2.1.1.35: tRNA (uracil54-C5)-methyltransferase
This is an abbreviated version!
For detailed information about tRNA (uracil54-C5)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.35
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2.1.1.35
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histone
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h3k4me3
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trithorax
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trimethylation
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compass
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prdi-bf1
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molecular biology
- 2.1.1.35
- histone
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h3k4me3
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trithorax
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trimethylation
- compass
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prdi-bf1
- molecular biology
Reaction
Synonyms
5-methyluridine 54 tRNA methyltransferase, m5U 54 tRNA methyltransferase, m5U 54 tRNA MTase, m5U methyltransferase, m5U-methyltransferase, methyltransferase, transfer ribonucleate uracil 5-, PAB0719, PAB0719 enzyme, PabTrmU54, ribothymidyl synthase, rTase, RumA, RumA-type tRNA(uracil-54, C5)-methyltransferase, RUMT, S-adenosyl-L-methionine-dependent tRNA (uracil-54, C5)-methyltransferase, transfer RNA uracil 5-methyltransferase, transfer RNA uracil methylase, TrmA, TRMT2A, tRNA (uracil-54, C5)-methyltransferase, tRNA uracil 5-methyltransferase, tRNA(m5U54)methyltransferase, tRNA(uracil-5-)methyltransferase
ECTree
2.1.1.35 tRNA (uracil54-C5)-methyltransferaseAdvanced search results
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results (Inhibitors
Inhibitors on EC 2.1.1.35 - tRNA (uracil54-C5)-methyltransferase
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INHIBITOR 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
5-fluorouracil
exposure to 5-fluorouracil leads to extensive irreversible TRMT2A-tRNA crosslinking in vivo
DNA analog of the T-stem/loop domain
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DNA analog of the T-stem/loop domain of yeast tRNAPhe composed of d(A,U,G,C) inhibits the methylation of unfractionated methyl-deficient E. coli tRNA, significant inhibition when the concentration of tDNAPheT is twice that of the tRNA
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L-ethionine
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selective inhibitor of uracil tRNA methylase, non-competitive to S-adenosyl-L-methionine
mature tRNA
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weak, competitive inhibitor to methyl-deficient tRNA, non-competitive to S-adenosyl-L-methionine
NO2Ura-tRNA
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potent mechanism-based inhibitor, competitive with respect to tRNA, can be reversed by dilution into a large excess of tRNA substrate, forms a reversible covalent complex in which Cys-324 of RUMT is linked to the 6-position of NO2-Ura 54 in NO2-Ura-tRNA, mechanism
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5-Fluorouracil substituted tRNA
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first-order inactivation in the presence of S-adenosyl-L-methionine, potent mechanism-based irreversible inhibition, formation of a stable covalent complex containing enzyme, FUra-tRNA and the methyl group of AdoMet; mechanism of inhibition
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5-Fluorouracil substituted tRNA
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first-order inactivation in the presence of S-adenosyl-L-methionine, potent mechanism-based irreversible inhibition, formation of a stable covalent complex containing enzyme, FUra-tRNA and the methyl group of AdoMet
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5-Fluorouracil substituted tRNA
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forms inhibitory stable methylated covalent complex with RUMT
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p-chloromercuribenzoate
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at 2.5 mM, complete inhibition, completely reversed by addition of 5 mM dithiothreitol
S-adenosyl-L-homocysteine
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product inhibition, reversed by S-adenosylmethionine
S-adenosyl-L-homocysteine
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product inhibition, competitive to S-adenosyl-L-methionine
S-adenosyl-L-homocysteine
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at 0.002 mM 50% inhibition, E. coli MRE 600
S-adenosyl-L-homocysteine
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non-competitive to tRNA; product inhibition, competitive to S-adenosyl-L-methionine
S-adenosyl-L-homocysteine
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product inhibition, inhibits both methylation and H-exchange reaction
structural analogues of S-adenosyl-L-homocysteine
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inhibition competitive to S-adenosyl-L-methionine
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structural analogues of S-adenosyl-L-homocysteine
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nitrogen analogue of S-adenosyl-L-methionine AdoMDB inhibits at high concentrations methylation but increases H-exchange reaction
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additional information
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not inhibited by spermine, adenosine, L-homocysteine, snake venom phosphodiesterase
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additional information
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not inhibited by alanine, valine, ethionine sulfoxide
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