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1.8.1.7: glutathione-disulfide reductase

This is an abbreviated version!
For detailed information about glutathione-disulfide reductase, go to the full flat file.

Word Map on EC 1.8.1.7

Reaction

2 glutathione +

NADP+
=
glutathione disulfide
 +
NADPH
 +
H+

Synonyms

At3g54660, EC 1.6.4.2, GLR, glutahione reductase, glutathione disulfide reductase, glutathione reductase, glutathione reductase (NADPH), glutathione reductase 3, glutathione reductase Glr1, glutathione S-reductase, glutathione: NADP(+) oxidoreductase, glutathione: NADP+ oxidoreductase, glutathione:NADP+ oxidoreductase, Gor, GOR1, GOR2, GR, GR1, GR2, Gr3, GRase, GRase-1, GRd, GSH reductase, GSHR, Gsr, GSR-1, GSSG reductase, HCOI_01258400, hGR, HvGR1, HvGR2, multifunctional thioredoxin-glutathione reductase, NADPH-glutathione reductase, NADPH-GSSG reductase, NADPH-reduced GR, NADPH: oxidized glutathione oxidoreductase, NADPH:oxidized glutathione oxidoreductase, NADPH:oxidized-glutathione oxidoreductase, OBP29, PfGR, psgr, PtGR1.1, PtGR1.2, PtGR2, reductase, glutathione, SpGR, TaGR1, TaGR2, TGR, thioredoxin glutathione reductase, thioredoxin/glutathione reductase, TrxR3

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.1 With NAD+ or NADP+ as acceptor
                1.8.1.7 glutathione-disulfide reductase

General Stability

General Stability on EC 1.8.1.7 - glutathione-disulfide reductase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
1 mM EDTA is required
-
active in the presence of imidazole up to 0.8 M, the native enzyme is resistant to digestion by trypsin or chymotrypsin
-
complete inactivation after incubation with NADH, reversible by addition of GSSG
-
complete inactivation after incubation with NADPH, reversible by addition of GSSG
concentrated solution, stable for years
crystals and amorphous protein obtained by precipitation with ammonium sulfate are stable
dichloroindophenol protects against thermal inactivation at 80°C
-
dithiothreitol stabilizes
-
FAD, no stabilization against thermal inactivation at 80°C
-
FAD, stabilizes
-
freezing denaturates
glutathione protects against thermal inactivation at 80°C
-
glutathione stabilizes chloroplast enzymes
-
glycerol stabilizes
-
GSSG protects against thermal inactivation at 80°C
-
NADP+ stabilizes
NADP+, stabilizes against thermal inactivation at 80°C
-
NADPH is not protective against induced inactivation by peroxynitrite
-
partial inactivation after incubation with ATP
-
pure enzyme heated in presence of bovine serum albumin and 0.020 mM NADPH: loss of activity beyond 30°C, significant reduction even at 0°C
-
repeated freezing and thawing, inactivation
repeated freezing and thawing, no effect
-
repeated freezing and thawing, stable
urea, activation from 0.4-0.6 M, inactivation at higher concentration
-
urea, quite stable to