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1.8.1.7: glutathione-disulfide reductase

This is an abbreviated version!
For detailed information about glutathione-disulfide reductase, go to the full flat file.

Word Map on EC 1.8.1.7

Reaction

2 glutathione +

NADP+
=
glutathione disulfide
 +
NADPH
 +
H+

Synonyms

At3g54660, EC 1.6.4.2, GLR, glutahione reductase, glutathione disulfide reductase, glutathione reductase, glutathione reductase (NADPH), glutathione reductase 3, glutathione reductase Glr1, glutathione S-reductase, glutathione: NADP(+) oxidoreductase, glutathione: NADP+ oxidoreductase, glutathione:NADP+ oxidoreductase, Gor, GOR1, GOR2, GR, GR1, GR2, Gr3, GRase, GRase-1, GRd, GSH reductase, GSHR, Gsr, GSR-1, GSSG reductase, HCOI_01258400, hGR, HvGR1, HvGR2, multifunctional thioredoxin-glutathione reductase, NADPH-glutathione reductase, NADPH-GSSG reductase, NADPH-reduced GR, NADPH: oxidized glutathione oxidoreductase, NADPH:oxidized glutathione oxidoreductase, NADPH:oxidized-glutathione oxidoreductase, OBP29, PfGR, psgr, PtGR1.1, PtGR1.2, PtGR2, reductase, glutathione, SpGR, TaGR1, TaGR2, TGR, thioredoxin glutathione reductase, thioredoxin/glutathione reductase, TrxR3

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.1 With NAD+ or NADP+ as acceptor
                1.8.1.7 glutathione-disulfide reductase

Purification

Purification on EC 1.8.1.7 - glutathione-disulfide reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1714fold from liver homogenate, by ammonium sulfate fractionation, affinity chromatography, and gel filtration
-
2',5'-ADP agarose affinity chromatography and DEAE Sephadex gel filtration
-
2',5'-ADP Sepharose 4B affinity chromatography and DEAE Sepharose column chromatography
-
31250fold from erythrocyte hemolysate, by ammonium sulfate fractionation, affinity chromatography, and gel filtration
-
5000fold from brain to homogeneity in several steps, overview
-
5342fold to homogeneity from erythrocytes by hemolysis, ammonium sulfate fractionation, affinity chromatography, and gel filtration
-
647fold to homogeneity from metacystodes by ammonium sulfate fractionation, ion exchange chromatography, affinity chromatography, and gel filtration
-
amino acid sequence analysis
-
ammonium sulfate fractionation, 2',5'-ADP Sepharose 4B affinity chromatography and Sephadex G-200 gel filtration
ammonium sulfate precipitation, 2 ',5'-ADP Sepharose-4B affinity column chromatography and Sephadex G-200 gel filtration
-
ammonium sulfate precipitation, 2',5'-ADP Sepharose 4B column chromatography and Sephadex G-200 gel filtration
-
ammonium sulfate precipitation, 2‘‘,5‘-ADP Sepharose 4B affinity column chromatography, and Sephadex G-200 gel filtration
-
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, Blue-A Sepharose column chromatography, and 2',5'-ADP-Sepharose column chromatography
-
ammonium sulfate precipitation, ultrafiltration, CM-Sepharose column chromatography, DEAE-Sepharose column chromatography, GSH–Sepharose affinity column chromatography, and ADP-Sepharose affinity chromatography
-
chloroplast enzyme
-
color of the purified enzyme is yellow, and it has an absorption spectrum characteristic of flavoproteins
-
enzyme expressed from plasmid, small and large scale
-
from erythrocyte
-
from lens and erythrocytes
-
from temperature hardened and nonhardened strains
-
heat-denaturation and Sephadex G25 gel filtration, 2',5'-ADP agarose 4B column chromatography, and PBE94 column chromatography
-
ion exchange chromatography on hydroxylapatite, LKB 8101 isoelectrofocusing column chromatography, Sephacryl S-300 column gel filtration and Matrix Red dye ligand chromatography
-
ion exchange column chromatography and gel filtration
-
large scale
-
Mono Q HR 5/5 column chromatography and 2'5'-ADP Sepharose 4B affinity chromatography
-
native enzyme from microplasmodia, 968fold in a multistep procedure
Ni-NTA affinity column chromatography
-
Ni-NTA agarose resin column chromatography
Ni2+-NTA column chromatography
-
nickel affinity column chromatography
partially
recombinant His-tagged enzyme from in-vitro expression by nickel chelate affinity chromatography
-
recombinant mutant from Escherichia coli
to homogeneity from brain
-
via high-level expression of recombinant enzyme in Escherichia coli
-
wild-type and recombinant from Escherichia coli
-