1.9.6.1: nitrate reductase (cytochrome)
This is an abbreviated version!
For detailed information about nitrate reductase (cytochrome), go to the full flat file.
Word Map on EC 1.9.6.1
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1.9.6.1
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nitrite
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denitrification
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denitrify
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molybdenum
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paracoccus
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dissimilatory
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denitrificans
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n2o
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pantotrophus
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napf
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nitrate-reducing
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desulfuricans
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molybdoenzymes
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thiosphaera
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cyma
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fixk2
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menaquinol
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di-haem
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bismolybdopterin
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wolinella
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high-g
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narghi
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norcbqd
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nitrate-dependent
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mo-containing
- 1.9.6.1
- nitrite
-
denitrification
-
denitrify
- molybdenum
- paracoccus
-
dissimilatory
- denitrificans
- n2o
- pantotrophus
- napf
-
nitrate-reducing
- desulfuricans
-
molybdoenzymes
-
thiosphaera
-
cyma
- fixk2
- menaquinol
-
di-haem
-
bismolybdopterin
-
wolinella
-
high-g
- narghi
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norcbqd
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nitrate-dependent
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mo-containing
Reaction
2 ferrocytochrome
+
2 H+
+
Synonyms
benzyl viologen-nitrate reductase, EC 1.7.99.4, mmol_1648, More, NAP, NAP enzyme, Nap-alpha, NAP-beta, NapA, napA-beta, NapAB, NapABC, NapDAGHB, NapEDABC, nitrate reductase, periplasmic, periplasmic nitrate reductase, periplasmic nitrate reductases, reductase, nitrate (cytochrome), respiratory nitrate reductase, single subunit Nap-type periplasmic nitrate reductase
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Engineering on EC 1.9.6.1 - nitrate reductase (cytochrome)
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
C176S
S4C/S24C
site-directed mutagenesis, native, NapD results in a loss of some of the spin labels from the NapA signal peptide possibly due to the surface-exposed native cysteine residues of NapD. The NapD cysteine residues (C8 and C32) are not conserved and a cysteine-free variant of NapD complements a DELTAnapD strain for restoration of NapA activity. A NapD C8S/C32A variant remains attached to the NapA signal peptide
additional information
C176S
mutation in strictly conserved residue, significantly lower activity than wild-type
C176S
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mutation in strictly conserved residue, significantly lower activity than wild-type
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additional information
genotyping of different strains from M and G populations, overview. The only mutated gene shared between the strains from populations M and G is bll4572, this gene encodes the Bll4572 protein and is mutated in all six strains. Regulation of nos and nap genes by the NasST system in the absence or presence of nitrate in mutant strains, overview. NasS and NasT form a stable complex that is sensitive to the presence of NO3-/NO2-. Binding of NO3-/NO2- by the sensor NasS triggers the release of NasT, which binds the leader sequence upstream of the nas operon, thereby preventing hairpin formation and allowing complete transcription of the nas operon
additional information
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genotyping of different strains from M and G populations, overview. The only mutated gene shared between the strains from populations M and G is bll4572, this gene encodes the Bll4572 protein and is mutated in all six strains. Regulation of nos and nap genes by the NasST system in the absence or presence of nitrate in mutant strains, overview. NasS and NasT form a stable complex that is sensitive to the presence of NO3-/NO2-. Binding of NO3-/NO2- by the sensor NasS triggers the release of NasT, which binds the leader sequence upstream of the nas operon, thereby preventing hairpin formation and allowing complete transcription of the nas operon
additional information
Bradyrhizobium japonicum JCM 10833
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genotyping of different strains from M and G populations, overview. The only mutated gene shared between the strains from populations M and G is bll4572, this gene encodes the Bll4572 protein and is mutated in all six strains. Regulation of nos and nap genes by the NasST system in the absence or presence of nitrate in mutant strains, overview. NasS and NasT form a stable complex that is sensitive to the presence of NO3-/NO2-. Binding of NO3-/NO2- by the sensor NasS triggers the release of NasT, which binds the leader sequence upstream of the nas operon, thereby preventing hairpin formation and allowing complete transcription of the nas operon
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additional information
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the physiological role of NAP in nitrate metabolism is investigated in studies with a mutant bearing a transposon insertion in one of the structural genes
additional information
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
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the physiological role of NAP in nitrate metabolism is investigated in studies with a mutant bearing a transposon insertion in one of the structural genes
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additional information
construction of a gene nap deletion mutant, the wild-type gene is replaced by the deletion/insertion version via homologous recombination. The mutant strain can no longer grow on methanol in contrast to the wild-type
additional information
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construction of a gene nap deletion mutant, the wild-type gene is replaced by the deletion/insertion version via homologous recombination. The mutant strain can no longer grow on methanol in contrast to the wild-type
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additional information
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constructin of a napA mutant strain CAL277, and of several other nap gene mutants, overview
additional information
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constructin of a napA mutant strain CAL277, and of several other nap gene mutants, overview
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