzyme loses all activity in 2 days
-
4°C, enzyme loses half of its activity upon storage for a few days
-
4°C, enzyme retains activity for about 3 weeks when stored with 20 mM dithiothreitol and 0.1 mM dUMP, without dithiothreitol purified enzyme is inactivated totally in 2 days, without dUMP enzyme keeps 50% activity after 1 week
-
4°C, in presence of 0.1% Triton X-100 and 10% glycerol, no loss of activity when stored in lysis buffer for at least 2 weeks
-
5°C half-life in buffered solution only 2 days
-