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1.8.1.4: dihydrolipoyl dehydrogenase

This is an abbreviated version!
For detailed information about dihydrolipoyl dehydrogenase, go to the full flat file.

Word Map on EC 1.8.1.4

Reaction

protein N6-(dihydrolipoyl)lysine
+
NAD+
=
protein N6-(lipoyl)lysine
+
NADH
+
H+

Synonyms

apicoplast E3, CDS, CIP50, coronin-interacting protein, dehydrogenase, lipoamide, dehydrolipoate dehydrogenase, DHLDH, DHLipDH, diaphorase, dihydrolipoamide dehydrogenase, dihydrolipoamide dehydrogenase E3, dihydrolipoamide:NAD+ oxidoreductase, dihydrolipoic dehydrogenase, dihydrolipomide dehydrogenase, dihydrolipoyl dehydrogenase, DLD, DLD1, Dld2, DLDH, DLDH dehydrogenase, DLDH diaphorase, DLDH2, DT-diaphorase, E3, E3 component, E3 component of 2-oxoglutarate dehydrogenase complex, E3 component of acetoin cleaving system, E3 component of alpha keto acid dehydrogenase complexes, E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes, E3 component of pyruvate complex, E3 lipoamide dehydrogenase, E3 protein component of 2-oxoacid dehydrogenase multienzyme complexes, E3 subunit of the alpha-ketoglutarate dehydrogenase complex, EC 1.6.4.3, Glycine cleavage system L protein, Glycine oxidation system L-factor, hDLDH, hE3, hLADH, L-protein, LAD, LADH, LDH, LDP-Glc, LDP-Val, LipDH, lipoamide dehydrogenase, lipoamide dehydrogenase (NADH), lipoamide dehydrogenase C, lipoamide dehydrogenase2, lipoamide oxidoreductase (NADH), lipoamide reductase, lipoamide-dehydrogenase-valine, lipoate dehydrogenase, lipoic acid dehydrogenase, lipoyl dehydrogenase, LPD, LPD-GLC, LPD-VAL, LPD1, LPD2, Lpd3, LpdA, LpdC, LpdG, LpdV, More, mtLPD2, NAD(P)H:lipoamide oxidoreductase, NADH dehydrogenase, NADH diaphorase, NADH:lipoamide oxidoreductase, nicotinamide adenine dinucleotide diaphorase, ORF-E3, pdhD, pdhL, PfaE3, rhDLDH, TAase, ubiquinone reductase

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.1 With NAD+ or NADP+ as acceptor
                1.8.1.4 dihydrolipoyl dehydrogenase

Purification

Purification on EC 1.8.1.4 - dihydrolipoyl dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography on propyllipoamide-glass columns
ammonium sulfate precipitation
-
ammonium sulfate precipitation and Ni-NTA agarose column chromatography
Starkeyomyces koorchalomoides
-
by gel filtration
DEAE-Toyopearl 650 M column chromatography, butyl-Toyopearl 650 M column chromatography, and TSK-gel G3000SW gel filtration
dissociation of the lipoamide dehydrogenase component from the branched-chain alpha-keto acid dehydrogenase complex during purification
-
enzmye from culture supernatant of Clostridium kluyveri is purified using a DEAE-toyopearl column and a TSK-GEL G-3000 column. Recombinant protein is purified using a HiTrap chelating HP column
-
from pyruvate dehydrogenase complex
further purification of the commercial preparation
-
further purification of the commercial preparation by gel filtration
-
gel filtration
gel filtration, at least 90% pure
-
HiTrap Q column chromatography
-
metal-affinity chromatography and Superdex 200 gel filtration chromatography
Ni-NTA agarose column chromatography, gel filtration
-
Ni-NTA column chromatography
Ni-NTA His-Bind resin column chromatography
Ni-NTA resin column chromatography and gel filtration
-
Ni-NTA Sepharose column chromatography
-
Ni-NTA-agarose resin column chromatography and Superdex 200 gel filtration
nickel affinity column chromatography
nickel iminodiacetic acid Sepharose 6B column chromatography
-
partially
-
presence of dithiothreitol during purification preserves enzymatic activity
-
purified from strain ASKA clone (-) JW0112
-
purified using copper-based metal ion affinity chromatography in the presence of 2 M KCl
recombinant D473L mutant from Escherichia coli to homogeneity
-
recombinant enzyme
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation and hydrophobic interaction chromatography to over 98% purity
-
recombinant His-tagged E3 wild-type and mutant proteins
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain NovaBlue (DE3) by nickel affinity chromatography and gel filtration
-
recombinant His-tagged truncated isozymes from Escherichia coli by nickel affinity chromatography to 98% homogeneity
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration. Purification of native pyruvate and2-oxoglutarate dehydrogenase complexes from strain PA14 by hydroxyapatite chromatography and gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain DH5alpha by nickel affinity chromatography and dialysis
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain XL-1 Blue by nickel affinity chromatography and dialysis
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatography and gel filtration
recombinant mutant T44V from Escherichia coli strain XL1-Blue
-
recombinant N-terminally GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
Sephacryl S-100 column gel filtration
-
Sephadex G-25 column gel filtration
Strep-Tactin Sepharose column chromatography
Superdex HR-200 gel filtration
-
wild-type and mutant enzymes K37E, H452Q and E457Q
-
wild-type and mutant enzymes K54E, S53K54-K53S54 and E192Q
-
wild-type and mutant proteins, highly purified
wild-type DLD and mutants purified by nickel affinity chromatography. C-term DLD purified to near homogeneity by a five-step procedure
-