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2.1.1.45: thymidylate synthase

This is an abbreviated version!
For detailed information about thymidylate synthase, go to the full flat file.

Word Map on EC 2.1.1.45

Reaction

5,10-methylenetetrahydrofolate
+
dUMP
=
dihydrofolate
 +
dTMP

Synonyms

BgDHFR-TS, bifunctional TS-DHFR, DFR-TS, DHFR-TS, DHFR–TS, dihydrofolate reductase-thymidylate synthase, dTMP synthase, HTS, human TS, LBRM_06_0830, methylenetetrahydrofolate:dUMP C-methyltransferase, More, Thy1, ThyA, thymidylate synthase, thymidylate synthase A, thymidylate synthase-dihydrofolate reductase, thymidylate synthetase, ThyX, TMP synthetase, TMPS, TS, TS-DHFR, TSase, TYMS, Y110A7A.4

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.45 thymidylate synthase

General Stability

General Stability on EC 2.1.1.45 - thymidylate synthase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, storage in 30% glycerol stabilizes enzyme
-
comparatively unstable, more stable in 20% sucrose solution
-
enzyme can be kept active for about 2-3 weeks in buffer F in presence of 0.001 mM methotrexate, dialysis against dilute buffer leads to rapid irreversible inactivation as does freeze-drying or simple freezing
-
enzyme is not inactivated by freezing and thawing
-
enzyme is significantly stabilized by dUMP
-
enzyme is stabilized by addition of 20-120 mM dithiothreitol + bovine serum albumin
-
freezing and freeze-drying or dialysis causes rapid inactivation
-
glycerol and Triton X-100 stabilize the enzyme
quite unstable
stable to protein denaturants and limited proteolysis
-
stable upon quick-freezing in liquid nitrogen in buffer containing 150 mM KCl
thawing of frozen enzyme leads to irreversible precipitation of the protein and total loss of activity, no successfully lyophilization possible
-
the wild type enzyme has an intracellular half-life of approximately 6 h after treatment with cycloheximide
-
treatment of zebrafish embryos with 5-fluorouracil increases the enzyme stability significantly, the half life of enzyme protein is about 2.7 times longer than in untreated embryos
-