Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.1.1.34: tRNA (guanosine18-2'-O)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (guanosine18-2'-O)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.34

Reaction

S-adenosyl-L-methionine
+
guanosine18 in tRNA
=
S-adenosyl-L-homocysteine
+
2'-O-methylguanosine18 in tRNA

Synonyms

methyltransferase, transfer ribonucleate guanosine 2'-, S-adenosyl-L-methionine:tRNA (guanosine-2'-O-)-methyltransferase, SpoU, TARBP1, transfer ribonucleate guanosine 2'-methyltransferase, transfer RNA (Gm18) methyltransferase, TrmH, tRNA (Gm18) 2'-O-methyltransferase, tRNA (Gm18) methyltransferase, tRNA (guanosine-2'-O-)-methyltransferase, tRNA Gm18 methyltransferase, tRNA guanosine 2'-methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.34 tRNA (guanosine18-2'-O)-methyltransferase

Engineering

Engineering on EC 2.1.1.34 - tRNA (guanosine18-2'-O)-methyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D180A
Q9FAC4
42% of wild-type activity
D190A
Q9FAC4
5% of wild-type activity
E124A
E124D
-
small increase in Km-value
E124Q
-
no enzymic activity
E182A
Q9FAC4
29% of wild-type activity
H34A
-
no change of affinity for S-adenosyl-L-methionine and tRNA
H71A
-
has increased affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
I142A
Q9FAC4
kinetic parameters comparable to wild-type
K186A
Q9FAC4
7% of wild-type activity
K32A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
K90A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
L101A
Q9FAC4
13fold increase in Km-value
L151A
Q9FAC4
5fold increase in Km-value
L179Stop
M144A
Q9FAC4
15fold increase in Km-value
N152A
N152D
-
no enzymic activity
N152E
-
no enzymic activity
N35A
-
marked decrease in methyl transfer activity
N35D
-
marked decrease in Km-value for tRNA, low affinity for S-adenosyl-L-homocysteine
N35Q
-
dramatic increase in Km-value
P143Q
-
small increase in Km-value
Q185A
Q9FAC4
16% of wild-type activity
R109A
-
no change of affinity for S-adenosyl-L-methionine and slightly increased affinity for tRNA
R11A
-
has lost its affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R14M
-
dissociation of subunits consistent with that from the wild-type enzyme
R166A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R168A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R176A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R19A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R41A
Q9FAC4
no enzymic activity
R41K
Q9FAC4
30fold increase in Km-value
R41M
-
large increase in Km-value
R8A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
S150A
Q9FAC4
large increase in Km-value
S150C
-
Km-value similar to wild-type, decrease in Vmax
S150T
-
4fold increase in Km-value
S37G
-
5fold increase in Km-value
T99A
Q9FAC4
small increase in Km-value
V128A
Q9FAC4
small increase in Km-value
V187A
Q9FAC4
11% of wild-type activity
Y184A
Q9FAC4
10% of wild-type activity
R14M
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
dissociation of subunits consistent with that from the wild-type enzyme
-
additional information