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2.1.1.34: tRNA (guanosine18-2'-O)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (guanosine18-2'-O)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.34

Reaction

S-adenosyl-L-methionine
+
guanosine18 in tRNA
=
S-adenosyl-L-homocysteine
+
2'-O-methylguanosine18 in tRNA

Synonyms

methyltransferase, transfer ribonucleate guanosine 2'-, S-adenosyl-L-methionine:tRNA (guanosine-2'-O-)-methyltransferase, SpoU, TARBP1, transfer ribonucleate guanosine 2'-methyltransferase, transfer RNA (Gm18) methyltransferase, TrmH, tRNA (Gm18) 2'-O-methyltransferase, tRNA (Gm18) methyltransferase, tRNA (guanosine-2'-O-)-methyltransferase, tRNA Gm18 methyltransferase, tRNA guanosine 2'-methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.34 tRNA (guanosine18-2'-O)-methyltransferase

Cloned

Cloned on EC 2.1.1.34 - tRNA (guanosine18-2'-O)-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence analysis of spoU gene, encodes tRNA (Gm18) 2'-O-methyltransferase
-
expressed in Escherichia coli
-
expression in Escherichia coli with and without His-tag, amino acid sequence analysis and comparison
Q9FAC4
expression inb Escherichia coli
into vector pThioHisB and transformed into Escherichia coli strain DH5alpha, overexpressed in Escherichia coli Rosetta(DE3)/pLysS cells
vector pET30aGm expressed in Escherichia coli
-