cloning of SMT gene, sequence homologies and Western Blot comparable between Leishmania donovani, Leishmania infantum and Leishmania major, serological evaluation revealed that SMT is recognized by visceral leishmaniasis (VL) patients, immunized mice shown to have lower parasite burdens in both spleens and livers compared with non-immunized mice or mice injected with adjuvant alone
cloning of SMT gene, sequence homologies and Western Blot comparable between Leishmania donovani, Leishmania infantum and Leishmania major, serological evaluation revealed that SMT is recognized by visceral leishmaniasis (VL) patients, immunized mice shown to have lower parasite burdens in both spleens and livers compared with non-immunized mice or mice injected with adjuvant alone
prediction of secondary structure, eight residues at positions 79, 81, 82, 192, 217, 218, 219 and 223 critical for catalysis, scanning mutagenesis experiments involving a leucine replacement of 52 amino acids, substitution of key residues with functionally or structurally similar amino acids, 5 new residues at positions Y192, G217, G218, T219 and Y223 can switch the course of C1-transfer activity to include plant-like C2-transfer activity, model supported in which several conserved and non-conserved amino acids located in distinct regions regulate course of the C-methylation reaction toward product differences
prediction of secondary structure, eight residues at positions 79, 81, 82, 192, 217, 218, 219 and 223 critical for catalysis, scanning mutagenesis experiments involving a leucine replacement of 52 amino acids, substitution of key residues with functionally or structurally similar amino acids, 5 new residues at positions Y192, G217, G218, T219 and Y223 can switch the course of C1-transfer activity to include plant-like C2-transfer activity, model supported in which several conserved and non-conserved amino acids located in distinct regions regulate course of the C-methylation reaction toward product differences