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1.8.5.4: bacterial sulfide:quinone reductase

This is an abbreviated version!
For detailed information about bacterial sulfide:quinone reductase, go to the full flat file.

Word Map on EC 1.8.5.4

Reaction

n HS- + n quinone =

Polysulfide
+ n quinol

Synonyms

CmSQR, CpSQR, CT1087, HMT2, III SQR, membrane-bound sulfide:quinone oxidoreductases, SQOR, SQR, Sqrdl, sqrF, Suden_1879, Suden_2082, Suden_619, sulfide quinone oxidoreductase, sulfide quinone reductase, sulfide-quinone oxidoreductase, sulfide-quinone reductase, sulfide: quinone oxidoreductase, sulfide:decylubiquinone oxidoreductase, sulfide:quinone oxidoreductase, sulfidequinone reductase-like protein, TrSqrF, type I SQR, type III sulfide:quinone oxidoreductase, type VI sulfide:quinone oxidoreductase

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.5 With a quinone or similar compound as acceptor
                1.8.5.4 bacterial sulfide:quinone reductase

Crystallization

Crystallization on EC 1.8.5.4 - bacterial sulfide:quinone reductase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 2.2 M NH4H2PO4 and 100 mM Tris-HCl, pH 8.5 (final pH of the solution is 4.5), or 2.2 M NH4H2PO4/K2HPO4 at pH 4.5
-
crystal structures of the wild-type enzyme in complex with sodium selenide and gold(I) cyanide. Mechanism for the reduction of sulfides to elemental sulfur may involve nucleophilic attack of Cys356 on C4A atom of FAD or alternatively, an alternative anionic radical mechanism by direct electron transfer from Cys356 to the isoalloxazine ring of FAD
hanging drop vapor diffusion method, using 30% (w/v) PEG 600, 0.1 M bis-Tris pH 5.5, 0.1 M ammonium sulfate
-
native or in complex with decylubiquinone, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol 600, 0.1 M 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol (pH 5.5 or pH 6.5), 0.1 M magnesium sulfate, and 0.05% (w/v) n-dodecyl beta-D-maltoside
crystallization in two crystal forms of hexagonal, prism shape and thin, elongated shape
hanging drop vapor diffusion method, using 2.0 M ammonium sulfate and 4% (v/v) PEG 400