1.8.4.10: adenylyl-sulfate reductase (thioredoxin)

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For detailed information about adenylyl-sulfate reductase (thioredoxin), go to the full flat file.

Reaction

Synonyms

5'-adenylylsulfate reductase, adenosine-5'-phosphosulfate reductase, adenylylsulfate reductase, APR, APR-B, APS reductase, PaAPR, PpAPR-B, thioredoxin dependent 5'-adenylylsulfate reductase, thioredoxin-dependent APS reductase

ECTree

     1 Oxidoreductases

         1.8 Acting on a sulfur group of donors

             1.8.4 With a disulfide as acceptor

                1.8.4.10 adenylyl-sulfate reductase (thioredoxin)

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Results	in table
1	Activating Compound
17096	AA Sequence
1	Application
1	CAS Registry Number
7	Cloned(Commentary)
22	Cofactor
2	Crystallization (Commentary)
1	General Stability
7	Inhibitors
7	kcat/KM [mM/s]
1	Ki Value [mM]
17	KM Value [mM]
9	Metals/Ions
6	Molecular Weight [Da]
25	Natural Substrates/ Products (Substrates)
32	Organism
1	Pathway
10	PDB ID
4	pH Optimum
1	pH Stability
8	Protein Variants
4	Purification (Commentary)
2	Reaction
3	Reaction Type
31	Reference
1	Source Tissue
2	Storage Stability
52	Substrates and Products (Substrate)
4	Subunits
17	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
9	Turnover Number [1/s]

Engineering

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Engineering on EC 1.8.4.10 - adenylyl-sulfate reductase (thioredoxin)

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

D122G

Physcomitrium patens

Q8L5D0

mutant does not display improved activity with 3'-phosphoadenosine-5'-phosphosulfate and shows significantly reduced activity with 5'-adenylylsulfate as the substrate

742508

C139S

Pseudomonas aeruginosa

-

no activity

667787

C140S

Pseudomonas aeruginosa

-

mutant enzyme shows 3% of wild-type activity

667787

C228S

Pseudomonas aeruginosa

-

no activity

667787

C231S

Pseudomonas aeruginosa

-

no activity

667787

C256S

Pseudomonas aeruginosa

-

no activity

667787

additional information

2 entries

additional information

Physcomitrium patens

-

construction of APR-B knockout plants, the mutant plants are able to grow on sulfate as a sole sulfur source, and the content of low molecular weight thiols is not different from wild-type plants. However, when treated with low concentrations of cadmium, the APR-B knockout plants are more sensitive than wild-type plants, phenotype, overview

694695

additional information

Pseudomonas aeruginosa

-

a heterologous system is constructed in which the C domain of EiAPR (EC 1.8.4.9) is fused to the carboxyl terminus of the APS reductase from Pseudomonas aeruginosa (EC 1.8.4.10), an enzyme that normally uses thioredoxin as an electron donor and is incapable of using glutathione for this function. The hybrid enzyme, which retains the [4Fe-4S] cluster from PaAPR, can use both thioredoxin and glutathione as an electron donor for APS reduction. The ability to use glutathione is enhanced by the addition of Na2SO4 to the reaction buffer, a property that the hybrid enzyme shares with EiAPR

667746