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2.1.1.45: thymidylate synthase

This is an abbreviated version!
For detailed information about thymidylate synthase, go to the full flat file.

Word Map on EC 2.1.1.45

Reaction

5,10-methylenetetrahydrofolate
+
dUMP
=
dihydrofolate
 +
dTMP

Synonyms

BgDHFR-TS, bifunctional TS-DHFR, DFR-TS, DHFR-TS, DHFR–TS, dihydrofolate reductase-thymidylate synthase, dTMP synthase, HTS, human TS, LBRM_06_0830, methylenetetrahydrofolate:dUMP C-methyltransferase, More, Thy1, ThyA, thymidylate synthase, thymidylate synthase A, thymidylate synthase-dihydrofolate reductase, thymidylate synthetase, ThyX, TMP synthetase, TMPS, TS, TS-DHFR, TSase, TYMS, Y110A7A.4

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.45 thymidylate synthase

KM Value

KM Value on EC 2.1.1.45 - thymidylate synthase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.07
(-)-5,10-methylene-tetrahydropteroylglutamate
-
-
0.061
(6S)-tetrahydropteroylglutamate
-
-
0.025
(l)-tetrahydropteroyltriglutamate
-
-
0.3
(R)-5,10-methylene-5,6,7,8-tetrahydrofolate
30fold higher than wild-type thymidylate synthase
0.3
(R)-5,10-methylenetetrahydrofolate
mutant K48Q
0.15
2'-deoxycytidine 5'-monophosphate
-
mutant TS N229D
0.0015
2'-deoxyuridine 5'-monophosphate
-
-
0.0007 - 0.0981
2'-deoxyuridylate
0.0137 - 0.0155
2'-deoxyuridylate 5'-dithiophosphate
0.00632 - 0.037
2'-deoxyuridylate 5'-thiophosphate
0.11 - 0.113
2'-fluoro-2'-deoxyuridine 5'-phosphate
0.00068
5,10-formyltetrahydropteroylheptaglutamate
-
-
0.00074
5,10-methenyltetrahydropteroylpentaglutamate
-
-
0.014
5,10-methylene-5,6,7,8-tetrahydrofolate
-
-
0.0015 - 0.3125
5,10-methylenetetrahydrofolate
0.031
5,10-methylenetetrahydropteroylglutamate
-
-
0.0022
5,10-methylenetetrahydropteroylpentaglutamate
-
-
0.0097 - 0.514
5-bromo-2'-deoxyuridine
0.0057 - 0.046
5-bromo-2'-deoxyuridylate
0.00085 - 0.179
dUMP
0.0045
L-tetrahydrofolate
-
-
0.008 - 0.13
methylenetetrahydrofolate
0.001 - 0.0118
methylenetetrahydropteroylgluamate
0.0211
N5,N10-methylenetetrahydrofolate
-
-
0.077
tetrahydropteroylglutamate
-
-
0.0065
[6R]methylenetetrahydropteroyldiglutamate
-
-
0.0043
[6R]methylenetetrahydropteroylglutamate
-
-
0.0043
[6R]methylenetetrahydropteroylheptaglutamate
-
-
0.0087
[6R]methylenetetrahydropteroylhexaglutamate
-
-
0.0083
[6R]methylenetetrahydropteroylpentaglutamate
-
-
0.0109
[6R]methylenetetrahydropteroyltetraglutamate
-
-
0.0035
[6R]methylenetetrahydropteroyltriglutamate
-
-
additional information
additional information
-