1.8.4.11: peptide-methionine (S)-S-oxide reductase
This is an abbreviated version!
For detailed information about peptide-methionine (S)-S-oxide reductase, go to the full flat file.
Reaction
Synonyms
EC 1.8.4.6, ecdysone-induced protein 28/29 kDa, FMsr, LIC_10545, LIC_12978, linmsra1, linmsra2, LMJF_07_1140, methionine S-oxide reductase (S-form oxidizing), methionine sulfoxide reductase, methionine sulfoxide reductase A, methionine sulfoxide reductases A, methionine sulfoxide-S-reductase, methionine sulphoxide reductase, methionine sulphoxide reductase A, methionine-S-sulfoxide reductase, MetSO-L12 reductase, More, mrsA, MSR, MSR10, MSR180, MsrA, MSRA-1, MsrA/B, MsrA/MsrB, MsrA1, MSRA2, MSRA4, msrAB, MsrABTk, MsrBA, Peptide Met(O) reductase, peptide methionine S-sulfoxide reductase, peptide methionine sulfoxide reductase, peptide methionine sulfoxide reductase A, peptide methionine sulfoxide reductase type A, peptide methionine sulphoxide reductase, peptide-methionine (S)-S-oxide reductase, peptide-methionine sulfoxide reductase, PilA, PilB, PilB protein, PMSR, PMSRA, protein-methionine-S-oxide-reductase, sulindac reductase, TbmsrA, TCDM_14270
ECTree
1.8.4.11 peptide-methionine (S)-S-oxide reductaseAdvanced search results
results (
results (KM Value
KM Value on EC 1.8.4.11 - peptide-methionine (S)-S-oxide reductase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.197
Calmodulin
-
myristoylated enzyme, in 20 mM Tris-HCl, pH 7.5, 1 mM CaCl2, at 37°C
1.18
dabsyl L-methionine-(S)-sulfoxide
pH 7.4, 37°C, recombinant enzyme
1.1
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide
recombinant isozyme PMSRA4, with DTT, pH 8.0, 22°C
2.12
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide
recombinant isozyme PMSRA3, with DTT, pH 7.5, 22°C
0.3
dabsyl-L-methionine (S)-S-oxide
isolated MrsA domain, pH 7.5, 37°C
1.03
dabsyl-L-methionine (S)-S-oxide
mutant Y163A//E165A, pH 7.5, 37°C
0.04
dabsyl-L-methionine (S)-sulfoxide
pH 7.5, 37°C, mutant enzyme E339A
0.12
dabsyl-L-methionine (S)-sulfoxide
pH 7.5, 37°C, mutant enzyme E193A/D197A
0.12
dabsyl-L-methionine (S)-sulfoxide
pH 7.5, 37°C, mutant enzyme Y343F
0.14
dabsyl-L-methionine (S)-sulfoxide
pH 7.5, 37°C, mutant enzyme E193A
0.15
dabsyl-L-methionine (S)-sulfoxide
pH 7.5, 37°C, mutant enzyme D197A
0.17
dabsyl-L-methionine (S)-sulfoxide
pH 7.5, 37°C, wild-type enzyme
0.34
dabsyl-L-methionine-(S)-S-oxide
-
recombinant wild-type MsrA, with DTT, pH 7.5, 37°C
4.3
dabsyl-L-methionine-(S)-S-oxide
-
recombinant truncated MsrA DELTA(1-46), with DTT, pH 7.5, 37°C
4
dabsylated L-methionine (S)-sulfoxide
-
purified cysteine mutant form U16C, using dithiothreitol as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C
10.2
dabsylated L-methionine (S)-sulfoxide
-
wild type enzyme, using dithiothreitol as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C
15
dithiothreitol
-
MsrA domain of MsrABTk, in the presence of 80 mM L-methionine (S)-sulfoxide
0.044
L-methionine (S)-sulfoxide
-
recombinant wild-type enzyme, pH 7.5, 37°C
0.069
L-methionine (S)-sulfoxide
-
recombinant enzyme. isoform MSRA, at 30°C and pH 7.5
0.12
L-methionine (S)-sulfoxide
-
recombinant wild-type enzyme, pH 7.5, 37°C
0.723
L-methionine (S)-sulfoxide
-
recombinant enzyme. isoform MSR10, at 30°C and pH 7.5
19
L-methionine (S)-sulfoxide
-
MsrA domain of MsrABTk, in the presence of 100 mM dithiothreiol
0.135
L-methionine-(S)-S-oxide
30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S
0.39
L-methionine-(S)-S-oxide
30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme
0.42
L-methionine-(S)-S-oxide
pH 7.5, 30°C, cosubstrate: tryparedoxin I
0.55
L-methionine-(S)-S-oxide
30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme
0.0249
N-acetyl-L-methionine-(S)-S-oxide
30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme
0.28
N-acetyl-L-methionine-(S)-S-oxide
30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S
0.86
N-acetyl-L-methionine-(S)-S-oxide
pH 7.5, 30°C, cosubstrate: tryparedoxin I
1.4
N-acetyl-L-methionine-(S)-S-oxide
30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme
0.0018
thioredoxin
30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme
0.002
thioredoxin
30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme
0.00322
thioredoxin
wild type isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C
0.00343
thioredoxin
mutant C180S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C
0.006
thioredoxin
pH 7.5, 30°C, cosubstrate: L-methionine-(S)-S-oxide
0.0189
thioredoxin
mutant C188S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C
0.019
thioredoxin
30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme
0.0218
thioredoxin
wild type isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C
0.02265
thioredoxin
mutant C180S of isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C
0.0233
thioredoxin
mutant C194S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C
0.0305
thioredoxin
30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme
0.0404
thioredoxin
-
in 20 mM Tris-HCl, pH 8.0, 30 mM KCl, 10 mM MgCl2, at 37°C
0.013
tryparedoxin I
-
recombinant enzyme. isoform MSRA, at 30°C and pH 7.5
-
0.017
tryparedoxin I
pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide
-
0.033
tryparedoxin I
-
recombinant enzyme. isoform MSR10, at 30°C and pH 7.5
-
additional information
additional information
-
kinetics of disulfide formation in MsrA at Cys52/Cys198 at pH 5.5 and pH 8.0, 25°C, single turnover experiments, steady-state kinetics
-
additional information
additional information
-
detailed kinetics, wild-type and mutants MsrAs
-
additional information
additional information
-
steady-state and reductase step kinetic parameters of wild-type and mutated MsrAs
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