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1.8.1.12: trypanothione-disulfide reductase

This is an abbreviated version!
For detailed information about trypanothione-disulfide reductase, go to the full flat file.

Word Map on EC 1.8.1.12

Reaction

trypanothione
+
NADP+
=
trypanothione disulfide
 +
NADPH
 +
H+

Synonyms

EC 1.6.4.8, LbTryR, LdTryR, Li-TryR, LiTR, N(1),N(8)-bis(glutathionyl)spermidine reductase, NADPH2:trypanothione oxidoreductase, TbTR, TCDM_11669, TcTR, TPR, TR, trypanothione disulfide reductase, trypanothione reductase, trypanothione-disulfide reductase, TryR

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.1 With NAD+ or NADP+ as acceptor
                1.8.1.12 trypanothione-disulfide reductase

Purification

Purification on EC 1.8.1.12 - trypanothione-disulfide reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with inhibitor 4-((1-(4-ethylphenyl)-2-methyl-5-(4-(methylthio)phenyl)-1H-pyrrol-3-yl)methyl)thiomorpholine
Ni-affinity column chromatography
-
Ni-NTA column chromatography
partial, cell fractionation
-
recombinant enzyme is purified by ammonium sulfate precipitation, 2'5'-ADP Sepharose affinity column chromatography, and anion exchange chromatography
recombinant from Escherichia coli
-
recombinant mutant from Escherichia coli
-
recombinant overexpression from plasmid in Leishmania donovani and in Trypanosoma cruzi
-
recombinant overexpression of the enzyme from plasmid
-
recombinant protein by Ni2+-charged chelating sepharose fast flow
recombinant soluble glutathione-S-transferase fusion protein from Escherichia coli strain BL21(DE3) to homogeneity, 20fold
recombinant wild-type and mutants C53S, C53A, C58S from Escherichia coli
-
wild-type and mutants recombinant from Escherichia coli
-